Fingerprint map detection method for ganmaoling granules

A fingerprint and detection method technology, applied in the field of detection of traditional Chinese medicine preparations, can solve the problems of not being able to systematically and comprehensively reflect the internal quality of Ganmaoling granules, the type of ingredients and the unstable content, etc., and achieve good reproducibility, quick operation, and high results. Objective and accurate effect

Active Publication Date: 2014-12-03
CHINA RESOURCES SANJIU MEDICAL & PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the quality of traditional Chinese medicinal materials is affected by many aspects such as the place of origin, climate, season, etc., the type and content of the ingredients contained are often unstable. For

Method used

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  • Fingerprint map detection method for ganmaoling granules
  • Fingerprint map detection method for ganmaoling granules
  • Fingerprint map detection method for ganmaoling granules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0112] The fingerprint detection method of Ganmaoling granules described in this embodiment comprises the following steps:

[0113] A. Preparation of the test solution

[0114] Weigh 4.0g of Ganmaoling granule powder, add 25ml of methanol, ultrasonically treat for 30min at a power of 200W, frequency of 59KHz, take it out, let it cool, weigh again, make up the lost weight with methanol, shake well, filter, and measure Take 10ml of the filtrate, evaporate to dryness, dissolve the residue with 50% methanol, put it in a 5ml measuring bottle and set the volume to the mark, shake well, and pass through a 0.45μm microporous membrane to obtain the product;

[0115] B. Preparation of reference substance solution

[0116] Take an appropriate amount of fenugreek, weigh it accurately, add methanol, dissolve and dilute, and make 0.00001g per 1ml of fenugreek, and you get it;

[0117] C. Chromatographic conditions

[0118] Using column Kromasil C 18 (250mm×4.6mm, 5um); with acetonitrile...

Embodiment 2

[0122] The fingerprint detection method of Ganmaoling granules described in this embodiment comprises the following steps:

[0123] A. Preparation of the test solution

[0124] Weigh 3.0g of Ganmaoling granule powder, add 28ml of methanol, ultrasonically treat it for 35min at a power of 200W and a frequency of 59KHz, take it out, let it cool, weigh again, make up the lost weight with methanol, shake well, filter, and measure Take 8ml of the filtrate, evaporate to dryness, dissolve the residue with 50% methanol, put it in a 5ml measuring bottle and set the volume to the mark, shake well, and pass through a 0.45μm microporous membrane to obtain the product;

[0125] B. Preparation of reference substance solution

[0126] Take an appropriate amount of fenugreek, accurately weigh it, add methanol to dissolve and dilute, and make 0.00001g per 1ml of fenugreek, and you get it;

[0127] C. Chromatographic conditions

[0128] Using column Kromasil C 18 (250mm×4.6mm, 5um); with ace...

Embodiment 3

[0132] The fingerprint detection method of Ganmaoling granules described in this embodiment comprises the following steps:

[0133] A. Preparation of the test solution

[0134] Weigh 5.0g of Ganmaoling granule powder, add 22ml of methanol, ultrasonically treat for 25min at a power of 200W, frequency of 59KHz, take it out, let it cool, weigh again, make up the lost weight with methanol, shake well, filter, and measure Take 12ml of the filtrate, evaporate to dryness, dissolve the residue with 50% methanol, put it in a 5ml measuring bottle and set the volume to the mark, shake well, and pass through a 0.45μm microporous membrane to obtain the product;

[0135] B. Preparation of reference substance solution

[0136] Take an appropriate amount of fenugreek, accurately weigh it, add methanol to dissolve and dilute, and make 0.00001 parts by weight per 1 volume part of fengalin, to obtain final product;

[0137] C. Chromatographic conditions

[0138] Using column Kromasil C 18(25...

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Abstract

The invention belongs to the field of traditional Chinese medicinal preparation detection, and in particular relates to a fingerprint map detection method for ganmaoling granules. According to the method disclosed by the invention, traditional Chinese medicinal components, including linarin, in the ganmaoling granules are detected by utilizing a high-performance liquid chromatography; quality information of the ganmaoling granules can be reflected comprehensively; the intrinsic quality of the ganmaoling granules can be effectively controlled; moreover, the established method is relatively high in stability, precision and reproducibility; a traditional Chinese medicine chromatographic fingerprint map similarity evaluation system specified by the Pharmacopoeia Committee of China is adopted, so that the operation is quick, and results are objective and accurate.

Description

technical field [0001] The invention belongs to the field of detection of traditional Chinese medicine preparations, and in particular relates to a fingerprint detection method of Ganmaoling granules. Background technique [0002] The composition of traditional Chinese medicine is complex, and the active part is often not a single component. Taking a single component as a quality control index is becoming less and less suitable for the quality control requirements of traditional Chinese medicine. Therefore, the fingerprint technology of traditional Chinese medicine has emerged as the times require. [0003] Traditional Chinese medicine fingerprint technology originates from fingerprint identification, and uses modern information technology and quality analysis methods to comprehensively reflect the types and quantities of chemical components contained in traditional Chinese medicine. For Chinese medicinal materials, fingerprints can be used to identify the authenticity and j...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 李琼娅林丽娜张跃飞马鹏岗
Owner CHINA RESOURCES SANJIU MEDICAL & PHARMA
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