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Human skin module and application thereof

A skin model and skin technology, applied in the fields of tissue cytology and tissue engineering, can solve problems such as regenerating skin, and achieve the effect of promoting wound healing and treating skin aging.

Active Publication Date: 2015-02-25
JINAN PANSHENG BIOTECH
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0005] In conclusion, at present, there is no one in the world that can use in vitro cultured and expanded cells to regenerate fully functional human skin with mature hair

Method used

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  • Human skin module and application thereof
  • Human skin module and application thereof

Examples

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example

[0024] Example: Regeneration of functional human skin and hair follicles in nude mice using in vitro culture-expanded cells

[0025] Materials and methods:

[0026]Animals: Immunodeficient nude mice (or homozygous nu / nu mutant mice) or non-diabetic obese mice / severe combined immunodeficiency mice are albino mice, both of which are immune-deficient to reduce the rejection of foreign tissues, etc. Therefore, it is suitable for various xenograft tissue transplantation and tumor transplantation.

[0027] Cells and Cell Culture Media: Dissociated epidermal cells were obtained from human neonatal foreskins. Dermal cells come from the scalp of a 15-week-old fetus. After the cells were isolated from the tissue, they were cultured in dermal cell medium DMEM / F12 (1:1) (Gibco, Cat. #11039). Epidermal cells were cultured in CNT07 medium containing protein kinase (ROCK) inhibitor Y27632. The dermal cells and epidermal cells need to be cultured to the third cycle and the third generati...

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Abstract

The invention discloses a method for regenerating human intact skin tissue by use of in vitro cultured cells. The method comprises separately culturing epidermal cells and dermal cells derived from fetus scalp, newborn prepuce or adult scalp, and subculturing, wherein the used culture medium and culture method can maintain multiple functions of skin cells; separately digesting the cultured and proliferated multifunctional epidermal cells and dermal cells into single cells, and mixing to obtain suspension; transferring onto a semipermeable polymer or silica gel membrane substrate for incubation; transplanting cells on adhesive film to a receptor. The method can regenerate human intact functional skin and hair, the regenerated skin contains an epidermal layer, a dermal layer and a subcutaneous tissue as well as skin appendant organs. The skin and hair model regenerated by the method disclosed by the invention can be used for study of human skin and hair diseases, screening of skin drugs, and development of cell products for treating wound, alopecia and other skin diseases.

Description

technical field [0001] The invention relates to a skin model and the application of the model in the research of human skin diseases, the screening of skin medicines and the development of clinical products for treating wounds. Belongs to the field of histocytology and tissue engineering. Background technique [0002] Dermatologists and skin surgeons have long struggled to regenerate fully functioning skin clinically for the healing of skin trauma and the removal of skin scars. There are many reasons for skin damage: such as genetic diseases, burns, chronic trauma such as trauma caused by diabetes. Many skin damages cannot be recovered by themselves, such as deep large-scale burns, and large-area damage to the skin can easily lead to infection and death. At present, the most common method of treatment is to transplant the uninjured skin of other parts of oneself to the burn and scald with surgery. However, this method has great limitations. On the one hand, if a large are...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/60A61K49/00
Inventor 吴训伟布莱恩·马歇尔
Owner JINAN PANSHENG BIOTECH
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