A kind of tissue engineering meniscus repair sheet and preparation method thereof

A technology of tissue engineering and meniscus, which is applied in the field of tissue engineering medical biomaterials, can solve the problems of inability to promote the fusion of materials and tissues, the difficulty of firm attachment of cells, and the lack of nutrition in joint synovial fluid, so as to achieve firm attachment and high inoculation rate. High, short inoculation time effect

Active Publication Date: 2016-04-27
西安博鸿生物技术有限公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

It has been reported in the literature that the tissue engineered meniscus prepared by articular chondrocytes combined with acellular meniscus scaffold material can be used for meniscus tear repair, but the structure of the acellular meniscus scaffold material is dense, and the cells are difficult to attach firmly. And it is easy to fall off after implanted in the body, and the synovial fluid has less nutrition, which cannot promote the fusion of the material and its own tissue.

Method used

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  • A kind of tissue engineering meniscus repair sheet and preparation method thereof
  • A kind of tissue engineering meniscus repair sheet and preparation method thereof
  • A kind of tissue engineering meniscus repair sheet and preparation method thereof

Examples

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example 1

[0031] The seed cells used in Example 1 were human bone marrow mesenchymal stem cells purchased from Beijing Yuhengfeng Technology Co., Ltd.

example 2

[0032] The seed cells used in example 2 are rabbit fibrochondrocytes, and the isolation and culture methods are referred to literature: Pan Xianfeng, Lin Yueqiu, Tang Xun etc. The cultivation and biological characteristics of rabbit meniscus fibrochondrocytes, Chinese Journal of Orthopedic Surgery, October 2000 Expect.

[0033] The ultraviolet laser drilling instrument in Example 1 is Wuhan Chutian Laser Co., Ltd. PSV-6001 type, and its ultraviolet wavelength is 355nm.

[0034] In Example 2, the instrument entrusted to Suzhou Delong Laser Co., Ltd. for drilling is DeepPioneer picosecond laser fine micromachining equipment.

[0035] The 5mL flat-bottomed centrifuge tube used in the example is the LXG-5 model produced by Jiangsu Haimen Bedford Experimental Equipment Company.

Embodiment 1

[0037] Step 1. Cultivation of human bone marrow mesenchymal stem cells: resuscitate purchased first-generation human bone marrow mesenchymal stem cells and inoculate them in human bone marrow mesenchymal stem cell culture medium, and culture them in a cell incubator. The first time after 3 days Change the medium, and then change the medium every other day. After 5 days, the cells reach more than 80% confluence, and then digest with 0.25% trypsin solution to obtain the second-generation human bone marrow mesenchymal stem cells for use; the human bone marrow mesenchymal stem cells cultured The base is α-MEN medium containing 10% fetal bovine serum (FBS);

[0038]Step 2. Preparation of Growth Factor Sustained Release Carrier: Preparation Method of Chitosan Microspheres References: Chen Yu, Liu Zhaoli, Wu Qinghui, etc. Preparation of Micron Chitosan Microspheres. Journal of Dalian University of Technology, Issue 03, 2012 , the particle size of the prepared chitosan microspheres wa...

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Abstract

A tissue engineered meniscus repair sheet and a preparation method thereof. The tissue engineered meniscus repair sheet prepared by the present invention includes a decellularized meniscus sheet, seed cells and growth factor slow-release carriers, wherein the two surfaces of the decellularized meniscus sheet are uniform Micro blind holes are distributed, and seed cells and slow-release growth factor carriers are compounded on both surfaces of the decellularized meniscus sheet. The micro-blind pores distributed on the decellularized meniscus sheet increase the area of ​​the scaffold material, which can be used as a storage pool for seed cells and slow-release carriers of growth factors, making them firmly attached and not easy to fall off; The carrier can release nutrients for a long time, improve the efficiency of cell expansion, maintain cell phenotype, inhibit cell aging, and promote the fusion of tissue engineering meniscus repair sheet and its own tissue. The prepared tissue engineered meniscus repair sheet has structural characteristics similar to natural meniscus, and can be used for repairing meniscus injury and inducing meniscus fibrocartilage tissue regeneration.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering medical biomaterials, in particular to a tissue engineering meniscus repair sheet with natural structure and function and a preparation method thereof. Background technique [0002] The menisci are two half-moon-shaped fibrocartilages located on the medial and lateral articular surfaces of the tibial plateau. They have the functions of stabilizing the knee joint, transmitting and distributing the load force of the knee joint, and promoting nutrition in the joint. Important organization of functions. Meniscal injuries are mostly caused by torsional external forces, causing joint pain, limited joint mobility, muscle atrophy, and walking inconvenience, which seriously affect the lives of patients. Meniscal injuries are one of the most common sports injuries of the knee. [0003] From the outside to the inside of the meniscus, the outer 10-20% is the red area, which is supplied by the inn...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/38A61L27/36A61L27/54A61L27/56A61L27/50A61L27/22A61L27/24A61L27/20
Inventor 张立娟刘影
Owner 西安博鸿生物技术有限公司
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