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A method for rapidly detecting the adsorption performance of adsorbent

A kind of adsorbent, one-time technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of cumbersome experimental process, long detection cycle, peak shape tailing, etc., to improve detection efficiency, shorten quantitative and qualitative analysis, good peak shape

Active Publication Date: 2016-08-31
JAFRON BIOMEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The experimental process is cumbersome and consumes a lot of samples, especially for the research and development process, the sample volume of trial production is small and precious, and the cost of separate multiple experiments is high, resulting in great waste
At the same time, it takes a long time to complete the entire inspection cycle, which is not conducive to rapid evaluation of product performance and cannot meet the needs of product development progress.
On the other hand, there are small and medium molecules and various metabolites in the blood at the same time. The separate detection of the three items of creatinine cannot reflect the actual adsorption effect of the adsorbent, and there is a large deviation from the real situation, so it is difficult to play a role in the development of technology. positive impetus
[0004] Ordinary high-performance liquid chromatography cannot achieve rapid and effective separation of the three substances of creatinine, especially for creatinine with high polarity. The usual chromatographic methods have long retention time, severe peak tailing, and additional competitive amines such as Triethylamine to improve peak shape, it is difficult to achieve effective separation of creatinine, sodium pentobarbital and vitamin B12 at the same time

Method used

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  • A method for rapidly detecting the adsorption performance of adsorbent
  • A method for rapidly detecting the adsorption performance of adsorbent
  • A method for rapidly detecting the adsorption performance of adsorbent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Sodium pentobarbital, creatinine and vitamin B 12 separation of the mixture

[0047] (1) Sample preparation

[0048] Formulated Sodium Pentobarbital, Creatinine and B Vitamins 12 Solution and the mixed solution of the three, wherein, in the mixed solution, pentobarbital sodium is 80mg / L, creatinine is 35mg / L, vitamin B 12 25mg / L;

[0049] (2) Liquid phase separation

[0050] The ACQUITY ultra-high performance liquid chromatography (ACQUITY UPLC) of Waters was used to separate and detect the samples, and the specific conditions were as follows:

[0051] Column: ACQUITY UPLC BEH130C18 column, 2.1mm×50mm, 1.7μm

[0052] Detector: UV detector

[0053] Detection wavelength: 200-400nm

[0054] Column temperature: 40°C

[0055] Mobile phase: methanol as mobile phase A, water as mobile phase B, gradient elution

[0056] Flow rate: 0.3ml / min

[0057] Gradient elution program:

[0058] time (min)

Mobile phase A%

Mobile phase B%

0

10...

Embodiment 2

[0061]Example 2 The adsorption rate, concentration and linear fitting degree of creatinine three compound gradient concentration

[0062] (1) Preparation of control samples

[0063] The present embodiment adopts pentobarbital sodium as 80mg / L, creatinine 35mg / L, vitamin B 12 Prepare a mixed solution of three items of creatinine at 25 mg / L, which is recorded as solution S1. Take four parts of solution S1 and dilute them by 4 times, 8 times, 16 times, and 32 times respectively. The corresponding diluted solutions are respectively recorded as solutions S4 and S8. , S16 and S32. The dilution of different multiples is to prove the accuracy of the method at different concentrations, and the peak area under different concentrations can be fitted, and its fitting constant reaches 0.999 (the maximum is 1), which shows that the method can be used at the concentration of the experiment. The detection results within the range are highly accurate and consistent, and can be compared horiz...

Embodiment 3

[0080] Example 3 Detect the adsorption rate of the three items of creatinine after the adsorbent completes the adsorption experiment

[0081] (1) Preparation of control samples

[0082] Formulated with Sodium Pentobarbital, Creatinine and B Vitamins 12 Solution S1, pentobarbital sodium 80mg / L, creatinine 35mg / L, vitamin B 12 It is 25mg / L.

[0083] (2) Preparation of test samples

[0084] Take 125ml of solution S and place it in a 50ml Erlenmeyer flask with a stopper, weigh 1.0g of dry adsorbent (wet state can be converted into dry weight) and put it into the bottle, place it in an environment of 37°C±1°C for 1 hour with constant temperature oscillation and adsorption. Take the supernatant and call it solution A.

[0085] (3) Liquid chromatography separation

[0086] Adopt Waters (Water's) chromatographic system to carry out chromatographic separation of solution S and solution A, adopt ultraviolet detector to detect, specific conditions are as follows:

[0087] Column: A...

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Abstract

The invention provides a method for quickly detecting the adsorption performance of an adsorbent, which uses an ultra-high performance liquid chromatography system to detect related substances, adopts an ACQUITY UPLC BEH130C18 chromatographic column, an ultraviolet detector, uses acetonitrile or methanol as the mobile phase A, and uses water For mobile phase B, gradient elution. The invention achieves baseline separation within five minutes and has a good peak shape, which is convenient for further quantitative and qualitative analysis, greatly shortens the detection time, improves the detection efficiency, and can perform quantitative and qualitative analysis on the three items of creatinine at one time, and can be detected at one time The adsorption rate and adsorption capacity of the adsorbent to the three items of creatinine can reflect the adsorption performance of the adsorbent quickly, efficiently and accurately. At the same time, the present invention realizes the adsorption experiment under the condition that the three substances of creatinine exist at the same time, and the results can be More realistically reflect the adsorption performance of the adsorbent on various substances in the blood.

Description

technical field [0001] The invention relates to a compound detection method, in particular to a method for rapidly detecting the adsorption performance of an adsorbent. Background technique [0002] The three items of creatinine are composed of pentobarbital sodium, vitamin B 12 and creatinine, which represent small molecular substances, medium molecular weight substances and metabolic substances in the body, respectively. The three adsorption properties of creatinine respectively represent the adsorption capacity of small and medium molecules and metabolites, and are comprehensive indicators for evaluating the performance of hemoperfusion adsorbents. [0003] The current detection method for the performance of the hemoperfusion adsorbent is to use these three substances to detect the adsorption of the adsorbent to it, and use an ultraviolet spectrophotometer to separately detect the ultraviolet absorption spectra of the three compounds, and formulate standards respectively...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 董凡黄杰辉
Owner JAFRON BIOMEDICAL
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