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New medical applications of caffeol

A kind of cafestol and drug technology, applied in the application field of cafestol in the preparation of drugs for preventing or treating renal fibrosis

Pending Publication Date: 2022-02-11
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Cafestol is an organic compound with a molecular formula of C20H28O3. It is a diterpene compound in coffee. There is no report about its inhibitory effect on renal fibrosis.

Method used

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  • New medical applications of caffeol
  • New medical applications of caffeol
  • New medical applications of caffeol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Caffeine inhibits renal fibrosis in mice induced by unilateral ureteral occlusion (UUO)

[0044] 1. Animals

[0045] SPF grade BALB / C male mice were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.

[0046] 2. Experimental drugs and reagents

[0047] Cafestol (sigma), phenobarbital sodium (LUPI), 4% paraformaldehyde (Beijing Regen Biotechnology Co., Ltd.), OCT tissue embedding agent (SAKURA), immunostaining primary antibody dilution (Beyond), immune Fluorescence staining secondary antibody diluent (Biyuntian), modified Masson trichrome staining solution (Solarbio), neutral gum (Beijing Solarbio Technology Co., Ltd.), RIPA lysate (medium) (Biyuntian), phosphatase inhibitor tablets (Roche), protease inhibitor (Kangwei Century), 5×SDS-PAGE loading buffer (Kangrun Biotechnology Co., Ltd.), 4×SDS-PAGE separating gel buffer (Solarbio), 4×SDS-PAGE stacking gel buffer (Solarbio), 30% acrylamide (29:1) (Solarbio), PAGE gel accelerator (S...

Embodiment 2

[0056] Example 2 Cafestol can significantly increase the expression of phosphorylated YAP, reduce the amount of YAP / TAZ, and inhibit the Hippo-YAP signaling pathway.

[0057] The Western blotting method in this embodiment is carried out using common experimental procedures in the field.

[0058] In-cell Western is a high-throughput method for rapid detection of intracellular proteins, which can be directly performed on cells on cell culture plates (such as 96-well plates) based on the principle of antigen-antibody binding, using specific antibodies for target proteins and near-infrared Fluorescently labeled secondary antibodies for quantitative analysis of target proteins.

[0059] The experimental steps are as follows:

[0060] After the cell culture in the 96-well plate was completed, the culture medium was discarded, and the residual medium was washed with PBS. Fix with 4% paraformaldehyde for 10 min and wash with PBS 3 times. Permeabilize with 0.3% Triton X-100 for 15 m...

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PUM

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Abstract

The invention provides applications of caffeol in the preparation of a medicine for preventing and / or treating renal tissue fibrosis. The caffeol is applied as follows: a1, preparing of the medicine for preventing and / or treating renal fibrosis; a2, preventing and / or treating renal tissue fibrosis; a3, inhibiting a Hippo-YAP signal channel; and a4, establishing a renal tissue fibrosis animal medical model.

Description

technical field [0001] The invention belongs to the technical field of natural product medicine, and in particular relates to the application of cafestol in the preparation of drugs for preventing or treating renal fibrosis. Background technique [0002] Chronic kidney disease (chronic kidney disease) has a global prevalence of 10-12%, and it is increasing year by year. Chronic glomerulonephritis, hypertensive nephropathy, diabetic nephropathy, renal interstitial disease, renal tubular disease, congenital Acquired kidney disease and irrational use of nephrotoxic drugs can cause chronic kidney disease, which eventually progresses to end-stage renal disease and becomes a high-risk factor for death. Renal fibrosis (renal fibrosis) is a key and common pathological change in the early stages of chronic kidney disease caused by various causes to end-stage renal failure, which seriously affects kidney function. The etiology and pathogenesis of renal fibrosis are very complicated, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/343A61P13/12A01K67/027
CPCA61K31/343A61K9/0053A61P13/12A01K67/027A01K2207/20A01K2227/105A01K2267/03
Inventor 王帅玉董彦君毛艺融任亚琪范开郝智慧
Owner CHINA AGRI UNIV