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Streptococcus mutans AMC metabolic recovery strain based on LuxS defect

A technology of Streptococcus mutans and restorer strains, applied in the field of Streptococcus mutans AMC metabolic restorer strains and its construction, can solve the problems of weakened influence, changes in gene transcription or changes in physiological characteristics that have not been restored, and achieve the effect of overcoming research obstacles

Inactive Publication Date: 2014-06-18
SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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AI Technical Summary

Problems solved by technology

However, in recent years, many studies have found that many gene transcription changes or physiological property changes caused by LuxS deletion have not been restored by adding exogenous AI-2.
Therefore, the influence of density sensing mediated by LuxS / AI-2 in the regulation of LuxS is gradually weakened

Method used

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  • Streptococcus mutans AMC metabolic recovery strain based on LuxS defect
  • Streptococcus mutans AMC metabolic recovery strain based on LuxS defect
  • Streptococcus mutans AMC metabolic recovery strain based on LuxS defect

Examples

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Embodiment Construction

[0026] Below in conjunction with specific embodiment, further illustrate the present invention. The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. The experimental method that does not indicate specific condition in the following examples is generally according to conventional conditions, such as Sambrook et al., molecular cloning: the conditions described in the laboratory handbook (New York: Cold Spring Harbor Laboratory Press, 1989), or according to the manufacture conditions recommended by the manufacturer.

[0027] 1. Strains, plasmids and main reagents

[0028] Strain: Streptococcus mutans UA159 (provided by Shanghai Institute of ...

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Abstract

The invention discloses a streptococcus mutans AMC metabolic recovery strain based on LuxS defect, wherein AMC metabolic recovery strain is StreptococcusmutansKO-SUA159:: delta luxS, pIB-sahH and has a preservation number of CCTCCM2013699. The LuxS-mediated metabolism is separated from density induction by heterologous expression of SahH in a Streptococcus mutans stain with LuxS defect, the obstacles to research on the LuxS mechanism, caused by dual-defect effect of the deletion of LuxS, is effectively overcome, and an effective molecular biology tool and experimental control are provided for the discuss about the regulation mechanism of Streptococcus mutans LuxS.

Description

technical field [0001] The present invention relates to the research field of microbial density sensing mechanism, more specifically, relates to LuxS-deficient Streptococcus mutans AMC metabolic recovery strain and its construction. Background technique [0002] Biofilm is the existence form of most microorganisms in nature, and its formation can greatly enhance the survival ability of microorganisms and the ability to resist from the environment, hosts and antibacterial drugs. Therefore, biofilm formation has become the main pathogenic factor of many chronic intractable infectious diseases. Density sensing plays an important role in the formation, development and maturation of biofilms. Quorum sensing (QS) is a quorum communication mechanism of bacteria, that is, bacteria synthesize, secrete and perceive signal molecules in the environment to regulate the expression of some of their own genes. Among the various known signaling molecules, autoinducer-2 (autoinducer-2, AI-2)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/21C12N15/74C12R1/46
Inventor 黄正蔚王玉霞
Owner SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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