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A method of controlling the total number of bacteria carried by copepods

A technology for counting copepods and bacteria, which is applied in the field of Bdellovibrio, can solve problems such as unsatisfactory needs, and achieve the effects of simple and easy preparation methods, slow regeneration, and wide selection of materials

Active Publication Date: 2016-08-24
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to effectively ensure the safety of aquaculture water and live biological bait copepods, simple disinfection methods can no longer meet the needs

Method used

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  • A method of controlling the total number of bacteria carried by copepods
  • A method of controlling the total number of bacteria carried by copepods
  • A method of controlling the total number of bacteria carried by copepods

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Experimental program
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Embodiment 1

[0044] The specific experimental method is as follows:

[0045] 1. Materials, media and solutions

[0046] (1) Material

[0047] Bdellovibrio: BDS02.

[0048] Host bacteria: Escherichia coli GIM1.355, purchased from the Culture Collection Center of Guangdong Institute of Microbiology.

[0049] Water samples containing copepoda (Copepoda): collected from a mariculture farm in Puqian Town, Wenchang City, Hainan Province. The number of samples was about 3 / mL.

[0050] (2) culture medium

[0051] 2216E Zobel Marine Bacteria Medium: 15g sea crystal, 5g peptone, 1g yeast extract, 0.01g ferric phosphate, 1000mL distilled water, pH7.6~7.8, 15g agar. After sterilizing at 121°C and 0.1MPa for 20 minutes, pour it into a plate and save it for later use.

[0052] TCBS agar medium (Vibrio selective medium): 89g of TCBS agar, add 1000mL of distilled water, stir, heat and boil until completely dissolved, pour into a sterilized plate and save for later use.

[0053] Nutrient broth liqui...

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Abstract

The invention discloses a method for controlling the total count of bacteria carried by copepoda. The method comprises the following steps: preparing a Bdellovibrio sp. diluent of which the concentration is 2*10<2>-2*10<5>PFU / mL, and mixing the Bdellovibrio sp. diluent with a mixed solution of sodium glutamate and cane sugar in the volume ratio 1:1 to obtain a Bdellovibrio sp. preparation; and mixing the Bdellovibrio sp. preparation with a pretreated copepoda-containing water sample. The method provided by the invention is simple and feasible, and is suitable for industrial batch production. The contained sodium glutamate and cane sugar do not have toxic or side effects on water bodies and the copepoda, and do not cause pollution to the water bodies or death of beneficial aquatic plankton such as the copepoda. Through the method disclosed by the invention, the total count of the bacteria carried by the copepoda can be effectively controlled within 103cfu / mL in 24 hours, and the regeneration of the bacteria is slowed down for a long period of time.

Description

technical field [0001] The invention belongs to the technical field of Bdellovibrio, in particular to a method for controlling the total number of bacteria carried by copepods. Background technique [0002] Copepoda, belonging to the phylum Arthropoda, Crustacea, subclass Copepoda. It is a small crustacean with a body length of <3mm. It lives as a planktonic and parasitic animal and is distributed in ocean, freshwater or brackish water. Copepods move rapidly and have a relatively long generation cycle. Copepods and Cladocera are also used as bait in aquaculture. Copepods are very important as aquatic beneficial plankton. It is not only an important bait for various economic fish, such as bighead carp, herring, mackerel, various juvenile fish and baleen whales, but also can be used as a symbol of fishing grounds. Some fish specialize in preying on copepods, so the distribution of copepods and The migration routes of fish schools are closely related. The output of some ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00C02F3/34A61K35/74C12N1/20C12R1/01A61P31/04
CPCY02A40/81Y02A50/30
Inventor 蔡俊鹏孙秋平郭衍彪
Owner SOUTH CHINA UNIV OF TECH
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