A kind of beer and preparation method thereof
A technology for beer and fresh lotus leaves, which is applied in the field of beer and its preparation, can solve the problems of injuring the spleen and stomach, aggravating the moisture in the body, etc., and achieves the effects of good efficacy, easy operation and simple processing method.
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Embodiment 1
[0022] A kind of preparation method of beer is as follows:
[0023] (A) crushing the fresh lotus leaves in the air, sequentially adopting cellulase pretreatment, dilute hydrochloric acid leaching, ultrasonic-assisted extraction, and chloroform extraction to extract the nuciferine in the fresh lotus leaves;
[0024] (B) 0.6 g of nuciferine and 100 kg of beer after the fermentation step are mixed and stirred evenly, and then sterilized, clarified with a clarifying agent, frozen, filtered and packaged to obtain a finished product.
Embodiment 2
[0026] (A) The fresh lotus leaves are air-dried until the mass fraction of the water content is 18%, then pulverized, followed by cellulase pretreatment, dilute hydrochloric acid leaching, ultrasonic-assisted extraction, and chloroform extraction to extract the nuciferine in the fresh lotus leaves, The mass fraction of its purity by detecting nuciferine is 98%; wherein the cellulase pretreatment adopts an amount of 2.667×10 -6 mo1 / (s.g) of cellulase was pretreated at a temperature of 50°C, the enzymolysis time was 2 hours, the pH value of the enzymolysis was controlled at 5.0, and dilute hydrochloric acid leaching was carried out with dilute hydrochloric acid with a mass percentage concentration of 0.5%. Hydrochloric acid leaching for 16h;
[0027] (B) 0.9 g of nuciferine and 100 kg of beer after normal fermentation steps are mixed and stirred evenly, and the finished product is obtained after sterilization, clarification with a clarifying agent, freezing, filtration and packa...
Embodiment 3
[0029] (A) The fresh lotus leaves are air-dried until the mass fraction of the water content is 17%, then pulverized, followed by cellulase pretreatment, dilute hydrochloric acid leaching, ultrasonic-assisted extraction, and chloroform extraction to extract the nuciferine in the fresh lotus leaves, The mass fraction of its purity by detecting nuciferine is 99%; wherein the cellulase pretreatment adopts an amount of 3.000×10 -6 mo1 / (s.g) of cellulase was pretreated at a temperature of 60°C, the enzymolysis time was 2 hours, the pH value of the enzymolysis was controlled at 6.0, and the dilute hydrochloric acid leaching was performed with dilute hydrochloric acid with a mass percentage concentration of 0.8%. Hydrochloric acid leaching for 18 hours; the specific steps of ultrasonic-assisted extraction are to put the extraction solvent into fresh lotus leaves leached with dilute hydrochloric acid, ultrasonically vibrate for 30 minutes, control the temperature at 78°C, and control t...
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