Inhibitor of corneal epithelial cell death containing flavin adenine dinucleotide or its salt as an active ingredient
A technology of corneal epithelial cells and flavin adenine, applied in the field of inhibiting corneal epithelial cell death, application of inhibitors, and inhibitors of corneal epithelial cell death, which can solve ambiguous problems
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[0063] [pharmacological test]
[0064] Before ultraviolet (UV-B) irradiation, corneal epithelial cells were treated with FAD, and in this case, it was evaluated whether the decrease in the number of viable cells by ultraviolet irradiation was suppressed.
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[0066] SV40 immortalized human corneal epithelial cells (HCE-T: RIKEN, BioResourceCenter, CellNo.: RCB2280) were seeded in 96-well plates (1×104 / well), and cultured in DMEM / F-12 containing 10% FBS cultured for 1 day. On the next day, replace the DMEM / F-12 medium containing 10% FBS with PBS containing 0.0125% (w / v) or 0.05% (w / v) flavin adenine dinucleotide disodium salt, PBS containing 0.5% (w / v) sodium chondroitin sulfate (Sodiumchondroitinsulfateester), or PBS, and incubated at 37°C for 60 minutes (hereinafter, also referred to as "0.0125% FAD group", "0.05% FAD group", " 0.5%CS group" or "matrix group"). After that, UV-B irradiation (80 mJ / cm 2 ) was applied to the corneal epithelial cells for about 1 minute. After replacing the medium of each group with DMEM / F-12 medium containing 10% FBS again, culture at 37°C for 24 hours, after that, use CellTiter96 (registered trademark) AQueous single solution cell proliferation assay kit (AqueousOneSolutionCellProliferationAssay) (...
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