Wild seed selecting and seed breeding method for anoectochilus roxburghii

A clematis, field technology, applied in the biological field, can solve the problems of high cultivation cost, easy rotten root system, easy yellowing of clematis leaves, etc., and achieve the effect of improving the germination rate

Active Publication Date: 2014-05-14
FUJIAN LANSHUI LOTUS BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Traditional clematis artificial cultivation uses soil or sand as the cultivation substrate for cultivation and cultivation, and the fresh clematis received per square meter is 0.36kg. The traditional cultivation method of clematis not only has high cultivation costs, but also often produces clematis leaves that are easy to Yellow, root system is easy to rot, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1) Selection of explants

[0021] In September every year, when clematis blooms, we select wild blooming and robust clematis with excellent traits. After artificial pollination, fruit clips are formed; 50 days after pollination, plump and unbroken fruit clips are picked. as explants;

[0022] 2) Aseptic manipulation of explants

[0023] After cleaning the picked fruit clips, carry out aseptic operation, disinfect the outside of the fruit clips in the aseptic operating table, put the fruit clips into a bleach solution with a mass fraction of 12% and shake them for 3 minutes, then let them stand for 5 minutes. Minutes later, shake for another 2 minutes, and the whole process takes 10 minutes to complete the sterilization of the outer fruit clip; open the fruit clip, and inoculate the seeds inside on the clematis culture medium;

[0024] 3) Cultivation steps

[0025] Place the inoculated explants at a constant temperature of 24°C and culture them in the dark for 10 days...

Embodiment 2

[0028] 1) Selection of explants

[0029] Between September and November every year, when clematis blooms, we select wild clematis that have blossomed and have excellent traits, and after artificial pollination, they form fruit clips; picking and picking 45 days after pollination are full , unruptured fruit clips as explants;

[0030] 2) Aseptic manipulation of explants

[0031] After cleaning the picked fruit clips, carry out aseptic operation, disinfect the outside of the fruit clips in the aseptic operating table, put the fruit clips into a bleach solution with a mass fraction of 15% and shake them for 5 minutes, and then let them stand for 7 minutes. Minutes later, shake for another 3 minutes, and the whole process takes 15 minutes to complete the sterilization of the outer fruit clip; open the fruit clip, and inoculate the seeds inside on the clematis culture medium;

[0032] 3) Cultivation steps

[0033] Place the inoculated explants at a constant temperature of 26°C a...

Embodiment 3

[0036] 1) Selection of explants

[0037] Between September and November every year, when clematis blooms, we select wild clematis that have bloomed and have excellent characteristics, and after artificial pollination, they form fruit clips; picking and picking the full-bodied clematis 55 days after pollination , unruptured fruit clips as explants;

[0038] 2) Aseptic manipulation of explants

[0039] After cleaning the picked fruit clips, carry out aseptic operation, disinfect the outside of the fruit clips in the aseptic operating table, put the fruit clips into a bleach solution with a mass fraction of 14% and shake them for 4 minutes, then let them stand for 6 minutes. Minutes later, shake for another 2 minutes, and the whole process takes 12 minutes to complete the sterilization of the outer fruit clip; open the fruit clip, and inoculate the seeds inside on the clematis culture medium;

[0040] 3) Cultivation steps

[0041] Place the inoculated explants at a constant te...

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PUM

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Abstract

The invention relates to a wild seed selecting and seed breeding method for anoectochilus roxburghii and belongs to the technical field of biology. The wild seed selecting and seed breeding method comprises the following steps: selecting wild anoectochilus roxburghii which has good properties and is blossomed and strong; carrying out artificial pollination to form fruit pods; picking the fruit pods which are full and are not cracked as explants; inoculating seeds in the fruit pods into a culture medium to be cultured under sterile operation; finally, entering an industrialized propagation phase. The survival rate of the anoectochilus roxburghii which is subjected to seed selection and seed breeding can be up to more than 99%.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for field selection and breeding of clematis. Background technique [0002] Clematis ( Anoectochilus roburghii ) is a perennial rare Chinese herbal medicine of the Orchidaceae lipped plant Mosaicus genus, also known as golden thread orchid, golden thread grass, golden grass, bird ginseng, golden thread, black ginseng, golden thread tiger's head plantain, money grass, golden thread Lycopodium, etc., Distributed in Fujian, Guangdong, Guangxi, Hainan, Sichuan, Guizhou, and Yunnan, where there are evergreen broad-leaved trees, stone walls, and moist areas with loose soil. Clematis clematis is widely used among the people, and it is known as "King of Medicine", "Golden Grass", "God Grass", "Bird Ginseng" and other laudatory titles. Aurantia clematis is a perennial rare Chinese herbal medicine. The whole herb is used as medicine, clearing heat and cooling blood, deh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 黄艳丽王胜专
Owner FUJIAN LANSHUI LOTUS BIOLOGICAL TECH
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