Method of synchronously extracting polysaccharides and proteins from subcritical water

A subcritical water and protein technology, which is applied in the preparation method of peptides, chemical instruments and methods, organic chemistry, etc., can solve problems such as patents that have not been reported, and achieve the effects of simple production process, improved dissolution rate, and easy industrialization

Inactive Publication Date: 2014-06-25
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many research reports on the use of SWE to pretreat environmental samples, sewage and soil for analysis. In recent years, subcritical water has been used to extract natural active ingredients such as vol

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take 10g of degreased and dried lotus seed powder and put it into the extraction kettle. For the first extraction, pump deionized water with a pH of 7.0 according to the ratio of water to material 40:1, control the extraction pressure at 5MPa, the extraction temperature at 140°C, and the extraction time at After 16 min, the extract was released. Continue to pump deionized water with a water-to-material ratio of 30:1 and pH 5.0 into the filter residue, control the extraction pressure to remain constant, extract the temperature at 160°C, and extract for 10 minutes. Cool and separate the extract. Repeat the above operation once, and combine all the extracts to obtain the extracts of polysaccharides and proteins. The extract was filtered through a 200-mesh industrial filter cloth, concentrated to 1 / 10 of the original volume, freeze-dried polysaccharide and protein extract powder, the total yield of polysaccharide and protein was 67.83%, and the yield of polysaccharide and p...

Embodiment 2

[0026] Take 10 g of degreased and dried Cordyceps oryzae and put it into the extraction kettle. For the first extraction, pump deionized water with a pH of 9.0 according to the water-to-material ratio of 20:1. Control the pressure in the extraction kettle to 8 MPa and the temperature to 100 °C. After extracting for 17 min, release the extract. Continue to pump deionized water with a water-material ratio of 20:1 and a pH of 6.0 into the filter residue, control the extraction pressure to remain constant, extract the temperature at 180°C, and extract for 10 min, cool and separate the extract. Repeat the above operation once, and combine all the extracts to obtain the extracts of polysaccharides and proteins. The extract was filtered through a 200-mesh industrial filter cloth, and the filtrate was vacuum-dried to obtain polysaccharide and protein extract powders. The total yield of polysaccharide and protein was 31.19%, of which the polysaccharide and protein yields were 10.84% ​​...

Embodiment 3

[0028] Take 10 g of degreased and dried rice bran and put it into the extraction kettle. For the first extraction, pump deionized water with a pH of 9.0 according to the water-material ratio of 40:1, control the pressure in the extraction kettle to 8 MPa, and the temperature to 140°C. After 20 min, release the extract. Continue to pump deionized water with a water-to-material ratio of 30:1 and pH 5.0 into the filter residue, control the extraction pressure to remain constant, extract the temperature at 160°C, and extract for 30 min. Cool and separate the extract. Repeat the above operation once, and combine all the extracts to obtain the extracts of polysaccharides and proteins. The extract was filtered through a 200-mesh industrial filter cloth, and the filtrate was vacuum-dried to obtain polysaccharide and protein extract powders. The total yield of polysaccharide and protein was 27.52%, of which the polysaccharide and protein yields were 8.81% and 18.71%, respectively.

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Abstract

The invention discloses a method of synchronously extracting polysaccharides and proteins from subcritical water, and belongs to the technical field of separation active ingredients of natural products. The process comprises production processes of material pre-treating, synchronous extracting, filtering, drying, sterilizing and packaging, so that products rich in polysaccharides and proteins are obtained. The method disclosed by the invention can be used for simultaneously extracting two nutrient substances which are polysaccharides and proteins by controlling extracting parameters. Dried powder has the characteristics of being natural, nutrient, convenient to mix, and the like, can be used as raw materials of products such as food, beverages, health care products and medicines, or additives or dietary supplements, and the like. The production process is clean, environment-friendly, simple in technology, and easy for realization of industrialization.

Description

technical field [0001] The invention relates to a method for extracting active polysaccharides and proteins, in particular to a method for synchronously extracting polysaccharides and proteins with subcritical water, and belongs to the technical field of separation of active components of natural products. Background technique [0002] Polysaccharides and proteins are a class of natural macromolecules derived from higher plants, animals and microorganisms. Protein is an important part of the structure of organisms, the main raw material for tissue renewal and repair, and has a variety of physiological functions. Protein research has always been a research hotspot among scholars in the world. Polysaccharides are considered to be the most urgent biological macromolecules after proteins. In addition to being used as energy storage or structural materials in organisms, polysaccharides also play important roles in cell recognition, signal transduction, and intercellular materia...

Claims

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Application Information

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IPC IPC(8): C08B37/00C07K1/14
Inventor 张海晖段玉清柯佳佳杨文雅朱婷华王海刚
Owner JIANGSU UNIV
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