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A low-temperature and high-efficiency corn stalk degrading composite bacterial system and its application

A technology of corn stalks and composite bacteria, applied in the field of microorganisms, can solve the problems of low enzyme activity, low cellulase activity, and easy degradation, and achieve the effects of improving soil fertility, increasing various enzyme activities, and efficiently degrading

Inactive Publication Date: 2016-06-22
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Cellulase in soil plays a very important role in promoting the degradation of straw, but the activity of cellulase in soil is low under natural conditions. By using straw degrading bacteria, the speed of in situ decomposition of straw organic matter is accelerated, which is to promote the in situ degradation of straw. new way of
In order to improve the degradation effect of straw, people have done a lot of research on the application of microbial inoculum in straw returning to the field, and some commercially available microbial inoculants have appeared, but the adaptation temperature of these microbial inoculants is all medium and high temperature (15-50°C), while The climate in the northern region is relatively cold, and medium-high temperature microbial agents cannot adapt, and are prone to problems such as low enzyme activity, poor stability, easy degradation, and poor cold resistance, which cannot benefit the effect of straw returning to the field. Efficient straw-degrading bacteria are of great significance for straw returning to the field

Method used

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  • A low-temperature and high-efficiency corn stalk degrading composite bacterial system and its application
  • A low-temperature and high-efficiency corn stalk degrading composite bacterial system and its application
  • A low-temperature and high-efficiency corn stalk degrading composite bacterial system and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: the screening acclimation of compound strain of the present invention

[0045] Enrichment culture:

[0046] Wash and dry the natural corn stalks harvested in autumn to constant weight, cut them into lengths of about 1-2 cm, and sterilize them at 121°C and 101Pa for 30 minutes for later use.

[0047] The soil extract is prepared by mixing soil and deionized water at a mass ratio of 1:1, bathing in water at 60°C for 2 hours, and filtering and sterilizing

[0048] The soil of Changchun City's corn stalks returned to the field for many years containing Cellvibrio, Azospira, Arcobacter, Azonexus, Clostridium, Paludibacter, Lotharella, and Trichosporon bacteria was inoculated into a WhatmanNO1 filter paper strip (2*6cm , half submerged below the liquid level of the culture medium, and half above the liquid level) in the enrichment medium, gently rotated at 50rpm / min for 30min and then cultured statically at 25°C. The enrichment medium contains 5g / L peptone, 1g / ...

Embodiment 2

[0057] Embodiment 2: the identification of composite bacterial strain of the present invention

[0058] The composite bacterial strain screened in Example 1 was used to determine the strain composition of the strain 16S-rDNA and 18S-rDNA sequences, and the results are shown in Table 1.

[0059] Table 1 Composition of microbial strains of complex bacterial strains

[0060]

[0061] According to the method of Example 1, cellulose-rich corn stalk returning soil, cattle and sheep dung, and grassland meadow soil were respectively used as bacterial source samples in the alpine regions of northern my country. A total of 128 samples were used to screen the composite bacterial strains. 198 copies of the composite strains were screened out, and the composition of the strains was determined by the 16S-rDNA and 18S-rDNA sequences of the strains. The results showed that each strain in the screened composite strains belonged to Cellvibrio, Azospira, Arcobacter , Azonexus, Clostridium, Pal...

Embodiment 3

[0062] Embodiment 3: Degradation rate determination of corn stalks

[0063] Put 10g / Kg of straw / soil in a 2L sterilized glass beaker, cultivate it in the dark at 10°C for 60 days, and measure the soil water content regularly to maintain soil humidity. Sampling was carried out every 15 days from the beginning of the test until the end of the 60th day. The three-point vertical method was used to collect, and it was fully mixed for later use, with 5 repetitions.

[0064] Set treatment: GF-20: soil + straw + compound bacterial strain of Example 1 of the present invention

[0065] CK: soil + straw

[0066] Straw degradation rate = (weight of corn stalks before cultivation - weight of corn stalks after cultivation) / weight of corn stalks before cultivation

[0067] See the test resultsfigure 1 . Depend on figure 1 It can be seen that the straw degradation rate gradually increases with the prolongation of the soil cultivation time for the two treatments; among different treatmen...

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Abstract

The invention relates to the field of microorganisms, and specifically discloses a low-temperature and high-efficiency corn stalk-degrading composite bacterial system and its application. The composite bacterial strain of the present invention includes bacteria and fungi, the bacteria include Cellvibrio, Azospira, Arcobacter, Azonexus, Clostridium, and Paludibacter bacteria, and the fungi include Lotharella and Trichosporon bacteria. In the present invention, the bacterial genera with a specific composition are taken as the bacterial source samples, and the culture medium composed of specific bacterial genera is screened and domesticated through enrichment culture, subculture, enzyme production culture and corn stalk degradation culture by using a suitable culture medium. The composite bacteria system can efficiently degrade corn stalks under low temperature conditions, making up for the defect that existing microbial agents can only degrade straws under medium and high temperature conditions.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a low-temperature and high-efficiency corn stalk-degrading composite bacterial line and its application. Background technique [0002] Straw cellulose is the most abundant and widely sourced organic matter on earth. Returning corn stalks in situ can not only improve the comprehensive utilization rate of corn stalks, but also is an effective technical measure to fertilize and improve soil and improve soil fertility. However, due to the cold and dry climate in northern my country, corn stalks are not easy to decompose in a short period of time, and the direct return to the field will affect the moisture and cultivation. Abandonment and incineration not only cause waste of resources, but also cause environmental pollution. Since the cellulose macromolecules in the straw with a content of 40% to 50% are surrounded by lignin, and the lignin molecules are very stable and not easy to degr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14C05F11/08C12R1/01C12R1/145C12R1/645
Inventor 高聚林青格尔于晓芳胡树平闹干朝鲁孙继颖王振王志刚苏志军谢岷萨如拉高琳胡海红
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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