Electrode material based on graphene oxide/graphite fiber composite structure and its preparation and application method
A graphite fiber and composite structure technology, applied in the direction of material electrochemical variables, can solve the problems that the sensitivity is difficult to achieve a large breakthrough, graphene oxide is easy to agglomerate, and the basic electrode area is small, which is beneficial to fixation and detection sensitivity and stability Improved performance, simple method, and wide application range
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Embodiment 1
[0028] Embodiment 1: Preparation of electrochemical DNA sensor
[0029] 1) Oxidation of commercial graphite fibers: Mix 100ml of concentrated sulfuric acid, 10ml of concentrated nitric acid, and 4g of potassium permanganate as an oxidation solution. Put the fiber directly into the above oxidation solution, oxidize at 65°C for 12 hours. The fibers were removed and rinsed copiously with deionized water.
[0030] 2) The oxidized fiber was characterized by electron microscopy, infrared spectroscopy and X-ray photoelectron spectroscopy, and it was confirmed that a large number of ordered graphene oxide (abbreviated as GO, the same below) sheets were formed on the surface.
[0031] 3) Cut out a piece of oxidized fiber, stick it on the conductive glass with silver glue, and connect a piece of copper wire in front as the electrode wire. Leave a fiber with a length of 1.5 cm for subsequent testing, and the other parts are sealed by epoxy resin and polytetrafluoroethylene tape. Used ...
Embodiment 2
[0033] Embodiment 2: Preparation of electrochemical enzyme sensor
[0034] 1) Oxidize the commercial graphite fiber: 90ml of concentrated sulfuric acid, 10ml of concentrated phosphoric acid, 4.5g of potassium permanganate and mix well as the oxidation solution. Put the fiber directly into the above oxidation solution, and oxidize for 20 hours at 70°C. The fibers were removed and rinsed copiously with deionized water.
[0035] 2) The oxidized fiber was characterized by electron microscopy, infrared spectroscopy and X-ray photoelectron spectroscopy, and it was confirmed that a large number of ordered GO sheets were formed on the surface.
[0036] 3) Cut out a piece of oxidized fiber, stick it on the conductive glass with silver glue, and connect a piece of copper wire in front as the electrode wire. Leave the fiber with a length of 1 cm for subsequent detection, and the other parts are sealed by epoxy resin and polytetrafluoroethylene tape. Used as the working electrode of th...
Embodiment 3
[0038] Example 3: Preparation of electrochemical immunosensor
[0039] 1) Oxidize the commercial graphite fiber: 90ml of concentrated sulfuric acid, 10ml of concentrated phosphoric acid, 4.5g of potassium permanganate and mix well as the oxidation solution. Put the fiber directly into the above oxidation solution, oxidize for 24 hours at 80°C. The fibers were removed and rinsed copiously with deionized water.
[0040] 2) The oxidized fiber was characterized by electron microscopy, infrared spectroscopy and X-ray photoelectron spectroscopy, and it was confirmed that a large number of ordered GO sheets were formed on the surface.
[0041] 3) Cut out a piece of oxidized fiber, stick it on the conductive glass with silver glue, and connect a piece of copper wire in front as the electrode wire. The fiber with a length of 0.5 cm was left for subsequent detection, and the other parts were sealed by epoxy resin and polytetrafluoroethylene tape. Used as the working electrode of the ...
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