Ternary nano composite drug, preparation method thereof, and application of the drug in preparation of pharmaceutical composition for treating tumors

A nano-composite and drug technology is used in the preparation of a pharmaceutical composition for treating tumors, its preparation, the treatment of multidrug-resistant tumors, the preparation of a pharmaceutical composition for treating tumors by using ultrasonic excitation, and the field of ternary nano-composite drugs. Solve the problems of chemotherapy drug side effects, limited excitation source, multidrug resistance of tumor cells, etc.

Active Publication Date: 2014-10-01
NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problem that the excitation source of semiconductor material photodynamic therapy is limited, and to solve the side effects of chemotherapy drugs and the phenomenon of multidrug resistance of tumor cells in the process of tumor treatment

Method used

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  • Ternary nano composite drug, preparation method thereof, and application of the drug in preparation of pharmaceutical composition for treating tumors
  • Ternary nano composite drug, preparation method thereof, and application of the drug in preparation of pharmaceutical composition for treating tumors
  • Ternary nano composite drug, preparation method thereof, and application of the drug in preparation of pharmaceutical composition for treating tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0113] Example 1: PEG-TiO 2 -Adriamycin ternary nanocomposite drug

[0114] (1.1) Preparation:

[0115] (a) TiO 2 -Adriamycin binary nanocomposite drug:

[0116] (a-1) Preparation of TiO 2 -Adriamycin binary nanocomposite drug:

[0117] Take TiO with a particle size of 30-50nm and ultrasonic activation properties 2 The powder (refer to the papers published by the applicant for the preparation method: A Wu, T Paunesku, EMB Brown, A Babbo, C Cruz, M Aslam, et al.NANO2008, 3, 27-36.) was dissolved in ultrapure water, prepared Get 0.075mol / L TiO 2 Nanoparticle aqueous sol 100mL, shielded from light, stirred under nitrogen gas for 0.5 hours; under stirring, added 8.25mL of 0.005mol / L aqueous solution of doxorubicin dropwise, shielded from light, stirred in airtight air for 12 hours, so that doxorubicin was fully adsorbed to TiO 2 Nanoparticle surface; after stirring, transfer the mixture solution to a centrifuge tube for centrifugation at a speed of 12,000 rpm for 30 minute...

Embodiment 2

[0136] Example 2: PEG-Fe 3 o 4 -TiO 2 -Adriamycin ternary nanocomposite drug

[0137] (2.1) Preparation:

[0138] (a) Preparation of Fe 3 o 4 -TiO 2 -Adriamycin binary nanocomposite drug:

[0139] Take the particle size as 40-60nm nanometers, with T 2 Weighted MR signals with ultrasound-activated properties of Fe 3 o 4 -TiO 2 Nanocomposite powder (its preparation method can refer to the Chinese invention patent CN102125699A that the aforementioned applicant has applied for) is dissolved in ultrapure water to obtain 0.1mol / L Fe 3 o 4 -TiO 2 Nanoparticle water sol 90mL, shielded from light, stirred with nitrogen for 0.5 hours; under stirring, added 10mL of 0.005mol / L doxorubicin solution dropwise, shielded from light, stirred in airtight air for 24 hours, so that doxorubicin was fully adsorbed to Fe 3 o 4 -TiO 2 surface; after stirring, transfer the mixture solution to a centrifuge tube for centrifugation at a speed of 12,000 rpm and a centrifugation time of 30 mi...

Embodiment 3

[0147] Example 3: BSA-Fe 3 o 4 -TiO 2 -Adriamycin ternary nanocomposite drug

[0148] (a) Preparation of Fe 3 o 4 -TiO 2 -Adriamycin binary nanocomposite drug: the same as (2.1).

[0149] (b) Preparation of BSA-Fe 3 o 4 -TiO 2 - Doxorubicin ternary nanocomposite drug:

[0150] The Fe prepared by (a) 3 o 4 -TiO 2 - The doxorubicin binary nanocomposite drug was freeze-dried to obtain a powder, 50 mg of the powder was dissolved in 10 mL of ultrapure water, and prepared into a 5 mg / mL hydrosol. Prepare 50 mL of 0.8 mg / mL sodium chloride solution, and adjust the pH value of the solution to 8-11 with sodium hydroxide. Weigh 50 mg of BSA and dissolve it in sodium chloride solution to obtain a 1 mg / mL BSA solution. At room temperature, under magnetic stirring in the dark, the Fe 3 o 4 -TiO 2 - Doxorubicin was added dropwise to the BSA solution, and 6 mL of absolute ethanol was added dropwise, and then 50 μL of 10% glutaraldehyde solution was added dropwise, and the ag...

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Abstract

The invention relates to a ternary nano composite drug, a preparation method thereof, and an application of the drug in preparation of a pharmaceutical composition for treating tumors. The ternary nano composite drug comprises a core carrier, an active pharmaceutical ingredient, and a stabilizing agent which is used to cover the core carrier and the active pharmaceutical ingredient. The core carrier comprises a first nano particle, which can generate active free radicals by supersonic wave excitation, a composite nano particle having medicinal imaging signals on the basis of the first nano particle, or a combination of the first nano particle and the composite nano particle. The pharmaceutical ingredient is loaded on the core carrier. The stabilizing agent comprises hydrophilic polymer, liposome with hydroxyl groups or carboxylic groups on the surface, bovine serum albumin nano particles, human serum albumin nano particles, or combination thereof. The invention also relates to an application of a binary nano composite drug in preparation of a pharmaceutical composition which can be used to cure tumors through supersonic wave excitation. The tumor target treatment can be realized through the synergetic cooperation between supersonic wave treatment and chemotherapy.

Description

technical field [0001] The invention relates to a nanocomposite drug, in particular to a ternary nanocomposite drug, its preparation method and its use in preparing a pharmaceutical composition for treating tumors. The present invention also relates to the use of the ternary nanocomposite drug and the binary nanocomposite drug in preparing a pharmaceutical composition for treating tumors by ultrasonic excitation. The invention can realize the synergistic treatment of malignant tumors with chemical drugs and ultrasonic waves, especially the treatment of multi-drug resistant tumors. Background technique [0002] Chemotherapy is the main method of clinical treatment of malignant tumors. However, while chemotherapy drugs kill tumor cells, they also damage normal cells to varying degrees, destroy the body's immune system, and affect the quality of life of patients. Severe side effects of chemotherapy can lead to low immunity and physical weakness in cancer patients, which is on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K45/00A61K49/00A61K47/42A61K47/36A61K47/34A61K47/32A61K47/18A61K47/22A61P35/00
Inventor 任文智吴爱国曾乐勇沈折玉马雪华
Owner NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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