Application of Chelatococcus daeguensis in wastewater treatment

A technology for Billiardella chelatum and wastewater treatment, which is used in metallurgical wastewater treatment, biological water/sewage treatment, water/sludge/sewage treatment, etc. It can solve problems such as compound pollution of nitrogen and hexavalent chromium in water body, and save process. Cost, solving the inconsistency of environmental conditions, and the effect of a wide range of adaptations

Active Publication Date: 2014-11-26
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a strain of Chelatococcus daeguensis capable of synchronous denitrificat...

Method used

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  • Application of Chelatococcus daeguensis in wastewater treatment
  • Application of Chelatococcus daeguensis in wastewater treatment
  • Application of Chelatococcus daeguensis in wastewater treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Chelatococcus daeguensis TAD1 for leachate treatment in landfill

[0032] The bacteria was used to treat the leachate of a landfill site, and the water quality test data of the leachate was COD3104mg / L, NO 3 -N 5.45mg / L, NH 4 -N 1129mg / L, pH 7.5~9, Cr(VI) 6.32mg / L, water temperature 45~50℃. The bacterium is added in the biological aerated tank by 0.2% of the bacterial dosage (dry weight of the thalline is 8g / L), the fixed hydraulic retention time HRT=24h, the flow rate of the gas flowmeter of the aeration system is 3L / min, and the treatment After water quality is COD 135.21mg / L, NO 3 -N 0mg / L, NH 4 -N 12.09mg / L, Cr(VI) 0mg / L corresponding pollutant removal rate is 95.64%, 100.00%, 98.92%, 100.00%. Compared with the control group without adding the bacteria, COD, NO 3 -N, NH 4 The removal rates of -N and Cr(VI) were 61%, 55%, 79% and 20%, respectively. Therefore, the addition of the bacteria significantly improved the denitrification capacity of the system.

Embodiment 2

[0034] Chelatococcus daeguensis TAD1 for industrial wastewater treatment

[0035] The bacteria is used to treat the leachate of a company's industrial chromium slag. The concentration of hexavalent chromium in the leachate is about 105.6mg / L, and the initial pH value is adjusted to 7-7.5. The electroplating wastewater is introduced into the biochemical treatment pool, and 10% Add 3% waste molasses to the expanded cultured TAD1 bacterial liquid, stir the mixed solution at 45-50°C, and regularly detect the concentration of hexavalent chromium. After 24h, 48h, and 72h, the reduction rates of hexavalent chromium reached 63.57%, 63.57%, respectively. 84.21%, 100%.

Embodiment 3

[0037] Effect of Nitrogen Source on Denitrification and Cr(VI) Removal by Bacterial Strain

[0038] Take the strain TAD1 stored in the laboratory glycerol tube and inoculate it into 50mL of sterilized seed medium (each L medium contains 1g peptone, 0.5g yeast extract powder, 0.5g NaCl, pH=7), at a temperature of 50°C and a rotation speed of Under the condition of 180r / min, constant temperature shaking and activating culture for 12h, then pipette 10mL of expanded culture bacteria solution to inoculate in 100mL sterilized denitrification medium containing Cr(VI) concentration of 5mg / L (each L medium contains 9.4g C 4 h 4 Na 2 o 4 , 1.0g KNO 3 , 7.9 g Na 2 HPO 4 , 1.5g KH 2 PO 4 , 0.1gMgSO 4 ·7H 2 O, trace elements 1mL, pH=7), where Cr(VI) is K 2 Cr 2 o 7 At the same time, set up a control experiment, keep the C / N ratio and other components of the medium unchanged, change the nitrogen source to sodium nitrite and ammonium chloride, and cultivate for 24 hours under the...

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Abstract

The invention discloses an application of Chelatococcus daeguensis in wastewater treatment. The strain is Chelatococcus daeguensis TAD1 with the collection number of CGMCC NO.5226. The Chelatococcus daeguensis CGMCC 5226 is used for synchronously removing nitrogen and hexavalent chromium in wastewater. The strain has broad spectrum activity in adaptability to temperature and has high removal efficiency at 30-50 DEGC. With the strain, Cr (VI) is reduced to Cr (III) with little toxicity, and part of nitrogen is reduced to N2 or N2O. Thus, the strain can be used to realize synchronous removal of nitrogen, Cr (VI) and carbon, and has a wide application prospect in polluted water body remediation.

Description

technical field [0001] The invention relates to the application of a strain of Chelatococcus daeguensis TAD1 in biological denitrification and removal of Cr(VI) from wastewater. The strain can perform aerobic denitrification denitrification function under different conditions, and complete the removal of Cr(VI) while denitrification, so as to achieve the purpose of synchronous denitrification and removal of hexavalent chromium in wastewater. Background technique [0002] With the increase of people's demand for water and the aggravation of water pollution, the crisis of water resources has become a worldwide problem. The total reserves of water resources in China ranks fourth in the world, and the per capita water resources are only 1 / 4 of the world average, ranking 110th. China is one of the 13 water-poor countries in the world. The sedimentation of nitrogen oxides in industrial waste gas, the migration and infiltration of solid waste, the unreasonable discharge of wastewa...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12N1/20C02F103/16C12R1/01
Inventor 黄少斌吉海鹏张永清
Owner SOUTH CHINA UNIV OF TECH
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