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Inhibitor for corneal epithelial cell death, inhibitor characterized by combining hyaluronic acid and flavin adenine dinucleotide

A corneal epithelial cell, flavin adenine technology, applied in the field of group, can solve the problems of undocumented corneal epithelial cell death, uninhibited death, unspecified corneal epithelial cell death, etc.

Inactive Publication Date: 2014-05-28
SANTEN PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The above-mentioned Non-Patent Document 1 discloses that hyaluronic acid and the like suppress the increase of reactive oxygen species production in corneal epithelial cells and the activation of Caspase-3 / 7 induced by UV-B irradiation, but it is not actually described whether hyaluronic acid and the like suppress the corneal epithelial cells induced by UV-B irradiation. corneal epithelial cell death
In addition, it is described in the above-mentioned Non-Patent Document 3 that hyaluronic acid and the like do not inhibit the cell death of corneal epithelial cells induced by desiccation.
[0006] Furthermore, it is not clear whether FAD actually inhibits the cell death of corneal epithelial cells induced by ultraviolet irradiation.
In addition, Japanese Patent Application Laid-Open No. 11-263729 (Patent Document 1) describes that FAD and the like have a lacrimal secretion-promoting effect, but it is not clear whether it directly inhibits the cell death of corneal epithelial cells induced by desiccation.

Method used

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  • Inhibitor for corneal epithelial cell death, inhibitor characterized by combining hyaluronic acid and flavin adenine dinucleotide
  • Inhibitor for corneal epithelial cell death, inhibitor characterized by combining hyaluronic acid and flavin adenine dinucleotide
  • Inhibitor for corneal epithelial cell death, inhibitor characterized by combining hyaluronic acid and flavin adenine dinucleotide

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Experimental program
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Embodiment

[0088] [Pharmacological Test 1]

[0089] Before ultraviolet (UV-B) irradiation, corneal epithelial cells were treated with the agent of the present invention. In this case, it was evaluated whether the decrease in the number of viable cells caused by ultraviolet irradiation was suppressed.

test approach ）

[0091] SV40 immortalized human corneal epithelial cells (HCE-T: Institute of Physics and Chemistry, BioResource Center, CellNo.: RCB2280, the same in the examples below) were seeded on a 96-well plate (1×10 4 Pcs / well), cultured in DMEM / F-12 medium containing 10% FBS for 1 day. On the second day, replace the DMEM / F-12 medium containing 10% FBS with PBS containing 0.1% (w / v) sodium hyaluronate and 0.05% (w / v) flavin adenine dinucleoside Acid disodium salt, PBS containing 0.1% (w / v) sodium hyaluronate and 0.05% (w / v) flavin adenine dinucleotide disodium salt, or PBS containing no test substance ( Hereinafter, they are also referred to as “0.1% HA group”, “0.05% FAD group”, “0.1% HA / 0.05% FAD group” or “matrix group”). After that, UV-B irradiation (80mJ / cm 2 ) About 1 minute. After replacing the medium of each group with DMEM / F-12 medium, incubate at 37°C for 24 hours, and then use CellTiter96 (registered trademark) AQueous Single Solution Cell Proliferation Assay (Promega Compa...

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Abstract

An inhibitor for corneal epithelial cell death induced by ultraviolet irradiation and / or dryness, the inhibitor dramatically inhibits the decline of the number of living cells in corneal epithelial cells induced by ultraviolet irradiation or dryness as a result of combining hyaluronic acid or a salt thereof with flavin adenine dinucleotide or a salt thereof.

Description

Technical field [0001] The present invention relates to an inhibitor, which is an inhibitor of corneal epithelial cell death. The corneal epithelial cell death is induced by ultraviolet irradiation and / or drying. The inhibitor is characterized in that it is hyaluronic acid or its Salt (hereinafter, these are also collectively referred to as "hyaluronic acid, etc.") and flavin adenine dinucleotide (FAD: Flavin Adenine Dinucleotide) or its salt (hereinafter, these are also collectively referred to as "FAD etc.") Into. The present invention also relates to a method, which is a method for inhibiting corneal epithelial cell death, including the step of combining and administering to a patient a pharmaceutically effective amount of hyaluronic acid, etc., FAD, etc., the corneal epithelial cell death is irradiated by ultraviolet rays and / Or induced by drying. In addition, the present invention also relates to an application, which is an application of a combination of hyaluronic acid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/728A61K9/06A61K9/08A61K31/7084A61P27/02A61P43/00
CPCA61K9/06A61K31/7084A61K9/0048A61K47/06A61K31/728A61P27/02A61P43/00A61K2300/00
Inventor 阪元明日香中村雅胤
Owner SANTEN PHARMA CO LTD