Method for rapidly detecting adsorption property of adsorbent

A kind of adsorbent, one-time technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of long detection cycle, cumbersome experimental process, peak shape tailing, etc., achieve quantitative and qualitative analysis shortening, improve detection efficiency, good peak shape

Active Publication Date: 2014-12-31
JAFRON BIOMEDICAL
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The experimental process is cumbersome and consumes a lot of samples, especially for the research and development process, the sample volume of trial production is small and precious, and the cost of separate multiple experiments is high, resulting in great waste
At the same time, it takes a long time to complete the entire inspection cycle, which is not conducive to rapid evaluation of product performance and cannot meet the needs of product development progress.
On the other hand, there are small and medium molecules and various metabolites in the blood at the same time. The separate detection of the three items of creatinine cannot reflect the actual adsorption effect of the adsorbent, and there is a large deviation from the real situation, so it is difficult to play a role in the development of technology. positive impetus
[0004] Ordinary high-performance liquid chromatography cannot achieve rapid and effective separation of the three substances of creatinine, especially for creatinine with high polarity. The usual chromatographic methods have long retention time, severe peak tailing, and additional competitive amines such as Triethylamine to improve peak shape, it is difficult to achieve effective separation of creatinine, sodium pentobarbital and vitamin B12 at the same time

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for rapidly detecting adsorption property of adsorbent
  • Method for rapidly detecting adsorption property of adsorbent
  • Method for rapidly detecting adsorption property of adsorbent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Sodium pentobarbital, creatinine and vitamin B 12 separation of the mixture

[0047] (1) Sample preparation

[0048] Formulated Sodium Pentobarbital, Creatinine and B Vitamins 12 Solution and the mixed solution of the three, wherein, in the mixed solution, pentobarbital sodium is 80mg / L, creatinine is 35mg / L, vitamin B 12 25mg / L;

[0049] (2) Liquid phase separation

[0050] The ACQUITY ultra-high performance liquid chromatography (ACQUITY UPLC) of Waters was used to separate and detect the samples, and the specific conditions were as follows:

[0051] Column: ACQUITY UPLC BEH130C18 column, 2.1mm×50mm, 1.7μm

[0052] Detector: UV detector

[0053] Detection wavelength: 200-400nm

[0054] Column temperature: 40°C

[0055] Mobile phase: methanol as mobile phase A, water as mobile phase B, gradient elution

[0056] Flow rate: 0.3ml / min

[0057] Gradient elution program:

[0058] time (min)

Mobile phase A%

Mobile phase B%

0

1...

Embodiment 2

[0061]Example 2 The adsorption rate, concentration and linear fitting degree of creatinine three compound gradient concentration

[0062] (1) Preparation of control samples

[0063] The present embodiment adopts pentobarbital sodium as 80mg / L, creatinine 35mg / L, vitamin B 12 Prepare a mixed solution of three items of creatinine at 25 mg / L, which is recorded as solution S1. Take four parts of solution S1 and dilute them by 4 times, 8 times, 16 times, and 32 times respectively. The corresponding diluted solutions are respectively recorded as solutions S4 and S8. , S16 and S32. The dilution of different multiples is to prove the accuracy of the method at different concentrations, and the peak area under different concentrations can be fitted, and its fitting constant reaches 0.999 (the maximum is 1), which shows that the method can be used at the concentration of the experiment. The detection results within the range are highly accurate and consistent, and can be compared horiz...

Embodiment 3

[0080] Example 3 Detect the adsorption rate of the three items of creatinine after the adsorbent completes the adsorption experiment

[0081] (1) Preparation of control samples

[0082] Formulated with Sodium Pentobarbital, Creatinine and B Vitamins 12 Solution S1, pentobarbital sodium 80mg / L, creatinine 35mg / L, vitamin B 12 It is 25mg / L.

[0083] (2) Preparation of test samples

[0084] Take 125ml of solution S and place it in a 50ml Erlenmeyer flask with a stopper, weigh 1.0g of dry adsorbent (wet state can be converted into dry weight) and put it into the bottle, place it in an environment of 37°C±1°C for 1 hour with constant temperature oscillation and adsorption. Take the supernatant and call it solution A.

[0085] (3) Liquid chromatography separation

[0086] Adopt Waters (Water's) chromatographic system to carry out chromatographic separation of solution S and solution A, adopt ultraviolet detector to detect, specific conditions are as follows:

[0087] Column: A...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for rapidly detecting the adsorption property of an adsorbent. An ultra-high performance liquid chromatography system is adopted to detect relevant substances, ACQUITY UPLC BEH130C18 chromatographic columns and ultraviolet detectors are adopted, and acetonitrile or methanol is taken as flow phase A and water is taken as flow phase B for gradient elution. According to the method, base line separation is achieved within five minutes, the wave form is good, further quantitative and qualitative analysis is facilitated, the detection time is greatly shortened, the detection efficiency is improved, quantitative and qualitative analysis on three indexes of creatinine can be performed at a time, and the adsorption rate and the adsorption amount of the adsorbent to the three indexes of creatinine can be detected at a time, so that the adsorption property of the adsorbent can be rapidly, efficiently and accurately revealed; meanwhile the adsorption experiment can be performed under the condition that three substances of creatinine exist at the same time, and the result can practically reveal the adsorption property of the adsorbent on various substances in blood.

Description

technical field [0001] The invention relates to a compound detection method, in particular to a method for rapidly detecting the adsorption performance of an adsorbent. Background technique [0002] The three items of creatinine are composed of pentobarbital sodium, vitamin B 12 and creatinine, which represent small molecular substances, medium molecular weight substances and metabolic substances in the body, respectively. The three adsorption properties of creatinine respectively represent the adsorption capacity of small and medium molecules and metabolites, and are comprehensive indicators for evaluating the performance of hemoperfusion adsorbents. [0003] The current detection method for the performance of the hemoperfusion adsorbent is to use these three substances to detect the adsorption of the adsorbent to it, and use an ultraviolet spectrophotometer to separately detect the ultraviolet absorption spectra of the three compounds, and formulate standards respectively...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 董凡黄杰辉
Owner JAFRON BIOMEDICAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap