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Inhibitor of corneal epithelial cell death characterized by a combination of hyaluronic acid and flavin adenine dinucleotide

A corneal epithelial cell and flavin adenine technology is applied in the field of an inhibitor of corneal epithelial cell death which is characterized by a combination of hyaluronic acid and flavin adenine dinucleotide, and can solve the problem of undocumented corneal epithelial cells. Cell death, uninhibited death, unspecified corneal epithelial cell death, etc.

Inactive Publication Date: 2016-01-20
SANTEN PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The above-mentioned Non-Patent Document 1 discloses that hyaluronic acid and the like suppress the increase of reactive oxygen species production in corneal epithelial cells and the activation of Caspase-3 / 7 induced by UV-B irradiation, but it is not actually described whether hyaluronic acid and the like suppress the corneal epithelial cells induced by UV-B irradiation. corneal epithelial cell death
In addition, it is described in the above-mentioned Non-Patent Document 3 that hyaluronic acid and the like do not inhibit the cell death of corneal epithelial cells induced by desiccation.
[0006] Furthermore, it is not clear whether FAD actually inhibits the cell death of corneal epithelial cells induced by ultraviolet irradiation.
In addition, Japanese Patent Application Laid-Open No. 11-263729 (Patent Document 1) describes that FAD and the like have a lacrimal secretion-promoting effect, but it is not clear whether it directly inhibits the cell death of corneal epithelial cells induced by desiccation.

Method used

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  • Inhibitor of corneal epithelial cell death characterized by a combination of hyaluronic acid and flavin adenine dinucleotide
  • Inhibitor of corneal epithelial cell death characterized by a combination of hyaluronic acid and flavin adenine dinucleotide
  • Inhibitor of corneal epithelial cell death characterized by a combination of hyaluronic acid and flavin adenine dinucleotide

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Experimental program
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Embodiment

[0088] [Pharmacological Test 1]

[0089] Before ultraviolet (UV-B) irradiation, corneal epithelial cells were treated with the agent of the present invention, and in this case, whether the decrease in the number of living cells caused by ultraviolet irradiation was suppressed was evaluated.

test approach ）

[0091] SV40 immortalized human corneal epithelial cells (HCE-T: RIKEN, BioResourceCenter, CellNo.: RCB2280, the same as in Examples) were seeded in 96-well plates (1×10 4 cells / well), cultured in DMEM / F-12 medium containing 10% FBS for 1 day. The next day, replace the DMEM / F-12 medium containing 10% FBS with PBS containing 0.1% (w / v) sodium hyaluronate, 0.05% (w / v) flavin adenine dinucleoside PBS containing 0.1% (w / v) sodium hyaluronate and 0.05% (w / v) flavin adenine dinucleotide disodium salt, or PBS without the test substance ( Hereinafter, they are also referred to as "0.1% HA group", "0.05% FAD group", "0.1% HA / 0.05% FAD group" or "matrix group", respectively). Afterwards, the corneal epithelial cells were irradiated with UV-B (80mJ / cm 2 ) for about 1 minute. After the culture medium of each group was replaced with DMEM / F-12 medium, cultured at 37°C for 24 hours, thereafter, CellTiter96 (registered trademark) AQueous One Solution Cell Proliferation Assay (Aqueous One So...

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Abstract

A combination of hyaluronic acid or a salt thereof, and flavin adenine dinucleotide or a salt thereof can be used as a combination of corneal epithelial cells induced by ultraviolet irradiation or desiccation, and thus can be used as a and / or inhibitors of desiccation-induced corneal epithelial cell death.

Description

technical field [0001] The present invention relates to an inhibitor of corneal epithelial cell death induced by ultraviolet irradiation and / or desiccation, said inhibitor is characterized in that hyaluronic acid or its salt (hereinafter, these are also collectively referred to as "hyaluronic acid, etc.") and flavin adenine dinucleotide (FAD: FlavinAdenineDinucleotide) or its salt (hereinafter, these are also collectively referred to as "FAD, etc.") . The present invention also relates to a method for inhibiting corneal epithelial cell death, comprising the step of combining and administering to a patient a pharmaceutically effective amount of hyaluronic acid, etc., and FAD, etc. or dry induced. In addition, the present invention also relates to a use of a combination of hyaluronic acid and the like and FAD and the like for inhibiting corneal epithelial cell death induced by ultraviolet irradiation and / or drying. Furthermore, the present invention also relates to a use of a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/728A61K9/06A61K9/08A61K31/7084A61P27/02A61P43/00
CPCA61K9/0048A61K9/06A61K31/7084A61K31/728A61K47/06A61P27/02A61P43/00A61K2300/00
Inventor 阪元明日香中村雅胤
Owner SANTEN PHARMA CO LTD