Application of algal polysaccharide
A seaweed polysaccharide, marine technology, applied in the application field of seaweed polysaccharide, to achieve the effect of abundant raw material sources, low production cost and simple preparation process
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Embodiment 1
[0013] Take 20 g of crushed centipede algae (Grateloupia filicina), add 600 g of distilled water, stir in a water bath at 90°C for 4 hours, filter out the filter residue with 100 mesh, 200 mesh and 300 mesh sieves successively, and concentrate the filtrate with a vacuum concentration equipment 1 / 10 of the original volume, then ethanol precipitation with 4 times the volume of the concentrated solution in 95% ethanol for 24 hours, centrifuged, and the precipitate was freeze-dried to obtain the centipede polysaccharide.
[0014] The centipede algae polysaccharide obtained above is formulated into a 1% (mass percentage) aqueous solution for use. Commercially available probiotics (mainly a mixture of probiotics such as lactic acid bacteria and bifidobacteria) were activated and amplified with MRS medium. The amplified bacterial solution was placed in a 96-well plate, and different volumes of centipede polysaccharide solutions were taken to make the final concentrations 0.1% and 0.5...
Embodiment 2
[0016] Take 20 g of crushed Eucheuma (E.maricatum), add 800 g of distilled water, stir in a water bath at 70°C for 2 hours, filter out the filter residue with 100 mesh, 200 mesh and 300 mesh sieves successively, and use vacuum concentration equipment for the filtrate. Concentrate to 1 / 10 of the original volume, then ethanol precipitate with 4 times the volume of the concentrated solution in 95% ethanol for 24 hours, centrifuge, and freeze-dry the precipitate to obtain the Eucheuma polysaccharide.
[0017] The Eucheuma polysaccharide obtained above is formulated into a 1% (mass percentage) aqueous solution for use. Commercially available probiotics (mainly a mixture of probiotics such as lactic acid bacteria and bifidobacteria) were activated and amplified with MRS medium. The amplified bacterial liquid was placed in a 96-well plate, and different volumes of Euglena polysaccharide solutions were taken to make the final concentrations 0.1% and 0.5%, and the control was a mixture...
Embodiment 3
[0019] Take 20 g of crushed Gelidium amansii, add 600 g of distilled water, stir in a water bath at 100°C for 2 hours, filter out the filter residue with 100 mesh, 200 mesh and 300 mesh sieves successively, and concentrate the filtrate with a vacuum concentration equipment 1 / 10 of the original volume, then ethanol precipitation with 4 times the volume of the concentrated solution in 95% ethanol for 24 hours, centrifuged, and the precipitate was freeze-dried to obtain the centipede polysaccharide.
[0020] The agaricus polysaccharide obtained above is prepared into a 1% (mass percentage) aqueous solution for use. Commercially available probiotics (mainly a mixture of probiotics such as lactic acid bacteria and bifidobacteria) were activated and amplified with MRS medium. The amplified bacterial solution was taken and placed in a 96-well plate, and different volumes of agaricus polysaccharide solutions were taken to make the final concentrations 0.1% and 0.5%, and the control wa...
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