Method for alternately storing trichogramma at intermediate and low temperature in two steps
A technology of low-temperature storage and Trichogramma, applied in animal husbandry, etc., to achieve the effects of reducing production costs, improving storage resistance, and improving control effects
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Embodiment 1
[0019] Using tussah cocoons as raw materials to produce the pine caterpillar Trichogramma, store them alternately at medium and low temperatures in two steps, and use them in the field in early July to control the first generation of corn borer as an example:
[0020] 1. Production of bee species:
[0021] In mid-December, warm cocoons and moths start to grow moths. After the moths emerge around January 10, take caesarean eggs, and then pick up bees at a ratio of 1:35-40. After picking up bees in a dark room at 23-25°C and RH70-75% for 24 hours , remove bee species, transfer to 20-25°C variable temperature, RH70-75%, L16:D8 conditions to develop to the middle and late stages of larvae, and refrigerate for later use.
[0022] 2. Production of host eggs
[0023] Start hanging cocoons around February 25th, and heat them under the conditions of 18-23°C and RH60-65%. After the moths emerge around March 17th, collect the female moths, take the eggs by caesarean section, wash and sq...
Embodiment 2
[0031] Take tussah silkworm cocoons as raw materials to produce the pine caterpillar Trichogramma, and use it in the field in mid-July to control the first-generation corn borer as an example:
[0032] 2. Production of bee species:
[0033] In the second half of December, warm the cocoons and moths. After the moths emerged around January 20, the eggs were collected by laparotomy, and then the bees were picked up at a ratio of 1:35-40. After picking up the bees in a dark room at 23-25°C and RH70-75% for 24 hours , remove bee species, transfer to 20-25°C variable temperature, RH70-75%, L16:D8 conditions to develop to the middle and late stages of larvae, and refrigerate for later use.
[0034] 2. Production of host eggs
[0035] Start to hang cocoons around March 5th, and heat them under the conditions of 18-23°C and RH60-65%. After the moths emerge around March 25th, collect the female moths, take the eggs by caesarean section, wash and squeeze the green eggs, Disinfect with ...
Embodiment 3
[0043] Taking tussah cocoons as raw materials to produce the pine caterpillar Trichogramma, the field application in August to control the second-generation corn borer is an example:
[0044] 1. Production of bee species:
[0045] In mid-January, the cocoons were warmed up and the moths were raised. After the moths emerged around February 8, the caesarean eggs were collected, and then the bees were picked up at a ratio of 1:35-40. After picking up the bees in a dark room under the conditions of 23-25°C and RH70-75% for 24 hours , remove bee species, transfer to 20-25°C variable temperature, RH70-75%, L16:D8 conditions to develop to the middle and late stages of larvae, and refrigerate for later use.
[0046] 2. Production of host eggs
[0047] Start hanging cocoons around March 26, and heat them under the conditions of 18-23°C and RH60-65%. After the moths emerge around April 15, collect the female moths, take the eggs by caesarean section, and wash and squeeze the green eggs...
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