Nasal-spraying immune influenza multivalent vaccine and preparation method thereof

A technology of influenza vaccine and multivalent vaccine, applied in the field of vaccines, can solve the problems of immune protection without mucosal immune effect, achieve the effect of preventing highly pathogenic human avian influenza, avoiding pain and cross-infection, and simple preparation

Inactive Publication Date: 2009-06-10
MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As far as the influenza vaccine is immunized by injection, the immune response and immune protection caused by it have no local mucos

Method used

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  • Nasal-spraying immune influenza multivalent vaccine and preparation method thereof
  • Nasal-spraying immune influenza multivalent vaccine and preparation method thereof
  • Nasal-spraying immune influenza multivalent vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0036] Example 1: Preparation of influenza tetravalent (H1N1, H3N2, H5N1 and B type) live attenuated vaccine strain

[0037] Type A influenza virus strains H1, H3, H5 and type B influenza virus strains were provided by China CDC and Hualan Biotechnology Co., Ltd. Use traditional chicken embryo allantoic cavity culture influenza virus method or microcarrier suspension culture and tamed mammalian cells (such as Vero cells, MDCK cells, 2BS cells, etc.) to cultivate influenza viruses.

[0038] ①Using the existing reverse genetics method, the type A multivalent virus uses the cold-adapted attenuated influenza virus strain A / Ann Arbor / 6 / 60 as the viral backbone, and the type B multivalent virus uses B / Ann Arbor / 1 / 66 as the virus backbone. Rescue the virus backbone and integrate the HA and NA surface antigen genes of the influenza A and B strains that prevailed that year. That is to say, 8, 3 or 4 recombinant plasmids constructed by H1N1, H3N2, H5N1 and B influenza virus gene fragments w...

Example Embodiment

[0042] Example 2: Preparation of influenza multivalent (H1N1, H3N2, B, H5N1, H7N7, H9N2 subtypes) live attenuated vaccine antigen

[0043] Type A influenza virus strains H1N1, H3N2, H5N1, H7N7, H9N2 subtype and type B influenza virus strains were provided by China CDC and Hualan Biotechnology Co., Ltd. Use traditional chicken embryo allantoic cavity culture influenza virus method or microcarrier suspension culture and tamed mammalian cells (such as Vero cells, MDCK cells, 2BS cells, etc.) to cultivate influenza viruses.

[0044] ①Using the existing reverse genetics method, the type A multivalent vaccine antigen uses the cold-adapted attenuated influenza virus strain A / Ann Arbor / 6 / 60 as the virus backbone, and the type B multivalent vaccine antigen uses B / Ann Arbor / 1 / 66 In order to rescue the virus backbone, it integrates the HA and NA surface antigen genes of influenza A and B influenza virus strains that year. That is, 8, 3 or 4 recombinant plasmids constructed by any combinatio...

Example Embodiment

[0048] Example 3: Observation of in vivo effects of influenza tetravalent live attenuated vaccine in mice

[0049] 1. Immunization strategy, using different dosage forms of influenza virus vaccine to immunize mice

[0050] ①According to the method of preparing influenza tetravalent (H1N1, H3N2, H5N1 and B) live attenuated virus strains in Example 1, at the same time, dilute the antigen with a stabilizer to the amount of live attenuated vaccine ≥5×10 6 EID 50 , Which inactivated influenza vaccine with nasal spray + AL(OH) 3 The adjuvant group is the control (Tab1).

[0051] ②Six groups of Balb / c mice received different dosage forms of vaccines for nasal immunization, each mouse with 20μl antigen solution, among which the inactivated influenza vaccine contains AL(OH) 3 Adjuvant. Immunization was performed on the 0th and 28th days, and the IgG, IgA and HAI titers in serum, nasal irrigation fluid and lung lavage fluid were measured on the 42nd day.

[0052] Table 1 Group design of Bal...

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Abstract

The invention discloses a nasal spray immunity flu polyvalent vaccine and a preparing method. Flu quadrivalence (H1N1, H3N2, H5N1, B type) or polyvalent attenuated live vaccine is used for immunoprophylaxis through nose spraying, popular flu and highly pathogenic avian influenza can be prevented safely and efficiently.

Description

technical field [0001] The invention relates to a vaccine, in particular to a multivalent influenza vaccine for nasal spray immunization. Background technique [0002] Influenza is an acute febrile respiratory infectious disease caused by influenza virus, which can cause serious complications. The antigens of influenza virus are constantly changing, and they are highly contagious, often causing large-scale epidemics. In a normal epidemic season, about 10% of the world's population, or more than 500 million people, suffer from influenza. At present, no ideal prevention and treatment drugs have been found. Influenza vaccination is still an effective means of preventing influenza today. Influenza vaccination of healthy people will prevent the disease in 70-90% of the people (when the circulating virus strain is the same as the vaccine strain), and more importantly, vaccination of high-risk groups can significantly reduce the incidence of influenza. occurrence, reducing hospit...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61K39/145A61K47/42A61K9/72A61K9/12A61K9/14A61K9/16A61K45/06A61P31/16
Inventor 杨鹏辉王希良罗德炎段跃强邢丽王金成
Owner MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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