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Application of sirt-1 protein in diagnosis and prediction of metastatic products of osteosarcoma

An osteosarcoma and bone diagnosis technology, applied in the application field of protein, can solve the problems such as no related research on Sirt1 reported in literature, no related research on Sirt1 expression and osteosarcoma metastasis, etc.

Inactive Publication Date: 2016-06-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the biological function of Sirt1 has been gradually elucidated, there is no literature report on the correlation between Sirt1 and osteosarcoma, let alone the correlation between Sirt1 expression and osteosarcoma metastasis.

Method used

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  • Application of sirt-1 protein in diagnosis and prediction of metastatic products of osteosarcoma
  • Application of sirt-1 protein in diagnosis and prediction of metastatic products of osteosarcoma
  • Application of sirt-1 protein in diagnosis and prediction of metastatic products of osteosarcoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 33 pairs of osteosarcoma clinical samples and paracancerous tissues were selected, fixed with 10% formaldehyde solution for 7 days, and then paraffin-embedded tissue blocks were prepared. Paraffin sections with a thickness of 8 microns were prepared from the samples, and the expression of Sirt-1 protein was detected by immunohistochemical method, and the expression of Sirt-1 protein was observed under an upright microscope. like figure 1 As shown, the expression of Sirt-1 protein in cancer tissues was significantly higher than that in paracancerous tissues. The statistics of Sirt-1 protein expression in these 33 pairs of osteosarcoma clinical samples and adjacent tissues showed that 15 cases were strongly positive in cancer tissues, 14 cases were positive, 3 cases were weakly positive, and 1 case was negative; In the paracancerous tissues, 1 case was strongly positive, 3 cases were positive, 11 cases were weakly positive, and 18 cases were negative ( figure 2 ).

Embodiment 2

[0026] The 33 cases of osteosarcoma in the aforementioned embodiment 1 were retrospectively investigated for 3 years, and 22 cases had detailed follow-up records, so the relationship between Sirt-1 protein expression and the prognosis of osteosarcoma patients was analyzed in these 22 cases of samples . It was found that the stronger the expression of Sirt-1, the higher the metastasis rate of osteosarcoma patients (Table 1).

[0027] Table 1 Correlation of Sirt-1 protein level with lung metastasis and 3-year survival rate in osteosarcoma

[0028]

Embodiment 3

[0030] Seven primary osteosarcoma cells cultured in vitro were selected, and the expression of Sirt-1 protein was detected by Western blotting, and Actin was used as an internal reference. image 3 As shown in A, there are differences in the expression levels of Sirt-1 protein in different primary osteosarcoma cells. The invasion and migration ability of primary osteosarcoma cells was detected by Transwell method. like image 3 As shown in B, in general, the high expression of Sirt-1 protein in primary osteosarcoma cells is positively correlated with its invasion and movement ability in vitro. image 3 The darker the color in A, the stronger the expression of Sirt-1 in the cell. image 3 The darker the color of B, the stronger the invasion ability, image 3 A and image 3 B results are positively correlated.

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Abstract

The invention discloses Sirt-1 protein applied in diagnosis and prediction of an osteosarcoma transfer product; the Sirt-1 protein can serve as a mark molecule of osteosarcoma diagnosis, thereby improving osteosarcoma diagnosis and osteosarcoma transfer prediction accuracy. The product using immunodetection to diagnosis osteosarcoma and predict osteosarcoma transfer comprises antibody specifically binding to the Sirt-1 protein. The product diagnosing osteosarcoma and predicting osteosarcoma transfer comprises the product using RT-PCR, real time quantification PCR, immunodetection, original position hybridization or gene chip to diagnosis osteosarcoma and predict osteosarcoma transfer. The Sirt-1 protein can serve as the singularity sign protein of diagnosis and transfer prediction, so the osteosarcoma diagnosis can be more accurate and fast.

Description

technical field [0001] The present invention relates to the application of a protein, in particular to the application of the Sirt-1 protein in the diagnosis and prediction of osteosarcoma metastatic products. Background technique [0002] Osteosarcoma is one of the most common malignant bone-forming tumors. Epidemiological studies have found that the disease occurs more frequently in adolescents aged 15-25, and the incidence rate is higher in males than in females. The predilection site of osteosarcoma is the metaphysis of long tubular bones. The tumor has a high degree of malignancy and a very poor prognosis. The 5-year survival rate of patients after simple amputation is less than 20%. In addition, another notable feature of this tumor is lung metastasis. Early, when osteosarcoma was diagnosed clinically, most of them had already developed micrometastases in the lung. Therefore, the effective diagnosis of osteosarcoma, especially the development of technologies to predic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/532
CPCG01N33/57484G01N33/6893G01N2800/50
Inventor 张宁叶招明应美丹曹戟王强刘兵
Owner ZHEJIANG UNIV