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Method for extracting antioxidant ingredient from nigella plant seeds based on cloud point extraction and application of antioxidant ingredient

A cloud point extraction method and a technology for antioxidant components, which are applied in the field of chemistry to achieve the effects of simple process, good retention of antioxidant activity and mild extraction conditions

Active Publication Date: 2014-08-06
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, CPE has been widely used in the enrichment and analysis of metal ions, polycyclic aromatic hydrocarbons, and macromolecular substances, and there are relatively few studies on the extraction of natural plant active ingredients.

Method used

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  • Method for extracting antioxidant ingredient from nigella plant seeds based on cloud point extraction and application of antioxidant ingredient
  • Method for extracting antioxidant ingredient from nigella plant seeds based on cloud point extraction and application of antioxidant ingredient
  • Method for extracting antioxidant ingredient from nigella plant seeds based on cloud point extraction and application of antioxidant ingredient

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Experimental program
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Effect test

Embodiment 1

[0023] In this embodiment, the method for extracting antioxidant components in the seeds of Nigella genus based on the cloud point extraction method includes an extraction step, a cloud point phase-separation extraction step and a water removal step, specifically:

[0024] Step 1: Extraction step

[0025] According to the solid-to-liquid ratio g / mL of 1:20, weigh the defatted Nigella genus seed pulverization, add the surfactant polyoxyethylene glyceryl ether monostearate solution with a concentration of 10g / L, and extract 30 Minutes, filtered to obtain solid filter residue and antioxidant active ingredient extract.

[0026] Step 2: Cloud point phase separation extraction step

[0027] Add sodium chloride to the extract solution of antioxidant active ingredients obtained in the extraction step, adjust the ionic strength to 0.5-1.5mol / L, then place the extract solution in a constant temperature water bath, keep the constant temperature balance for 40 minutes, and let it stand o...

Embodiment 2

[0033] In this example, the method for extracting the antioxidant components in the seeds of Nigella genus based on the cloud point extraction method includes an extraction step, a cloud point phase-separation extraction step and an elution step, specifically:

[0034] Step 1: Extraction step

[0035] According to the solid-to-liquid ratio g / mL of 1:35, weigh the crushed defatted Nigella genus seeds, add a surfactant Triton X-114 solution with a concentration of 40g / L, ultrasonically assisted extraction for 40 minutes, and filter to obtain a solid Filter residue and antioxidant active ingredient extract.

[0036] Step 2: Cloud point phase separation extraction step

[0037] Add sodium chloride to the extract solution of antioxidant active ingredients obtained in the extraction step, adjust the ionic strength to 0.5-1.5mol / L, then place the extract solution in a constant temperature water bath, keep the constant temperature balance for 60 minutes, and let it stand or centrifug...

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Abstract

The invention discloses a method for extracting an antioxidant ingredient from nigella plant seeds based on a cloud point extraction and application of the antioxidant ingredient. According to the method, degreased nigella plant seed powder is used as a raw material, and the method comprises the following steps: adding the seed powder into a surfactant solution, sufficiently and uniformly mixing, extracting by assistance of ultrasonic wave to obtain extract and extracted residues; adding an electrolyte into the extract to regulate the ion strength, heating in water bath to a certain temperature and balancing at a constant temperature; standing or centrifuging split phases into an aqueous solution layer and a surfactant enriched layer of the surfactant. The surfactant enriched layer can be treated in two ways respectively: one way is to directly dehydrate to obtain a surfactant mixture with anti-oxidation activity, which can be directly used in appropriate types of cosmetics; the other way is to separate and reclaim the surfactant by adopting a macroporous adsorption resin method, and an antioxidant active crude extract is obtained at the same time. The method is simple in process, does not need toxic or harmful organic solvents, is mild in extraction condition and good in maintenance of antioxidant activity of products, and is advantageous to sufficient utilization of nigella plant seed resource and development of natural antioxidant active cosmetics.

Description

technical field [0001] The invention relates to an extraction and separation method of natural active ingredients and its application, in particular to an extraction method and application of antioxidant active ingredients used in seeds of Ranunculaceae Nigella genus plants, and belongs to the field of chemical technology. Background technique [0002] Nigella nigella is the dry mature seed of Nigella glandulifera Freyn, a plant of Ranunculaceae. It is mainly distributed in Xinjiang and Yunnan of my country. It has been used for thousands of years as a traditional medicinal material of the Uyghur and Dai people, and has been included in the Pharmacopoeia of the People's Republic of China. "In one part, it has the effects of nourishing the kidney and brain, stimulating the menstrual flow, and diuresis. Modern pharmacological studies have shown that black cumin has significant effects on liver and kidney protection and anti-oxidation. At present, most of the pharmacological re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/97A61K36/71A61Q19/08A61P39/06A61K131/00
Inventor 刘学韩王康曹光群杨成曹莹粲
Owner JIANGNAN UNIV
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