An endophytic fungus that can promote the growth of Chinese fir

Abstract

The present invention provides an endophytic fungus that can promote the growth of Chinese fir, including its isolation, purification, inoculation, cultivation and application methods. The fungus is Cylindrocarpon ? sp. ) CG2 strain, which was registered and preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on May 20, 2014, with the preservation number CGMCC? NO.9188. The bacterial strain involved in the present invention is obtained by separating and purifying from Chinese fir plants. After being inoculated in Chinese fir tissue culture seedlings, it is further confirmed that it has a promoting effect on plant growth according to various indicators of photosynthesis and biological characteristics. Beneficial functional endophytic fungi that promote plant growth can be applied to production.

Description

technical field [0001] The invention relates to an endophytic fungus ( Cylindrocarponsp. ), including its isolation, purification, inoculation and culture, and its application methods. Background technique [0002] The study of plant endophytes began at the end of the 19th century when Vogl isolated the first endophytes from the seeds of Lolium temulentum L. ryegrass. However, a large number of studies on endophytes in plants began in the 1980s, mainly in vegetation in temperate, subtropical and tropical regions. [0003] The predecessors can isolate endophytes from most plants, so it can be speculated that endophytes are ubiquitous in plants. Endophytes have been found in at least 80 genera and 290 species of grass crops worldwide. At present, the endophytes isolated from plants can be divided into three types according to their unique functions: plant growth-promoting bacteria such as nitrogen-fixing bacteria, potassium-fixing bacteria, and phosphorus-fixing bacteria; ...

AI Technical Summary

Problems solved by technology

In summary, the research on the endophytic fungi of Chinese fir is only carried out on the identification of the root, but the research on the endophytic fungi is not involved in the leaves and stems of Chinese fir.

In addition, there is still a gap in the research on the influence of endophytic fungi on the growth of Chinese fir itself.

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