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Preparation method of anti-osteoporosis composition

A technology for osteoporosis and composition, which is applied in the field of preparation of pharmaceutical composition, can solve the problems of bone tissue becoming brittle and high price, and achieve the effects of less side effects, safe use, and simple extraction method

Inactive Publication Date: 2014-05-28
邢秋苓
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Osteogenesis and bone resorption maintain a good balance when young, but as we age, the balance tends to bone resorption due to various reasons, and if this state continues for a long time, the bone tissue becomes brittle, leading to osteoporosis disease, fractures and other bone diseases
[0003] At present, a variety of drugs and methods for the prevention and treatment of osteoporosis have been developed, but due to the high price and various side effects caused by long-term use, there are certain limitations in use.

Method used

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  • Preparation method of anti-osteoporosis composition
  • Preparation method of anti-osteoporosis composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation method of anti-osteoporosis composition, formula is:

[0025] Safflower 8g, Astragalus 5g, Pueraria 7g, Rehmannia glutinosa 4g, Eucommia 5g.

[0026] Concrete preparation method comprises the following steps:

[0027] ① Weigh safflower, astragalus, kudzu root, rehmannia glutinosa and Eucommia ulmoides according to the above formula, wash, crush into small pieces of 1-10mm, and set aside;

[0028] ② Mix the crushed safflower, astragalus and kudzu root, extract twice with 75% ethanol under reflux, 1.5 hours each time, combine the extracts, refrigerate overnight, filter, and recover ethanol from the filtrate under reduced pressure to obtain ethanol extract;

[0029] ③ Add 5 times the amount of distilled water to the ethanol extract to dissolve it, then extract it with ethyl acetate, recover the solvent under reduced pressure, and obtain the extract I;

[0030] ④ Dissolve the extract I with 5 times the amount of ethanol, refrigerate overnight, filter, and ...

Embodiment 2

[0036] Using the composition obtained in Example 1, its effect on osteoclast formation was determined.

[0037] Osteoclast formation system: Multinucleated osteoclasts can be formed by culturing RAW264.7 cells in α-MEM medium for 3-5 days in the presence of RANKL. The formed osteoclasts were observed with tartrate-resistant acid phosphatase (TRAP) staining method to observe TRAP-positive multinucleated cells, and the number of TRAP-positive multinucleated cells with more than 3 nuclei under the microscope was used as an indicator of osteoclast formation.

[0038] The RAW264.7 is a mouse mononuclear / macrophage cell line, which can form into osteoclasts with bone resorption capacity in the presence of osteoclast formation factor (RANKL).

[0039] The anti-osteoporosis composition extract was dissolved in α-MEM culture medium, prepared to a certain concentration, and then added to the osteoclast formation system of RAW264.7 cells, in a 5% carbon dioxide, 37°C cell culture box Af...

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Abstract

The invention provides a preparation method of an anti-osteoporosis composition. The composition comprises the following ingredients in parts by weight: 2-10 parts of safflower, 3-10 parts of radix astragali, 1-10 parts of radix puerariae, 3-8 parts of prepared rhizome of rehmannia, and 4-7 parts of the bark of eucommia. The preparation method comprises the following steps: utilizing a multi-organic solvent extraction method and a separation method to process ingredients with the effect of inhibiting osteoclast formation from the raw materials, and combining the processed ingredients to obtain the anti-osteoporosis composition. The composition has good effect of inhibiting osteoclast formation, is derived from natural herbs, has fewer side effects, and is safe in use. In addition, the composition is scientific and reasonable in formula; the ingredients are rich in the resource, and the extraction method is simple; the industrial production is facilitated.

Description

technical field [0001] The present invention relates to a pharmaceutical composition, in particular to a preparation method of an anti-osteoporosis pharmaceutical composition. Background technique [0002] With the aging of the world's population, the incidence of osteoporosis is increasing year by year. The bone pain and fractures caused by osteoporosis directly affect the quality of life of middle-aged and elderly people, and have become serious social problems. Human bones are constantly undergoing remodeling through continuous bone formation and bone resorption, which is called bone remodeling. Osteogenesis and bone resorption maintain a good balance when young, but as we age, the balance tends to bone resorption due to various reasons, and if this state continues for a long time, the bone tissue becomes brittle, leading to osteoporosis Diseases, fractures and other bone diseases. [0003] A variety of drugs and methods for the prevention and treatment of osteoporosis ...

Claims

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Application Information

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IPC IPC(8): A61K36/804A61P19/10
Inventor 邢秋苓
Owner 邢秋苓
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