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Animal Bifidobacterium and use method thereof

A technology of animal bifidobacteria and Escherichia coli, which is applied in the fields of application, animal feed, animal feed, etc., can solve the problems of large differences in strains, and achieve the effects of extending shelf life, expanding the scope of application, and improving flavor

Active Publication Date: 2012-09-26
BEIJING BOJINYUAN BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the existing bifidobacteria, due to the large differences between the strains, are often unable to survive at room temperature for a long time with the functions of acid resistance and bile salts, and they also have antibacterial properties, and can be compounded with lactic acid bacteria, etc. advantage

Method used

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  • Animal Bifidobacterium and use method thereof
  • Animal Bifidobacterium and use method thereof
  • Animal Bifidobacterium and use method thereof

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Experimental program
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Embodiment

[0032] Embodiment, this animal Bifidobacterium, it is Bifidobacterium animalis (Bifidobactrium animalis) B174, preservation number: CGCC NO.4182, preservation date: September 16, 2010, preservation unit: China Microbial Culture General Microbiology Center of the Preservation Management Committee, address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0033] The isolation and identification methods of Bifidobacterium animalis milk subsp. B174 are as follows:

[0034] 1. Separation method

[0035] Weigh 5g of the sample (naturally fermented food) into 45ml of sterile buffered saline solution (with appropriate amount of glass beads) as the mother solution, oscillate for 10 minutes to mix thoroughly, and make gradient dilution with sterile buffered saline solution, choose three suitable gradients, and draw 0.1ml in NPNL-BL medium for rolling tube separation, do two repetitions for each dilution gradient, put the anaerobic tube in a 37°C incubator for 48-72 h...

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Abstract

The invention relates to an animal Bifidobacterium and a use method thereof. The animal Bifidobacterium is the Bifidobactrium amimalis Subsp Lactis B174, the CGMCC No. is 4182, the conservation date is September 16, 2010 and the conservation unit is the China General Microbiological Culture Collection Center. The Bifidobactrium amimalis Subsp Lactis B174 can colonize in human body and resist the gastric acid and cholate of human body. Meanwhile, the strain has better inhibitory action on Escherichia coli and can survive for 30 days at the room temperature. The strain and other lactic acid bacteria have better synergic action for growth; the strain has better combination effect with lactic acid bacteria; the product has better flavor, can be used to prepare fermented yoghurt, animal feed additives and antibacterial solution and can be widely used in the fields of other foods and biological products.

Description

technical field [0001] The invention relates to an animal bifidobacterium and an application method thereof. Background technique [0002] Bifidobacterium is a typical beneficial bacterium in the human intestinal tract. It is the first physiological bacterium discovered by human beings. Its growth and reproduction exist in human life. It is a group of probiotics that can colonize the intestinal tract of healthy people. Its existence can reduce the PH value of the human body system, thereby changing the intestinal flora, inhibiting and killing pathogenic bacteria, thereby enhancing the immune resistance of the human body. Bifidobacteria can synthesize a variety of vitamins in the intestinal tract. Laxative, lower cholesterol and triglycerides, inhibit cancer cells, anti-aging and other effects. Therefore, it has been scientifically confirmed that the quantity and quality of bifidobacteria in the human body is one of the important criteria for human health. [0003] Diverse ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K1/16A23C9/127A23K10/18
Inventor 刘英华姜波苏桂
Owner BEIJING BOJINYUAN BIOTECH
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