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Method for controlling total count of bacteria carried by artemia

A technology of bacteria count and Artemia, applied in the field of controlling the number of longitudinal bacteria carried by Artemia, achieving the effects of low price, simple and easy preparation method, and wide selection of materials

Inactive Publication Date: 2014-11-05
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is no report on the application of Bdellovibrio preparations to control the total bacteria carried by high-concentration Artemia in China.

Method used

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  • Method for controlling total count of bacteria carried by artemia
  • Method for controlling total count of bacteria carried by artemia
  • Method for controlling total count of bacteria carried by artemia

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Experimental program
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Embodiment 1

[0043] The specific experimental method is as follows:

[0044] 1. Materials, media and solutions

[0045] (1) Material

[0046] Bdellovibrio: BDM01.

[0047] Host bacteria: Bacillus subtilis GIMT1.135, purchased from the Culture Collection Center of Guangdong Institute of Microbiology.

[0048] Water samples containing Artemia saline: collected from Penglai Mozhikou Marine Aquaculture Plant in Shandong Province, water samples containing San Francisco Bay Artemia (Artemia franciscana) aquaculture water, after observation and counting, the number of Artemia saline was about At 2 / mL.

[0049] (2) culture medium

[0050] 2216E Zobel Marine Bacteria Medium: Sea Crystal 30g, Peptone 5g, Yeast Extract 1g, Ferric Phosphate 0.01g, Distilled Water 1000mL, pH7.6~7.8, Agar 15g. After sterilizing at 121°C and 0.1MPa for 20 minutes, pour it into a plate and save it for later use.

[0051] TCBS agar medium (Vibrio selective medium): 89g of TCBS agar, add 1000mL of distilled water, sti...

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Abstract

The invention discloses a method for controlling the total count of bacteria carried by artemia. The method comprises the following steps: preparing a Bdellovibrio sp. diluent of which the concentration is 2*10<2>-2*10<5>PFU / mL, and mixing the Bdellovibrio sp. diluent with a mixed solution of sodium glutamate and cane sugar in a volume ratio of 1:1 to obtain a Bdellovibrio sp. preparation; and mixing the Bdellovibrio sp. preparation with a pretreated artemia-containing water sample. The method provided by the invention is simple and feasible, and is suitable for industrial batch production. The contained sodium glutamate and cane sugar do not have any toxic or side effect on water bodies and the artemia, and do not cause pollution to the water bodies or death of beneficial aquatic plankton such as the artemia. Through the method disclosed by the invention, the total count of the bacteria carried by the artemia can be effectively controlled within 102-103cfu / mL in 9 hours, and the regeneration of the bacteria is slowed down for a long period of time.

Description

technical field [0001] The invention belongs to the technical field of Bdellovibrio, and in particular relates to a method for controlling the number of longitudinal bacteria carried by Artemia. Background technique [0002] Artemia belongs to the crustacean subphylum Branchiopoda, also known as saltwater Artemia. The body length of Artemia is about 1-1.3cm, and the body length of the large Artemia can reach 1.5cm. Artemia is also divided into two sexes. In terms of size, the female is larger than the female. When swimming, it is backstroke (belly facing upwards). The color of the body can change with the salinity of the water body. Artemia mainly live in high saline water bodies with high salinity, distributed in inland salt lakes and coastal salt pans. There are more than 300 Artemia habitats found in the world. The optimum salinity is about 30‰~100‰, and when the salinity is high, the insect body is reddish brown. The insect body can be divided into three parts: head,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00C02F3/34A61K35/74C12N1/20C12R1/01A61P31/04
CPCY02A40/81
Inventor 蔡俊鹏郭衍彪
Owner SOUTH CHINA UNIV OF TECH
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