Method for alternately storing trichogramma at intermediate and low temperature in two steps

A technology of low-temperature storage and Trichogramma, applied in animal husbandry, etc., to achieve the effects of reducing production costs, improving storage resistance, and improving control effects

Inactive Publication Date: 2015-01-07
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a two-step medium and low temperature alternate storage method for Trichogramma, which solves the problem of medium and long-term storage of Trichogramma, and can prolong the life of Trichogramma. The storage time of Trichogramma is more than 90 days, which breaks the seasonal limitation of Trichogramma production, changes seasonal production to annual production, and expands production capacity by 2-3 times under the condition of unchanged plant and equipment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Using tussah cocoons as raw materials to produce the pine caterpillar Trichogramma, store them alternately at medium and low temperatures in two steps, and use them in the field in early July to control the first generation of corn borer as an example:

[0020] 1. Production of bee species:

[0021] In mid-December, warm cocoons and moths start to grow moths. After the moths emerge around January 10, take caesarean eggs, and then pick up bees at a ratio of 1:35-40. After picking up bees in a dark room at 23-25°C and RH70-75% for 24 hours , remove bee species, transfer to 20-25°C variable temperature, RH70-75%, L16:D8 conditions to develop to the middle and late stages of larvae, and refrigerate for later use.

[0022] 2. Production of host eggs

[0023] Start hanging cocoons around February 25th, and heat them under the conditions of 18-23°C and RH60-65%. After the moths emerge around March 17th, collect the female moths, take the eggs by caesarean section, wash and sq...

Embodiment 2

[0031] Take tussah silkworm cocoons as raw materials to produce the pine caterpillar Trichogramma, and use it in the field in mid-July to control the first-generation corn borer as an example:

[0032] 2. Production of bee species:

[0033] In the second half of December, warm the cocoons and moths. After the moths emerged around January 20, the eggs were collected by laparotomy, and then the bees were picked up at a ratio of 1:35-40. After picking up the bees in a dark room at 23-25°C and RH70-75% for 24 hours , remove bee species, transfer to 20-25°C variable temperature, RH70-75%, L16:D8 conditions to develop to the middle and late stages of larvae, and refrigerate for later use.

[0034] 2. Production of host eggs

[0035] Start to hang cocoons around March 5th, and heat them under the conditions of 18-23°C and RH60-65%. After the moths emerge around March 25th, collect the female moths, take the eggs by caesarean section, wash and squeeze the green eggs, Disinfect with ...

Embodiment 3

[0043] Taking tussah cocoons as raw materials to produce the pine caterpillar Trichogramma, the field application in August to control the second-generation corn borer is an example:

[0044] 1. Production of bee species:

[0045] In mid-January, the cocoons were warmed up and the moths were raised. After the moths emerged around February 8, the caesarean eggs were collected, and then the bees were picked up at a ratio of 1:35-40. After picking up the bees in a dark room under the conditions of 23-25°C and RH70-75% for 24 hours , remove bee species, transfer to 20-25°C variable temperature, RH70-75%, L16:D8 conditions to develop to the middle and late stages of larvae, and refrigerate for later use.

[0046] 2. Production of host eggs

[0047] Start hanging cocoons around March 26, and heat them under the conditions of 18-23°C and RH60-65%. After the moths emerge around April 15, collect the female moths, take the eggs by caesarean section, and wash and squeeze the green eggs...

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Abstract

The invention provides a method for alternately storing trichogramma at intermediate and low temperature in two steps. After the trichogramma is inoculated, the trichogramma growing to the pre-pupal period is transferred to be stored for more than 70 d under the condition of the temperature of 3+ / -1 DEG C and RH of 70 percent to 85 percent through intermediate-temperature storage and low-temperature storage, and field application can be carried out. According to the new method for storing the trichogramma, the storage resisting capacity of the trichogramma is improved, the storage time of the trichogramma is prolonged, manufacturers can arrange production in advance according to the trichogramma breeding plane, production capacity is enhanced without changing original plant equipment, the utilization rate of the plant equipment and the like is improved, production cost is lowered, and the control effect on injurious insects is improved.

Description

Technical field: [0001] The invention provides a two-step medium and low temperature alternate storage method for Trichogramma, which is a new medium- and long-term storage method for Trichogramma pine caterpillars bred with tussah silkworm eggs as intermediate host eggs, and belongs to the pest biological control technology in the field of agricultural technology . Background technique: [0002] Trichogramma is a kind of egg-parasitic natural enemy insect with the longest research history, the widest application range, the largest control area and the best control effect in modern agricultural and forestry pest biological control. my country's research on Trichogramma began in the early 1930s; after the founding of the People's Republic of China, Sun Yat-sen University and Guangdong Academy of Agricultural Sciences and other units researched and selected castor silkworm eggs to reproduce Trichogramma successfully for the first time, which promoted Trichogramma. In 1964, L...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 张俊杰阮长春朱兴友杜文梅孙光芝臧连生邵玺文王秀梅李天昊
Owner JILIN AGRICULTURAL UNIV
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