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A kind of compound microorganism preparation and its preparation method and application

A technology of compound microorganisms and preparations, applied in the field of micro-ecological preparations and feed additives, to improve the production performance of livestock and poultry, stimulate the immune response of the body, and strengthen the animal immune system

Active Publication Date: 2016-07-27
WUHAN KEQIAN BIOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many feed microecological preparations on the market, the effects of which are mainly focused on providing animal immunity and increasing animal production capacity, and can be directly used for the removal of toxins in animals and the functional use of preventing and treating diarrheal diseases. but rarely reported

Method used

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  • A kind of compound microorganism preparation and its preparation method and application
  • A kind of compound microorganism preparation and its preparation method and application
  • A kind of compound microorganism preparation and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Isolation and identification of Enterococcus faecium SC13:

[0027] The applicant obtained the separation, screening and identification of fresh manure collected from healthy pigs from a natural breeding pig farm in Huangpi, Hubei, and it was deposited at the China Type Culture Collection on March 3, 2014, with the preservation number of CCTCCNO: M2014060.

[0028] The strain is Gram staining positive, oval cells, mainly in pairs, occasionally into short chains, can grow at 10°C and 45°C, ferment glucose to produce acid with a final pH of 4.0-4.4, and can grow in 6.5% NaCl and In pH 9.6 environment, peroxidase is negative, it can decompose sucrose, denbiose, lactose, maltose, raffinose, arabinose and sorbitol, and decompose arginine to produce ammonia. The bile-esculin hydrolysis test is not positive. It does not decompose rhamnose and melezitose, and requires folic acid for growth on a basic synthetic medium. Refer to the Berger's Handbook of Bacteria (eighth edition). This...

Embodiment 2

[0050] A composite microbial preparation for livestock and poultry, the preparation steps are as follows:

[0051] Preparation of Bacillus amyloliquefaciens bacteria powder: Bacillus amyloliquefaciens (CCTCCNO: M2013249) was inoculated into self-prepared fermentation medium, aerated and stirred at 30°C for 44 hours, real-time defoaming and controlled dissolved oxygen above 30% to the rate of spore formation Reach 75%. After the tank is placed, the ceramic membrane is used for microfiltration, and it is concentrated to 1 / 5 of the original volume at a pressure of 0.2MPa, and then enters the drying tower. It is spray-dried in the spray drying tower at 165℃~175℃ with air and 60℃~70℃ with air. Obtain Bacillus amyloliquefaciens bacterial powder, the number of viable bacteria is 2×10 10 CFU / g, used in the following examples;

[0052] Fermentation medium components / L: corn flour 20g / L, soybean meal powder 10-50g / L, yeast extract 15g / L, peptone 10g / L, CaCO 3 2.5g / L, NaCl5g / L, MgSO 4 3g / L, ...

Embodiment 3

[0060] The application of a compound microbial preparation for livestock and poultry in the preparation of a medicine for treating diarrhea, and the application process is as follows:

[0061] From a diarrhea-affected pig farm in Huangpi, 240 weaned piglets of 26-day-old Dabai×Landrace hybrids with an average weight of 7.44±0.25kg were selected and randomly divided into 3 groups, 80 pigs in each group. ,Fed basal diet (blank control group), basal diet + 0.05% (w / w) of the feed additive prepared in Example 2 (experimental group 1), basal diet + 0.2% (w / w) The test period of the feed additive prepared in Example 2 was 28 days. At the beginning and end of the experiment, the individual fasting weights of piglets were weighed to calculate the average daily gain (ADG), feed amount, feed weight gain ratio (F / G), diarrhea rate and mortality rate.

[0062] It can be seen from Table 6 that during the test period, the test group 1 gained an average of 0.98kg more weight than the control gro...

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Abstract

The invention discloses a composite microbial preparation and a preparation method and application thereof. The microbial preparation contains Enterococcus faecium SC13 with an accession number of CCTCC NO: M2014060 and Kluyveromyces marxianus C2 with an accession number of CCTCC NO: M2014059 which are separated by the inventor. Enterococcus faecium SC13 and Kluyveromyces marxianus C2 are combined with Bacillus amyloliquefaciens, jerusalem artichoke powder and food-grade diatomite; through cooperation among bacteria and between the bacteria and a filler, the immune system of an animal is strengthened, proliferation of beneficial bacteria in the intestines of the animal is selectively promoted, colonization and propagation of harmful bacteria are inhibited, the microbial environment in the body of the animal is improved, immune response of organisms is stimulated, immunity and production performance of livestock and poultry are enhanced, usage of antibiotics is reduced, good recognition adhesion and elimination capability on mycotoxins in a feed is obtained, and the conversion rate of the feed is increased.

Description

Technical field [0001] The invention relates to a microbial composite preparation, in particular to a microbial composite preparation for preventing diarrhea and detoxification of livestock and poultry and a preparation method thereof, and belongs to the technical field of microecological preparations and feed additives. Background technique [0002] The accumulation of toxins in the body and diarrhea are the two important causes of high mortality of livestock and poultry and low return rate of breeding. Approximately 25% of grains in the world are contaminated by various molds every year. Due to the different levels of pollution, there is a big gap between different regions. China is the hardest hit area for mycotoxins. According to the investigation of the China Animal Protection Association, during the breeding process, a series of problems such as slow growth of fattening pigs, false estrus of sows, diarrhea of ​​suckling piglets, decreased egg production rate of laying hens,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16A23K10/18C12R1/07C12R1/46C12R1/645
Inventor 徐高原王杨波周明光金建云陈章表李芳韩进蔡皓金梅林陈焕春
Owner WUHAN KEQIAN BIOLOGY CO LTD
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