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Aspergillus terreus ZRV2011F5 and application thereof

A ZRV2011F5, Aspergillus terreus technology, applied in fungi, microorganisms, fermentation and other directions, to achieve high inhibitory effect, reduce cholesterol synthesis, inhibit arteriosclerosis

Active Publication Date: 2014-11-12
浙江五味和食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, natural compounds with sEH inhibitory effects have not been reported yet.

Method used

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  • Aspergillus terreus ZRV2011F5 and application thereof
  • Aspergillus terreus ZRV2011F5 and application thereof
  • Aspergillus terreus ZRV2011F5 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028]Example 1. Isolation and identification of bacterial strain ZRV2011F5 and detection of monacolin K

[0029] 1. Isolation and purification of strains

[0030] Filamentous fungi were isolated from rice vinegar solid-state fermented vinegar mash (cake from rice saccharification stage) using potato dextrose agar (PDA) medium. Use sterile water to prepare vinegar mash into a suspension with a mass concentration of 20%, further dilute it with sterile water 10 times, 100 times and 1000 times, spread it on tiger red PDA plates respectively, and incubate at 25°C-30°C for 5 days , From the plate that can pick out a single colony, select colonies of different forms and streak culture on the PDA plate, culture at 25°C-30°C for 3-5 days, pick a single colony from the plate for streak culture, and save it on the PDA slant . Pick colonies from the PDA slant and inoculate them on the PDA plate for streak culture, culture at 25-30°C for 3 days, repeat the streak culture twice for furth...

Embodiment 2

[0042] Embodiment 2. Aspergillus terreus ZRV2011F5 produces monacolin K

[0043] Inoculate Aspergillus terreus ZRV2011F5 on the PDA slant, culture at 25-30°C for 5 days to obtain the slant; pick the slant from the slant and inoculate it into M6 medium, cultivate it at 25-30°C for 13 days, take the culture solution and freeze-dry it , add 1 times the volume of the culture medium with a volume concentration of 90% methanol aqueous solution, vortex and mix well, sonicate in an ultrasonic cleaner for 10 sec, and stand at 4°C for 12 hours to obtain a methanol extract, centrifuge to take the supernatant, and use The method described in step 3 of Example 1 was detected by HPLC.

[0044] In the M6 ​​medium, the open-ring acid form monacolin K was detected, and the retention time was 20.02min ( image 3 Middle B), the content reached 40 μg / ml.

[0045] The final concentration composition of M6 medium is as follows: glycerol 110g / L, glucose 30g / L, soybean meal 50g / L, peptone 8g / L, sod...

Embodiment 3

[0047] Change the composition of the M6 ​​medium in Example 2 to:

[0048] Glycerin 70g / L, glucose 30g / L, soybean meal 30g / L, peptone 8g / L, sodium nitrate 2g / L, magnesium sulfate 1g / L, olive oil 5g / L, solvent is deionized water, pH value is natural.

[0049] Other operation is with embodiment 2, and the result sees Figure 4 shown. The results showed that the open acid form monacolin K was detected in the M6 ​​medium ( Figure 4 In B and C), the content is 115 μg / ml. In combination with Example 2, it is shown that Aspergillus terreus ZRV2011F5 can produce monacolin K in a wider range of media, and it is possible to obtain the possibility of optimizing production conditions.

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Abstract

The invention discloses a new bacterial strain, namely aspergillus terreus ZRV2011F5, and the application of aspergillus terreus ZRV2011F5. The aspergillus terreus ZRV2011F5 is preserved in the general microorganism center of the China Committee for Culture Collection of Microorganisms. The preservation serial number is CGMCC NO.8746. The preservation date is January 20th, 2014. The preservation address is NO.3, building 1, Beichen West Road, Chaoyang District, Beijing, namely the Institute of Microbiology Chinese Academy of Sciences. By the adoption of aspergillus terreus ZRV2011F5, monacolinK can be produced, the content of aspergillus terreus ZRV2011F5 secreted into a culture medium is 116 micrograms per milliliter. In addition, the metabolite of aspergillus terreus ZRV2011F5 has inhibitory activity for two enzymes of sEH, and namely, the inhibitory function on hydrolase and phosphatase is high. The inhibitory rates are 72% and 93% when the additive amount of aspergillus terreus ZRV2011F5 is 5%.

Description

(1) Technical field [0001] The present invention relates to a filamentous fungus capable of producing monacolin K (monacolin K) from a rice vinegar fermentation process, in particular to Aspergillus terreus ( Aspergillus terreus) ZRV2011F5 and its application. (2) Background technology [0002] Statins are excellent drugs that inhibit cholesterol synthesis, can effectively reduce LDL-cholesterol levels, and increase HDL-cholesterol levels, effectively preventing the formation of atherosclerotic plaques, with global sales reaching more than 14 billion US dollars , ranking third in therapeutic drugs (Li Shiweng and Feng Lin, Pharmaceutical Biotechnology, 2011, 18(6), 536-68). Monacolin K, also known as lovastatin, was discovered from Monascus ruber and Aspergillus terreus in 1979 and 1980, and was approved by FDA in 1987. The industrial strain of monacolin K is A. terreus derived from soil. [0003] Monacolin K has been proven to have a variety of pharmacological effects. I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P17/06C12R1/66
Inventor 朱立颖冯纬王欣周利南
Owner 浙江五味和食品有限公司
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