Method for inhibiting in-vivo BmNPV viral multiplication of silkworms

Abstract

The invention discloses a method for inhibiting in-vivo BmNPV viral multiplication of silkworms. The method comprises the following steps: firstly preparing nano-titanium dioxide into an additive solution with the concentration of 4.5-5.5 mg / L; spraying the solution on mulberry leaves according to the use dosage that 1.0L of solution is added to 100kg of mulberry leaves; feeding to the silkworms after the mulberry leaves are dried, so that the in-vivo BmNPV viral multiplication of the silkworms can be obviously inhibited. According to the method, the nano-titanium dioxide is fed to the silkworms to inhibit the in-vivo BmNPV viral multiplication, and thus a theoretical support can be provided for the preventing and curing of BmNPV disease of silkworms. The disclosed method is high in practicability, convenient in operation and obvious in effect, and is suitable for popularization and application in sericultural areas of our country.

Description

technical field

[0001] The invention relates to a method for inhibiting virus proliferation in silkworms, in particular to a method for inhibiting BmNPV virus proliferation in silkworms through the action of nanoparticles. Background technique

[0002] silkworm( Bombyx mori ) is an important economic insect, which has been domesticated and raised for more than 5700 years. Its silk production performance has been greatly improved, but its resistance to pathogens is weak. In recent years, the incidence of silkworm diseases has increased due to the overlap of silkworm breeding periods and incomplete disinfection.

[0003] Bombyx mori nuclear polyhedrosis virus (BmNPV) has the highest incidence rate among silkworm diseases. Because the virus is protected by a polyhedron shell outside the body, it exists for a long time and is difficult to disinfect; in addition, due to the long incubation period of the disease, once it breaks out, it will cause Sericulture farmers suffered hea...

Examples

Embodiment 1

[0019] Example 1: A method for inhibiting the proliferation of BmNPV in the silkworm

[0020] ⑴Materials and medicines:

[0021] The silkworm variety: Suxiu×Chunfeng, obtained the silkworm variety certificate issued by the Silkworm Variety Approval Committee of Jiangsu Province (Su Shensi 201001), which has the basic commonality of silkworm.

[0022] Drugs: nano titanium dioxide (TiO 2 ) is a product of Hangzhou Wanjing New Material Co., Ltd.

[0023] Other molecular biology reagents are products of Sangon Bioengineering (Shanghai) Co., Ltd.

[0024] ⑵The use of nano-titanium dioxide

[0025] Mix nano-titanium dioxide with water to prepare an additive solution with a concentration of 5.0 mg / L; fully mix the additive before use, and spray 1.0 L dose per 100 kg of mulberry leaves on mature leaves of various ages. The mulberry variety is Yu 711, After the ripe leaves were dried, silkworms were fed continuously for 3 days, and the amount of mulberry was the same as the usual a...

technical field

[0001] The invention relates to a method for inhibiting virus proliferation in silkworms, in particular to a method for inhibiting BmNPV virus proliferation in silkworms through the action of nanoparticles. Background technique

[0002] silkworm( Bombyx mori ) is an important economic insect, which has been domesticated and raised for more than 5700 years. Its silk production performance has been greatly improved, but its resistance to pathogens is weak. In recent years, the incidence of silkworm diseases has increased due to the overlap of silkworm breeding periods and incomplete disinfection.

[0003] Bombyx mori nuclear polyhedrosis virus (BmNPV) has the highest incidence rate among silkworm diseases. Because the virus is protected by a polyhedron shell outside the body, it exists for a long time and is difficult to disinfect; in addition, due to the long incubation period of the disease, once it breaks out, it will cause Sericulture farmers suffered hea...

Examples

Embodiment 1

[0019] Example 1: A method for inhibiting the proliferation of BmNPV in the silkworm

[0020] ⑴Materials and medicines:

[0021] The silkworm variety: Suxiu×Chunfeng, obtained the silkworm variety certificate issued by the Silkworm Variety Approval Committee of Jiangsu Province (Su Shensi 201001), which has the basic commonality of silkworm.

[0022] Drugs: nano titanium dioxide (TiO 2 ) is a product of Hangzhou Wanjing New Material Co., Ltd.

[0023] Other molecular biology reagents are products of Sangon Bioengineering (Shanghai) Co., Ltd.

[0024] ⑵The use of nano-titanium dioxide

[0025] Mix nano-titanium dioxide with water to prepare an additive solution with a concentration of 5.0 mg / L; fully mix the additive before use, and spray 1.0 L dose per 100 kg of mulberry leaves on mature leaves of various ages. The mulberry variety is Yu 711, After the ripe leaves were dried, silkworms were fed continuously for 3 days, and the amount of mulberry was the same as the usual a...