Preparation method for peanut meal and natto sauce
A technology of natto paste and peanut meal, which is applied in the field of preparation of peanut paste natto paste, can solve the problems of natto bitterness and odor, affect the taste of natto, inconvenient eating, etc., and achieve reduction The production of unpleasant flavor substances, the effect of good nutrition and health care, and the effect of convenient preparation
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[0011] The present invention will be further described below in combination with specific embodiments.
[0012] Activation of Bacillus natto and Preparation of Seed Solution
[0013] Under aseptic conditions, put Japanese-made natto into a sterile test tube, add 4.5ml sterile water and shake well, after dilution by 10-fold gradient dilution method, in the seed medium (beef extract 5g / L, peptone 10g / L L, yeast extract 5g / L, agar 10g / L, NaCl 5g / L, adjust the pH to 7.0-7.2, 0.1-0.2MPa, 20min) draw a line on the solid surface, incubate upside down at 37°C for 24h. After 24 hours, the medium was taken out to observe the growth of the strains, and a typical colony was picked with an inoculation needle for secondary plate culture.
[0014] The activated Bacillus natto is picked with an inoculation loop and inoculated in a 200ml liquid medium (beef extract 5g / L, peptone 10g / L, yeast extract 5g / L, glucose 10g / L, NaCl 3g / L, pH7 .0~7.2, 0.1~0.2MPa, 20min, standby), 37°C, 180r / min, shak...
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