Detection step for reducing IPEC-J2 cell apoptosis through interaction between saccharomyces cerevisiae and DON and application thereof
A technology of IPEC-J2 and Saccharomyces cerevisiae, which is applied in the field of detection steps for the interaction between Saccharomyces cerevisiae and DON to reduce the apoptosis of IPEC-J2 cells, can solve the problems of reducing the detection steps of IPEC-J2 cell apoptosis, and achieve the reduction of apoptosis rate and necrosis rate, reduce the release of LDH, and increase the effect of proliferation rate
Active Publication Date: 2018-11-23
河南德邻生物制品有限公司 +1
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[0007] The invention proposes the detection steps and application of the interaction between Saccharomyces cerevisiae and DON to reduce the apoptosis of IPEC-J2 cells, and solves the problem of using Saccharomyces cerevisiae to repair damage to pig intestinal epithelial cells
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[0037] The technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0038] 1 Materials and methods
[0039] 1.1 Test material
[0040] Pig intestinal epithelial cells (IPEC-J2): donated by the Henan Provincial Key Research Laboratory of Animal-derived Food Safety, Henan Agricultural University. Saccharomyces cerevisiae is the strain preserved in this laboratory.
[0041] 1.1.1 Reagents
[0042]DMEM / F-12 culture medium (Hyclone, USA); fetal bovine serum (Fetal Bovine Serum, FBS) (Hangzhou Sijiqing Bioengineering Materials Co., Ltd.); penicillin and streptomyci...
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The invention discloses a detection step for reducing IPEC-J2 cell apoptosis through interaction between saccharomyces cerevisiae and DON and application thereof and aims to solve the problem that thesaccharomyces cerevisiae is used for repairing the damage of epithelial cells of pig intestines. Application of interaction between the saccharomyces cerevisiae and the DON (deoxynivalenol) in reduction of IPEC-J2 cell apoptosis can achieve an effect of alleviating inflammation and necrosis of the epithelial cells of the pig intestines induced by the DON. According to the detection step of reducing IPEC-J2 cell apoptosis through interaction between the saccharomyces cerevisiae and the DON and the application thereof, the saccharomyces cerevisiae (MOI=1) and the DON are co-cultured, so that the cell proliferation is accelerated to a certain extent; the damage of the DON to the epithelial cells of the pig intestines can be alleviated, and the integrity of an intestinal cell membrane is protected; when the concentration of the DON is 1.2 mu g / mL; through addition of the saccharomyces cerevisiae, the proliferation rate of the epithelial cells of the pig intestines and expression quantityof IL-10 anti-inflammatory cytokine can be obviously increased; the apoptosis rate and the necrosis rate of the cells can be obviously reduced; the saccharomyces cerevisiae has a certain alleviation effect on cytopathy induced by the DON.
Description
technical field [0001] The invention relates to the field of molecular biology, in particular to a detection step for reducing IPEC-J2 cell apoptosis through the interaction between Saccharomyces cerevisiae and DON and its application. Background technique [0002] Vomotoxin, also known as deoxynivalenol (DON), is one of the most common mycotoxins in grains and foods worldwide. A series of poisoning symptoms have been caused by humans and animals ingesting food containing DON. [0003] Porcine intestinal epithelial cells (IPEC-J2) are the first physical barrier against external stimuli, and DON is the first recognized toxin that has pro-inflammatory and immunomodulatory effects on intestinal epithelial cells, so this test aims to screen out The DON-degrading bacterial strain was constructed to construct the DON-IPEC-J2 cell model, and the degrading effect of the screened microorganisms on DON and the alleviation of the toxin's effect on cell proliferation were studied. [...
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IPC IPC(8): C12Q1/02C12N1/18C12R1/865
CPCG01N33/5014C12N1/18C12Q1/02G01N2333/395C12N2503/00
Inventor 尹清强刘洋常娟王平党晓伟黄玮玮刘超齐朱群李茂龙宋安东李庆华
Owner 河南德邻生物制品有限公司