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Application of MicroRNA-433 in preparation of medicament for treating renal failure complicated hypertension, and medicament

A mir-433, high blood pressure technology, applied in gene therapy, drug combinations, pharmaceutical formulations, etc., can solve the problems of not producing effective results, promoting nephritis, impairing the anti-inflammatory properties of TGF-beta, and inhibiting renal fibrosis. the effect of

Inactive Publication Date: 2014-12-03
SHENZHEN RES INST THE CHINESE UNIV OF HONG KONG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, after in-depth investigation, it was found that the use of these strategies did not produce the expected effective results.
This may be due to the fact that blockade of the TGF-beta signaling upstream pathway impairs the potent anti-inflammatory properties of TGF-beta and subsequently promotes renal inflammation
On the other hand, crosstalk of intracellular Smad pathways hinders strategies targeting TGF-beta and TGF-beta receptor kinases

Method used

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  • Application of MicroRNA-433 in preparation of medicament for treating renal failure complicated hypertension, and medicament
  • Application of MicroRNA-433 in preparation of medicament for treating renal failure complicated hypertension, and medicament
  • Application of MicroRNA-433 in preparation of medicament for treating renal failure complicated hypertension, and medicament

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Embodiment Construction

[0030] Embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings. It should be emphasized that the following description is only exemplary and not intended to limit the scope of the invention and its application.

[0031] In the embodiment of the present invention, it is preferable to transfect the shRNA plasmid inhibiting miR-433 into the kidney by ultrasonic microvesicle plasmid transfection technology, so as to down-regulate the expression of miR-433 in kidney cells. The good effect of miR-433 in the treatment of chronic hypertensive kidney disease can be detected. Since the microRNA regulated by TGF-beta plays an important role in the process of TGF-beta-induced fibrosis, we can specifically target TGF-beta downstream microRNA to inhibit the downstream pathway of TGF-beta, thereby controlling TGF-beta beta-induced fibrosis without compromising the anti-inflammatory properties of TGF-beta would be a better option f...

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Abstract

The invention discloses an application of a substance of inhibiting miR-433 in preparation of a medicament for treating renal failure complicated hypertension, wherein the medicament is prepared from knockdown microRNA-433 plasmids. The invention also discloses a medicament for treating renal failure complicated hypertension, and the medicament contains the knockdown microRNA-433 plasmids. The medicament provided by the invention can be applied to realization of a novel, safe, effective and controllable gene therapy, so as to inhibit TGF-beta-mediated renal fibrosis activated from an AngII signal.

Description

technical field [0001] The invention relates to the application of MicroRNA-433 in the preparation of medicines for treating hypertension and renal failure and the medicines thereof. Background technique [0002] Hypertensive nephropathy is one of the most important causes of end-stage renal disease, and hypertension is also the most common complication of chronic renal failure. [0003] In chronic hypertensive kidney disease, due to the decrease of glomerular filtration rate, the kidney itself will produce type II angiotensin II (AngII), and Ang II will constrict efferent arterioles and cause glomerulus pressure to rise To compensate for the decline in glomerular filtration rate. At the same time, AngII causes renal fibrosis by activating TGF-beta / Smad signaling. Finally, renal fibrosis leads to end-stage renal failure. Since TGF-beta / Smad signaling regulates the expression of extracellular matrix in kidney diseases, targeting TGF -beta signaling is an attractive therapeu...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K49/22A61P13/12A61P9/12
Inventor 钟志刚
Owner SHENZHEN RES INST THE CHINESE UNIV OF HONG KONG