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Reagent freeze-dried microsphere for detecting concentrated carbon dioxide binding force and preparation method of reagent freeze-dried microsphere

A technology for detecting reagents and carbon dioxide, which is applied in the field of freeze-dried microspheres and their preparation, and achieves the effects of solving the problem of difficulty in encapsulation and quantification, easy encapsulation, and uniform size and shape.

Active Publication Date: 2014-12-17
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a concentrated carbon dioxide binding capacity detection reagent freeze-dried microspheres and its preparation method, to solve the problems existing in the prior art liquid and freeze-dried powder carbon dioxide binding capacity detection reagents

Method used

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  • Reagent freeze-dried microsphere for detecting concentrated carbon dioxide binding force and preparation method of reagent freeze-dried microsphere
  • Reagent freeze-dried microsphere for detecting concentrated carbon dioxide binding force and preparation method of reagent freeze-dried microsphere
  • Reagent freeze-dried microsphere for detecting concentrated carbon dioxide binding force and preparation method of reagent freeze-dried microsphere

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preparation example Construction

[0044] A method for preparing concentrated carbon dioxide binding capacity detection reagent freeze-dried microspheres, comprising the following steps:

[0045] (1), preparation of carbon dioxide binding capacity detection reagent;

[0046] (2), add lyoprotectant and plasticizer, filter to remove insoluble matter after fully dissolving, vacuum degassing removes air bubble;

[0047] (3) Micro-droplets are prepared by using a liquid separation system that can be accurately quantified, and the droplets are dropped into liquid nitrogen, and frozen reagent microspheres are formed after being cooled by liquid nitrogen;

[0048] (4) Transfer the frozen reagent microspheres into a lyophilizer to freeze-dry to prepare the reagent lyophilized microspheres.

[0049] The volume of the droplet in step (3) is precisely controlled by a liquid separation system, the size of the droplet is 2-10ul, and the size error is within 2%.

[0050] The carbon dioxide binding force detection reagent fr...

Embodiment 1

[0066] Table 1. Composition of carbon dioxide binding capacity detection reagents

[0067] Element

Embodiment 2

[0069] Table 2. Composition of Carbon Dioxide Binding Ability Detection Reagents

[0070] Element

concentration

Tris buffer

90mM

Sodium chloride

450mM

carbonic anhydrase

15KU / L

Phenol red

420mg / L

bromothymol blue

180mg / L

Methyl hydroxymethylbenzoate

1g / L

polyethylene glycol 4000

25g / L

polyethylene glycol 8000

22g / L

sucrose

15g / L

Surfactant Trion X-100

3g / L

[0071] The surfactant TrionX-100 in the carbon dioxide binding force detection reagent can effectively control the generation of bubbles during the dissolution of the reagent microspheres; the freeze-drying protection agent in the reagent can use sucrose, trehalose, polyethylene glycol, bovine serum albumin , polyvinylpyrrolidone, mannitol, and dextran, the content of which can be determined according to the morphology and solubility of the reagent freeze-dried microspheres.

[0072] (2) Prepa...

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Abstract

The invention discloses a reagent freeze-dried microsphere for detecting concentrated carbon dioxide binding force and a preparation method of the reagent freeze-dried microsphere. The reagent freeze-dried microsphere comprises a buffer solution, carbonic anhydrase, phenol red, bromothymol blue, a preservative, trehalose, polyethylene glycol, TrionX-100 and the like. The preparation process of the reagent freeze-dried microsphere mainly comprises the following steps: (1) preparing a solution, filtering and degassing; (2) preparing microdroplets by using an accurate quantifying liquid separation system, and dropping the microdroplets into liquid nitrogen to prepare frozen spheres; and (3) transferring the frozen spheres to a freeze dryer to freeze and dry, thereby obtaining the freeze-dried reagent microsphere. A reagent for detecting carbon dioxide binding force disclosed by the invention is a spherical particle freeze-dried reagent, and can be pre-packaged into a detection chip. Compared with an existing liquid detection reagent, the reagent has the advantages that stable preservation and transportation at room temperature can be achieved; and compared with a freeze-dried powder reagent, the reagent has the advantages of being accurate to quantify, convenient to package and the like, and can be suitable for a POCT biochemical analyzer introducing a microfluidic chip technology.

Description

technical field [0001] The invention relates to the field of freeze-dried microspheres and the preparation thereof, in particular to a concentrated carbon dioxide binding force detection reagent freeze-dried microspheres and a preparation method thereof. Background technique [0002] Carbon dioxide binding capacity refers to the content of carbon dioxide contained in bicarbonate in plasma. Respiratory acidosis: respiratory muscle paralysis, bronchiectasis, pneumothorax and airway obstruction; metabolic alkalosis: vomiting, adrenal hyperfunction, potassium deficiency and taking too much alkaline drugs can cause acid-base poisoning, thereby reducing the ability of carbon dioxide to combine Increased; metabolic acidosis, such as uremia, shock, diabetic ketosis, severe diarrhea and dehydration; respiratory alkalosis, such as respiratory center excitement and rapid breathing, etc. will cause a decrease in carbon dioxide binding capacity. Therefore, the determination of the carbo...

Claims

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Application Information

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IPC IPC(8): G01N33/52
CPCG01N33/52
Inventor 张涛黎海文周武平刘聪蒋克明张志强
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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