Method for producing compound bacteria for water environment improvement
A production method and technology of composite bacteria, applied in the field of applied biology, can solve problems such as slow effect, inability of microorganisms to produce enzymes, prominent eutrophication of water sources, etc., and achieve the effect of improved effect, strong pertinence and combination ability
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[0044] 1) Activate the freeze-dried strains or the strains stored at 4°C on a slope. Inoculate Bacillus subtilis, Bacillus licheniformis, and Alcaligenes denitrifica into nutrient broth agar medium for slope activation; inoculate actinomycetes into starch agar medium for slope activation; inoculate photosynthetic bacteria into photosynthetic bacteria solid medium ramp activation.
[0045]2) Preparation of primary seed liquid: Pick typical colonies from the activated agar plate, inoculate Bacillus subtilis Bacillus licheniformis denitrifying Alcaligenes into sterile nutrient broth with pH 7.0, actinomycetes Inoculate in sterilized starch liquid medium, 35°C, 150rpm, culture overnight; inoculate photosynthetic bacteria into photosynthetic bacteria liquid medium, 35°C, light culture for 4 days, light intensity 1500Lx: obtain first-class seeds of each strain liquid.
[0046] 3) Fermentation of secondary seed liquid: respectively insert the Bacillus subtilis, Bacillus licheniform...
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