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Preparation method of drypetes congestiflora stem extract

A technology for extracts of drupe drupes and stems, which is applied in the field of preparation of drupe drupe stem extracts, can solve problems such as no research reports on chemical components and pharmacological activities, and achieves simple process flow and good anti-tumor activity. , the effect of clear chemical composition

Inactive Publication Date: 2014-07-16
HAINAN NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Drypetes congestiflora is distributed in the mountainous sparse forests of Hainan, Guangdong, Yunnan and other provinces. It is a new pseudo-species and is the main economic tree in Hainan. There are no research reports on its chemical composition and pharmacological activity at home and abroad.

Method used

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  • Preparation method of drypetes congestiflora stem extract
  • Preparation method of drypetes congestiflora stem extract

Examples

Experimental program
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Effect test

preparation example Construction

[0022] The preparation method steps of Drupe densiflora tree stem extract are as follows:

[0023] 1) Add 85-95% ethanol or methanol to the crushed Drupe densiflora (through a 20-80 mesh sieve), soak at room temperature, heat or ultrasonically extract, the extraction time is 60 minutes to 7 days, and the material liquid The ratio is 1:1~1:10;

[0024] 2) Filter the drupe densiflora medicinal material extract, recover under reduced pressure to obtain the total extract, add water to suspend, add water to suspend, sequentially extract with petroleum ether, chloroform, ethyl acetate and n-butanol, and recover under reduced pressure to obtain petroleum Ether part, chloroform part, ethyl acetate part and n-butanol part;

[0025] 3) Carry out repeated column chromatography on the part of petroleum ether that has been determined to have extensive anti-tumor activity: petroleum ether: ethyl acetate (20:1) silica gel column chromatography to obtain compounds (1), (3) and (10); Petrole...

example 1

[0028] The preparation of example one Drupe densiflora tree stem effective part

[0029] Drupe densiflora tree stems (20Kg), soaked in 95% ethanol at room temperature for 3 times, 7 days / time, combined the extracts, concentrated under reduced pressure to obtain the total extract (1.0Kg), added water to suspend and disperse, respectively used petroleum ether, Chloroform, ethyl acetate and n-butanol were extracted to obtain petroleum ether fraction (80g), chloroform fraction (120g), ethyl acetate fraction (15g) and n-butanol fraction (450g).

[0030] The petroleum ether part undergoes repeated column chromatography on silica gel and Sephdex LH-20 to obtain 3 triterpenoids, 7 sterols and 1 glyceride derivative, which are respectively petroleum ether: ethyl acetate Esters (20:1) silica gel column chromatography to obtain 30 mg of β-aryl resin (1), 47 mg of suberinone (3) and 356 mg of β-sitosterol (10); petroleum ether: acetone (20:1) followed by 17 mg of 3α-hydroxyoleanolic acid...

Embodiment 2

[0032] Example 2 Study on Antitumor Activity of Petroleum Ether Parts of Drupe densiflora

[0033] 1. Experimental method: Three tumor cell lines K562, SPCA-1, and SGC-7901 were used in RPMI-1640 medium containing 10% calf serum at 37°C and 5% CO 2 cultured in an incubator. The MTT method was used to carry out the cell proliferation inhibition test. The main operation was to take the tumor cell lines in the logarithmic growth phase, digest them with 0.25% trypsin, and prepare 5×10 RPMI-1640 culture medium with 10% newborn calf serum. 4 pcs / ml -1 The cell suspension was inoculated in a 96-well plate with 180 μL per well. At 37°C, 5% CO 2 Cultivate for 8-10 hours under saturated humidity conditions, and when they adhere to the wall, add sample solution prepared with PBS to each well so that the final concentrations of the samples are 1, 10, and 100 μg / mL, respectively. For each concentration, 3 wells were paralleled, and after 44 hours of continuous culture, 50 μL MTT (1 mg / ...

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Abstract

The invention discloses a preparation method of a drypetes congestiflora stem extract. The preparation method comprises the steps: adding a solvent in dried drypetes congestiflora stem powder for extracting; and performing filtration and vacuum concentration on an extraction solution to obtain a total extract, adding water for suspending, sequentially extracting by using petroleum ether, chloroform, ethyl acetate and normal butanol, and performing decompression recovering to respectively obtain a petroleum ether part, a chloroform part, an ethyl acetate part and a normal butanol part, wherein the petroleum ether part comprises three triterpene compounds, 7 sterols compounds and one glyceride derivative. The extract prepared by adopting the preparation method is high in yield, simple in process, low in cost, suitable for larger-scale preparation, and good in anti-tumor activity, and can be applied to medicines for preventing and treating tumors.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of effective components of natural plants, and relates to a preparation method of drupe densiflora stem extract. Background technique [0002] There are about 200 species of Euphorbiaceae (Drypetes) in the world, which are distributed in tropical and subtropical regions such as Asia, America, and central and western Africa. This genus of plants is a traditional medicinal material in African folk, mainly used to treat gonorrhea, malignant tumors, kala-azar, rheumatoid, bronchitis and other diseases, and has a long history of medicinal use. As a traditional drug for the treatment of malignant tumors, Drupeaceae plants have great potential for the development of anti-tumor activity research. [0003] The antitumor effect of traditional Chinese medicine has been widely concerned. Many clinical trials and experiments have proved that many traditional Chinese medicines have obvious e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00C07J9/00C07J21/00C07J75/00C07C69/30C07C67/48
Inventor 陈文豪陈光英宋小平惠阳
Owner HAINAN NORMAL UNIV
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