Preparation method for polyphenol monomers from gnaphlium affine

A polyphenolic compound, the technology of Shamrock, which is applied in the field of preparation of polyphenolic compound monomers in Shamrock, can solve the problem of less research on compounds, and achieve the effect of simple method, simple method operation, and high purity

Inactive Publication Date: 2015-01-07
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the chemical constituents of S. sageris reported in the literature are mainly flavonoids and flavonoid glycosides, while there are few studies on other compounds. In-depth research on the types of compounds in S. Active relationship is extremely necessary

Method used

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  • Preparation method for polyphenol monomers from gnaphlium affine
  • Preparation method for polyphenol monomers from gnaphlium affine
  • Preparation method for polyphenol monomers from gnaphlium affine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for preparing polyphenolic compound monomers in Shamrock, the steps are:

[0019] (1) Fresh sage grass is dried in an oven at 60°C and crushed to 10 meshes, then the pulverized raw material is added with 20 times the amount of sage grass powder and ethanol with a mass concentration of 50%, and refluxed at 70°C Extract for 0.5h, filter, repeat the extraction of the filter residue once, combine the extracts and concentrate under reduced pressure until there is no ethanol smell, and at the same time adjust the pH = 3 with hydrochloric acid, filter to remove the precipitate, pass the filtrate through the layer containing D101 resin at a flow rate of 4BV / h Column analysis, the volume of the upper column is 0.5h after adsorption, washed with water until colorless, then eluted with 4BV mass concentration of 60% ethanol at a flow rate of 2BV / h, collected ethanol eluate, concentrated under reduced pressure and freeze-dried , to obtain the purified product of sagebrush t...

Embodiment 2

[0023] (1) Fresh sagebrush is dried in an oven at 60°C and crushed to 10 meshes, then the pulverized raw material is added with 25 times the amount of sagebrush powder and ethanol with a mass concentration of 60%, and refluxed at 75°C Extract for 1 hour, filter, and repeat the extraction of the filter residue once, combine the extracts and concentrate under reduced pressure until there is no ethanol smell, and at the same time adjust the pH to 3 with hydrochloric acid, filter to remove the precipitate, and pass the filtrate through the chromatography equipped with D101 resin at a flow rate of 4BV / h Column, the upper column volume is 0.5h after adsorption, washed with water until colorless, then eluted with 5BV mass concentration of 60% ethanol at a flow rate of 3BV / h, collected the ethanol eluate, concentrated under reduced pressure and freeze-dried. Obtain the product of sagebrush through the column purification;

[0024](2) Fully mix n-hexane, ethyl acetate, methanol, and gl...

Embodiment 3

[0027] (1) Fresh sage grass is dried in an oven at 60°C and crushed to 20 meshes, then the pulverized raw material is added with 30 times the amount of sage grass powder and ethanol with a mass concentration of 70%, and refluxed at 80°C Extract for 1 hour, filter, and repeat the extraction of the filter residue once, combine the extracts and concentrate under reduced pressure until there is no ethanol smell, and at the same time adjust the pH to 3 with hydrochloric acid, filter to remove the precipitate, and pass the filtrate through the chromatography equipped with D101 resin at a flow rate of 4BV / h Column, the upper column volume is 0.5h after adsorption, washed with water until colorless, and then eluted with 6BV mass concentration of 60% ethanol at a flow rate of 4BV / h, collected ethanol eluate, concentrated under reduced pressure and freeze-dried. Obtain the product of sagebrush through the column purification;

[0028] (2) Fully mix n-hexane, ethyl acetate, methanol, and...

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Abstract

The invention relates to the technical field of extraction of effective components from plants, and specifically relates to a preparation method for polyphenol monomers from gnaphlium affine. The preparation method comprises the steps of (1) preparing a column product of the gnaphlium affine; (2) loading a sample for elution; and (3) carrying out structure determination. Six compounds are prepared and separated from the gnaphlium affine by using HSCCC combined with preparative HPLC technology. The method is simple, efficient and non-toxic, and is suitable for large-scale preparation of the compounds. Five compounds are identified. Meanwhile, an inhibition effect of the compounds on alpha-glucosidase is studied; and a foundation is laid for pharmacological research and quality control of the gnaphlium affine. The method can extract the polyphenol monomers from the gnaphlium affine rapidly, and is simple to operate. The prepared polyphenol monomers have high purity.

Description

technical field [0001] The invention relates to the technical field of extracting active ingredients from plants, in particular to a method for preparing polyphenolic compound monomers from Sage sage. Background technique [0002] Gnaphalium affine (Gnaphalium affine D.Don) is the whole herb of Gnaphalium affine D. Don, a plant of the genus Gnaphalium affine in the family Asteraceae. Traditional medicine believes that it has the effects of relieving cough and reducing phlegm, dispelling wind and dampness, and detoxifying. In Mexico, sagebrush is often used as an anti-inflammatory drug. Modern pharmacological studies have further shown that S. sageria not only has significant anti-inflammatory, anti-oxidant, bacteriostatic, antitussive and expectorant effects, liver protection, etc., but also has insecticidal effects, so it has a wide range of development and utilization prospects. [0003] At present, the chemical constituents of S. sageris reported in the literature are m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/56C07H15/203C07H1/08
CPCC07C67/56C07H1/08C07H15/203C07C69/732
Inventor 陆英刘仲华李觅路谭斌林海燕李佳银
Owner HUNAN AGRICULTURAL UNIV
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