Preparation and Application of Polysaccharides from Amlica emblica
A technology of emblica and polysaccharides, applied in the field of preparation and application of emblica polysaccharides, can solve the problems of low decolorization rate and low polysaccharide content, and achieve the effect of good anti-tumor activity and inhibition of anti-tumor activity
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Embodiment 1
[0040] The seeds of emblica were removed and soaked in hot water at 60°C for 20 minutes. Then make a slurry, heat and reflux at a material-to-liquid ratio of 1:8 for 3 hours, filter the hot extract while it is hot, centrifuge (3000 r / min, 10 min), concentrate in vacuum to 1 / 4 of the original volume, and adjust to the solution with 95% edible alcohol The ethanol content was 80%, let it stand overnight, centrifuged (3000 r / min, 10 min), the precipitate was washed twice with an appropriate amount of 80% ethanol and absolute ethanol in turn, and vacuum freeze-dried to obtain the crude polysaccharide of Amla emblica for later use.
[0041] The crude polysaccharides of Amla emblica were prepared into a 0.5 mg / ml sample solution of emblica polysaccharides after decolorization.
Embodiment 2
[0043] The crude polysaccharides in the examples were formulated into 0.5 mg / ml emblica crude polysaccharide solution, and 50.00 ml sample solution of emblica polysaccharide was taken to investigate different decolorization methods. Method 1: Add 2% activated carbon to the sample solution, adjust the pH to 4.0 with citric acid, stir in a water bath at 60°C for 30 minutes, and filter to remove the residue; Method 2: Add 12.5ml of H2O2 solution to the sample solution (the ratio of H2O2 solution to polysaccharide solution is 1:4 , V / V), adjust the pH to 9.0 with 1% NaOH or concentrated ammonia water, heat and stir in a water bath at 60°C for 3 h, then heat to remove hydrogen peroxide; method three: add 1.5 g / 100ml of sample solution to the insoluble For polysaccharides, adjust the pH to 4.0 with citric acid, decolorize with intermittent stirring in a water bath at 60°C for 30 min, and remove the residue by filtration. The decolorization rate and polysaccharide content were measur...
Embodiment 3
[0048] Prepare the rough sugar in the example into 0.5 mg / ml emblica crude polysaccharide solution, adjust the pH to 4 with citric acid, add chitosan 1.0 g, 2.0 g, 3.0 g, 4.0 g to each 100 ml emblica polysaccharide solution g, 5.0 g, decolorized in a water bath at 60°C for 30 min with intermittent stirring, filtered to remove the residue, measured and calculated the decolorization rate and polysaccharide content, the results are shown in Table 4.
[0049] Table 4 The effect of chitosan dosage on polysaccharide decolorization rate and polysaccharide content
[0050]
[0051] It can be seen from Table 4 that when the amount of chitosan is 2.0 g to 3.0 g, the decolorization rate increases greatly while the polysaccharide content changes little; Considering that the amount of chitosan should be controlled between 2.0g and 3.0g (per 100 ml emblica polysaccharide, the concentration is 0.5 mg / ml).
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