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Preparation method of biochip based on composite nanomaterial and application of such biochip

A technology of biochips and nanomaterials, applied in the direction of nanomedicine, nanotechnology for sensing, nanotechnology, etc., can solve the problems of low protein or DNA attachment rate of biochips, achieve ingenious structural design, small size, and convenient carrying effect

Inactive Publication Date: 2015-08-26
CHANGCHUN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Most of the current biochips use silicon or glass as the substrate to coat a layer of gold nano-film and then directly assemble the protein or DNA on the whole. Although this method can save the production time of biochips, but due to the In the process, no functional group modification was carried out on the gold nanofilm, which would lead to a low attachment rate of the produced biochip protein or DNA

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  • Preparation method of biochip based on composite nanomaterial and application of such biochip
  • Preparation method of biochip based on composite nanomaterial and application of such biochip
  • Preparation method of biochip based on composite nanomaterial and application of such biochip

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Embodiment 1

[0028] (1) First prepare the composite nanomaterials required for the production of biochips. This method uses the oil phase synthesis method to prepare Au-Cu 2 S is used as the inner core, the inner core diameter is 10nm, the outer shell is ZnS, and the shell thickness is 2nm, then the mercaptopolyethylene glycol is dissolved in 4ml of chloroform and stirred for 4 minutes, and the composite nanoparticles made with chloroform and The mixed solution of mercaptopolyethylene glycol was mixed and stirred for 5 minutes at a molar ratio of 1:1, and finally the solution was put into a rotary evaporator to remove chloroform to obtain a composite nanomaterial in the water phase. At this time, the surface of the nanomaterial has been modified. On mercapto.

[0029] (2) Prepare an activation solution with concentrated sulfuric acid and hydrogen peroxide at a volume ratio of 3:1, and pass the activation solution through the surface of a 50nm-thick gold nano film at a constant speed of 100...

Embodiment 2

[0034] (1) First prepare the composite nanomaterials required for the production of biochips. This method uses the oil phase synthesis method to prepare Au-Cu 2 S is used as the inner core, the inner core diameter is 10.6nm, the outer shell is ZnS, and the shell thickness is 1.6nm, then the mercaptopolyethylene glycol is dissolved in 5ml of chloroform and stirred for 5 minutes, and the composite nanoparticle made with The mixed solution of chloroform and mercaptopolyethylene glycol was mixed and stirred for 3 minutes in a molar ratio of 1:1, and finally the solution was put into a rotary evaporator to remove chloroform to obtain a composite nanomaterial of the water phase. At this time, the surface of the nanomaterial Modified sulfhydryl group;

[0035](2) Use concentrated sulfuric acid and hydrogen peroxide to prepare an activation solution with a volume ratio of 3:1, and pass the activation solution through the surface of a 40nm-thick gold nano film at a constant speed of 50...

Embodiment 3

[0040] (1) First prepare the composite nanomaterials required for the production of biochips. This method uses the oil phase synthesis method to prepare Au-Cu 2 S is used as the inner core, the inner core diameter is 9.4nm, the outer shell is ZnS, and the shell thickness is 1.6nm, then the mercaptopolyethylene glycol is dissolved in 3ml of chloroform and stirred for 3 minutes, and the prepared composite nanoparticles are mixed with The mixed solution of chloroform and mercapto polyethylene glycol was mixed and stirred for 8 minutes in a molar ratio of 1:1, and finally the solution was put into a rotary evaporator to remove chloroform to obtain a composite nanomaterial of the water phase. Modified sulfhydryl group;

[0041] (2) Prepare an activation solution with concentrated sulfuric acid and hydrogen peroxide at a ratio of 3:1 by volume, and pass the activation solution through the surface of a 50nm-thick gold nano film at a constant speed of 150ul / s for 15 minutes, using the...

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Abstract

The invention relates to a preparation method of a biochip based on a compound nano material and application of the biochip and relates to preparation and application of biochips. The preparation method of the biochip based on the compound nano material comprises the following steps: carrying out oil phase-water phase transformation on a compound type nano material; treating a gold membrane with an activating solution to remove organic matters on the surface of the gold membrane by utilizing strong oxidizing property of the activating solution; introducing the compound type nano material modified with a sulfydryl into the surface of the gold membrane for assembling; sequentially dipping the obtained gold membrane in a phosphate buffer solution, ultrapure water and mercaptoethanol for fixing the gold membrane; intruding DNA or protein with amino as a biological probe into the obtained gold membrane, so that the biochip is obtained. The preparation method provided by the invention has the advantages that multiple proteins and DNAs can be easily assembled on a substrate to serve as the biochip, and highly sensitive biological detection can be carried out.

Description

technical field [0001] The invention belongs to the technical field of nanomaterial manufacturing, and relates to the preparation and application of biochips. Background technique [0002] Biochip (biochip or bioarray) integrates the biochemical analysis process on the surface of the chip based on the principle of specific interaction between biomolecules, so as to realize high-throughput and rapid detection of DNA, RNA, peptides, proteins and other biological components. The narrow concept of biochip refers to the immobilization of biomolecules (oligonucleotides, cDNA, genomic DNA, polypeptides, antibodies, antigens, etc.) Biomolecular lattices formed on solid phase transmitters such as nylon membranes. Therefore, biochip technology is also called microarray technology, and the solid-phase substrate containing a large amount of biological information is called microarray, also known as biochip. [0003] Nanoparticles refer to particles with a size between 1 and 100 nm. It...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68G01N33/68B82Y5/00B82Y40/00B82Y15/00
CPCC12Q1/6876G01N33/6803
Inventor 刘丽炜王玥胡思怡张佳奇黄晨
Owner CHANGCHUN UNIV OF SCI & TECH