A kind of microalgae cultivation method

A cultivation method and microalgae technology, applied in the field of microalgae cultivation, can solve the problems affecting the increase of microalgae cultivation output, reducing biomass accumulation, and consuming microalgae energy, so as to reduce ineffective respiration, reduce biomass consumption, Reduce the effect of photorespiration

Active Publication Date: 2017-12-19
ENN SCI & TECH DEV
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Problems solved by technology

[0004] However, the oxygen brought by the aeration process dissolves in the culture system, which promotes the respiration and photorespiration of the microalgae, consumes the energy generated by the photosynthesis of the microalgae, and reduces the accumulation of biomass, thus affecting the microalgae culture. Yield increase

Method used

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  • A kind of microalgae cultivation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0120] A 10cm plate reactor is used to cultivate Nannochloropsis, the suitable pH range for its growth is 6-8, the cultivation scale is 15L, the ventilation required for the cultivation system is 0.3vvm, and the gas ventilation rate is set as 0.27m 3 / h.

[0121] Test group one:

[0122] During the light period, the mixed gas of 3% carbon dioxide and 97% nitrogen of the required ventilation is added, and the pH range of the microalgae culture in the culture system is between 6.5 and 7.5. During the growth process, this condition meets the requirements of the microalgae cells for growth. need;

[0123] During the non-light period, 1% carbon dioxide and 99% air are added to the required ventilation, and the pH range of the culture system is between 7 and 8. During the growth process, this condition meets the growth needs of microalgae cells.

[0124] Several batches were continuously cultivated in this way, and the cultivation period of each batch was 7 days. After each batch...

Embodiment 2

[0135] A 3cm tubular reactor is used to cultivate Chlorella primitive, the suitable pH range for its growth is 6-8, the cultivation scale is 800mL, the ventilation required for the cultivation system is 1vvm, and the gas ventilation rate is set to 0.048 m 3 / h.

[0136] test group:

[0137] During the light period, 5% carbon dioxide and 95% nitrogen are added to the required ventilation, and the pH range of the microalgae culture in the culture system is between 6 and 7. During the growth process, this condition meets the growth needs of the microalgae cells;

[0138] During the non-illumination period, the dissolved oxygen electrode is used to detect the oxygen consumption rate of the microalgae cells, and the required oxygen amount is determined according to the oxygen consumption rate, and then the required air amount is calculated, and the proportion of air added in the required ventilation amount is determined. The test result of oxygen consumption rate is 0.02g oxygen / ...

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Abstract

The invention provides a microalgae cultivation method, which relates to the technical field of microalgae cultivation, and can reduce the consumption of biomass produced by the photosynthesis of the microalgae, improve the accumulation efficiency of biomass, and promote the improvement of the production of microalgae cultivation. The method for cultivating microalgae disclosed by the invention comprises: during the light period, feeding a mixed gas of carbon dioxide and a stable gas into the culture system; and during the non-light period, feeding aerobic gas into the culture system. The microalgae cultivation method disclosed by the invention is suitable for the microalgae cultivation process.

Description

technical field [0001] The invention relates to the technical field of microalgae cultivation, in particular to a microalgae cultivation method. Background technique [0002] Microalgae are a kind of autotrophic plants widely distributed on land and ocean, rich in nutrients and high in photosynthetic utilization. The polysaccharides, proteins, pigments, etc. produced by cell metabolism make them widely used in food, medicine, genetic engineering, liquid fuel and other fields. It has a good development prospect. [0003] At present, in the process of microalgae cultivation, the method of adding air and carbon dioxide mixture to the cultivation system is generally used for cultivation. Among them, the air is mainly used to meet the mixing effect produced by aeration, and the carbon dioxide is mainly used to support the normal growth of microalgae. Provide carbon fixation sources and adjust the pH value of the breeding system, etc. [0004] However, the oxygen brought by the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12
Inventor 冯倩耿金峰杨巧利马欣欣杨建强张凯王冰
Owner ENN SCI & TECH DEV
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