Method for measuring stable hemoglobin a1c using liquid chromatography, and method for simultaneous measurement of stable hemoglobin a1c and abnormal hemoglobin using liquid chromatography

A technology of hemoglobin and liquid chromatography, which is applied in the field of determination of stable hemoglobin A1c, can solve problems such as changes in measured values, increased coating thickness, and clogging

Inactive Publication Date: 2014-06-18
SEKISUI MEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the coating treatment using silicones has the following disadvantages: Depending on the coating conditions, the coating thickness increases and clogging is likely to occur, the pressure may increase due to continuous use for a long time, and the separation performance deteriorates when the measurement time is shortened. ,Wait
These shortcomings, especially in the case of high-pressure measurement systems such as Patent Document 1, are significantly reflected
[0006] In addition, when stable hemoglobin A1c and abnormal hemoglobin are measured at the same time, the measured value changes greatly due to the increase in the number of separation peaks and the decrease in separation performance.

Method used

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  • Method for measuring stable hemoglobin a1c using liquid chromatography, and method for simultaneous measurement of stable hemoglobin a1c and abnormal hemoglobin using liquid chromatography
  • Method for measuring stable hemoglobin a1c using liquid chromatography, and method for simultaneous measurement of stable hemoglobin a1c and abnormal hemoglobin using liquid chromatography
  • Method for measuring stable hemoglobin a1c using liquid chromatography, and method for simultaneous measurement of stable hemoglobin a1c and abnormal hemoglobin using liquid chromatography

Examples

Experimental program
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Effect test

manufacture example 1）

[0124] In Production Examples 1 to 3, column packing materials for measuring hemoglobins having different average particle diameter values ​​were prepared.

[0125] 150 g of tetraethylene glycol dimethacrylate (cross-linkable monomer, manufactured by Shin-Nakamura Chemical Industry Co., Ltd.), 140 g of triethylene glycol dimethacrylate (cross-linkable monomer, manufactured by Shin-Nakamura Chemical Industry Co., Ltd.) , and 2-hydroxy-1,3-dimethacryloyloxypropane (crosslinkable monomer, manufactured by Shin-Nakamura Chemical Industry Co., Ltd.) 60 g of monomer mixture, mix and dissolve benzene peroxide as a polymerization initiator Formyl (manufactured by KISHIDA Chemical Co., Ltd.) 1.0 g was dispersed in 2000 mL of a 5% by weight polyvinyl alcohol (manufactured by Nippon Synthetic Chemicals Co., Ltd., “Gohsenol GH-20”) aqueous solution. While stirring the reaction system at a speed of 350 rpm, it was heated to 80° C. in a nitrogen atmosphere to perform a polymerization reactio...

manufacture example 2）

[0127] A column packing was obtained in the same manner as in Production Example 1 except that the stirring conditions of the reaction system in Production Example 1 were changed to 300 rpm. As a result of measuring the average particle diameter value in the same manner as in Production Example 1, it was 6.4 μm.

manufacture example 3）

[0129] A column packing was obtained in the same manner as in Production Example 1 except that the stirring conditions of the reaction system in Production Example 1 were changed to 250 rpm. The average particle diameter was measured in the same manner as in Production Example 1, and it was 9.5 μm.

[0130] Table 1 shows the average particle diameters of the column fillers obtained in Production Examples 1 to 3 above.

[0131] [Table 1]

[0132]

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PUM

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Abstract

The purpose of the present invention is to provide a method for measuring stable hemoglobin A1c in a short time and in a highly reproducible manner, using liquid chromatography. The present invention is a method for measuring stable hemoglobin A1c using liquid chromatography, wherein a filter, the surface of which has been treated with a solution containing 1-50 weight% of silicone oil or a solution containing 1-50 weight% of silicone resin, is placed on the channel of a liquid chromatograph and the pressure generated on the measurement system of the liquid chromatograph is set to 9.8 103 Pa to 19.6 105 Pa.

Description

technical field [0001] The present invention relates to a method for measuring stable hemoglobin A1c using liquid chromatography. In addition, the present invention also relates to a method for simultaneously measuring stable hemoglobin A1c and abnormal hemoglobin by liquid chromatography. Background technique [0002] The measurement of hemoglobin by liquid chromatography can measure the stable hemoglobin A1c with high precision in a short time, so it is especially used in the control of the hemoglobin A1c value of diabetic patients. This application requires that the CV value (%) of the hemoglobin A1c value in the reproducibility test be about 1% or less. [0003] A general method for measuring stable hemoglobin A1c with good reproducibility in a short period of time by liquid chromatography is to make the particle diameter of column packing particles small and uniform. However, if the particle size of the column filler particles is reduced, the pressure value generated ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/32G01N30/56
CPCG01N2030/328G01N33/721G01N30/52G01N2030/527G01N30/32G01N30/14G01N2030/567G01N2030/8831G01N2030/525
Inventor 大石和之
Owner SEKISUI MEDICAL CO LTD
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