Method for producing compound bacteria for water environment improvement

A production method and technology of composite bacteria, applied in the field of applied biology, can solve problems such as slow effect, inability of microorganisms to produce enzymes, prominent eutrophication of water sources, etc., and achieve the effect of improved effect, strong pertinence and combination ability

Inactive Publication Date: 2014-07-02
都江堰惠农生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] As more than 90% of drinking water sources: lakes, reservoirs and rivers, the main manifestations are: 1. The problem of eutrophication in the water source area is prominent, and the total nitrogen and total phosphorus exceed the standard; 2. The water source itself produces a lot of debris from the metabolism of animals and plants, which cannot be used traditional methods of eradication
3. The increase of pathogenic microorganisms in the water body
4. The eutrophication of the water body environment is serious, resulting in a large number of algae blooms
[0005] In recent years, microbial preparations have been used in water environment improvement, but there are many problems, such as some microorganisms cannot produce enzymes, but simply absorb small molecule nutrients in water bodies, and cannot effectively promote the material circulation of water body environments. slower

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0044] 1) Activate the freeze-dried strains or the strains stored at 4°C on a slope. Inoculate Bacillus subtilis, Bacillus licheniformis, and Alcaligenes denitrifica into nutrient broth agar medium for slope activation; inoculate actinomycetes into starch agar medium for slope activation; inoculate photosynthetic bacteria into photosynthetic bacteria solid medium ramp activation.

[0045]2) Preparation of primary seed liquid: Pick typical colonies from the activated agar plate, inoculate Bacillus subtilis Bacillus licheniformis denitrifying Alcaligenes into sterile nutrient broth with pH 7.0, actinomycetes Inoculate in sterilized starch liquid medium, 35°C, 150rpm, culture overnight; inoculate photosynthetic bacteria into photosynthetic bacteria liquid medium, 35°C, light culture for 4 days, light intensity 1500Lx: obtain first-class seeds of each strain liquid.

[0046] 3) Fermentation of secondary seed liquid: respectively insert the Bacillus subtilis, Bacillus licheniform...

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Abstract

The invention provides a method for producing compound bacteria for water environment improvement. The compound bacteria comprise the following strains in percentage by weight: 30-40 percent of bacillus subtilis, 30-40 percent of bacillus licheniformis, 10-20 percent of actinomycetes, 10-20 percent of denitrifying bacteria and 10-20 percent of photosynthetic bacteria, totally 100 percent.

Description

technical field [0001] The invention belongs to the field of applied biotechnology, and relates to a microbial composite bacterium for water environment improvement and a production method thereof. Background technique [0002] Water is the source of life, and human survival activities cannot do without water. Water safety is related to the health of the people and sustainable economic and social development, and is the top priority of environmental protection. The World Health Organization (WHO) survey shows that 80% of diseases and 50% of child deaths in the world are related to poor drinking water quality. There are more than 50 diseases caused by poor drinking water quality. [0003] As more than 90% of drinking water sources: lakes, reservoirs and rivers, the main manifestations are: 1. The problem of eutrophication in the water source area is prominent, and the total nitrogen and total phosphorus exceed the standard; 2. The water source itself produces a lot of debri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/125C12R1/10
Inventor 李朝均
Owner 都江堰惠农生物技术有限责任公司
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