An ssr marker linked to the lrpw-related qtl of rice stem tip nematode resistance on chromosome 5 and its application

The invention relates to a technology for rice sclerotiorum and rice, which can be applied to the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., and can solve the problems of limiting the effective utilization of rice sclerotia resistance resources, so as to improve the breeding efficiency, The effect of speeding up the breeding process and simplifying the selection method
CN103981180BInactive Publication Date: 2016-09-07JIANGSU ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGSU ACAD OF AGRI SCI
Publication Date
2016-09-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses molecular markers linked with Aphelenchoides besseyi Christie resistant QTL (quantitative trait loci). The essence of the molecular markers is that according to the linkage and segregation rules, the population of the hybrid generation F2 of the Aphelenchoides besseyi Christie resistant variety Tetep and the susceptible variety Huaidao 5 is taken as the mapping population, and the SSR (simple sequence repeat) markers RM163, RM18620, RM440 and RM161, linked with two LRGW (loss rate of panicle weight) related Aphelenchoides besseyi Christie resistant QTL, on the chromosome 5 are obtained through analysis of linkage between the identification results of Aphelenchoides besseyi Christie resistance and the SSR marker data. Whether a plant has Aphelenchoides besseyi Christie resistance can be judged by detecting the SSR marker banding pattern of the single rice plant of the hybrid combination generation of Tetep and derived varieties (lines) thereof and the Aphelenchoides besseyi Christie susceptible variety. After being applied to assistant selection breeding of the Tetep and derived varieties (lines) thereof / susceptible variety-Aphelenchoides besseyi Christie resistant variety, the SSR markers can overcome the defects of poor stability and repeatability of Aphelenchoides besseyi Christie resistance identification, time and labor waste and the like in conventional breeding and can simplify the selection method and improve the breeding efficiency so as to accelerate the breeding process of the Aphelenchoides besseyi Christie resistant variety.
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Description

Technical field:

[0001] The invention relates to molecular markers RM163, RM18620, RM440 and RM161 linked to two LRPW-related QTLs (qLRPW5a, qLRPWSb) for resistance to rice stem nematode resistance on rice chromosome 5, which can be applied to molecular marker-assisted breeding and belong to rice disease-resistant breeding and molecular biology. Background technique:

[0002] Rice dry point nematode is a kind of rice ectoparasitic pathogen, and its typical symptom is that leaf tip twists into dry point, once this disease takes place, it will cause serious loss of grain yield (Journal of the faculty of Agriculture, Kyushu, University, 1950, 9 (3): 309-333; Nematologica, 1975, 21(3): 351-357; Journal of Agricultural Technology, 2011, 337-344). The traditional control methods of A. rice nematode are costly and cause serious environmental problems (Journal of Agricultural Science and Technology, 2011, 14(1): 195-203). Utilizing the resistance of rice varieties is the most econ...

Claims

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