Method for producing chiral pyridine ethanol through biological catalysis
An ethanol and pyridyl technology, applied in the field of pharmaceutical engineering, can solve problems such as high cost and difficult chemical synthesis
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0019] Prepare wet cyanobacteria by conventional methods ATCC 52264 Cells, used as catalysts for biocatalytic reactions.
[0020] The method for making the cotton gauze that has adsorbed the substrate is that the substrate 3-acetylpyridine is absorbed by cotton gauze with a size of 0.5cm×0.5cm to obtain the cotton gauze that has adsorbed the substrate, and the amount of substrate and cotton gauze is adjusted so that The mass ratio of substrate to cotton gauze was 0.35. Cotton gauze used is common cotton gauze, after buying from the market, be cut into 0.5cm * 0.5cm fritter, sterilize, aseptic preservation stand-by, other embodiment cotton gauze handles identically.
[0021] Add phosphate buffer solution to a 15L bottom ventilated stirring reaction tank, the filling coefficient is 0.6, the pH is 5, add the cotton gauze that has adsorbed the substrate 3-acetylpyridine, so that the substrate 3-acetylpyridine is added in an amount of 80 g / L, the content of corn steep liquor (i...
Embodiment 2
[0023] Prepare wet cyanobacteria by conventional methods ATCC 52264 Cells, used as catalysts for biocatalytic reactions.
[0024] The method for making the cotton gauze that has adsorbed the substrate is that the substrate 3-acetylpyridine is absorbed by cotton gauze with a size of 0.5cm×0.5cm to obtain the cotton gauze that has adsorbed the substrate, and the amount of substrate and cotton gauze is adjusted so that The mass ratio of substrate to cotton gauze was 0.33. Cotton gauze used is common cotton gauze, after buying from the market, be cut into 0.5cm * 0.5cm fritter, sterilize, aseptic preservation stand-by, other embodiment cotton gauze handles identically.
[0025] Add phosphate buffer solution to the 100L bottom ventilated stirring reaction tank, the filling coefficient is 0.6, the pH is 5, add the cotton gauze that has adsorbed the substrate 3-acetylpyridine, so that the substrate 3-acetylpyridine is added in an amount of 90 g / L, the content of corn steep liquor...
Embodiment 3
[0028] Prepare wet cyanobacteria by conventional methods ATCC 52264 Cells, used as catalysts for biocatalytic reactions.
[0029]The method for making the cotton gauze that has adsorbed the substrate is that the substrate 3-acetylpyridine is absorbed by cotton gauze with a size of 0.5cm×0.5cm to obtain the cotton gauze that has adsorbed the substrate, and the amount of substrate and cotton gauze is adjusted so that The mass ratio of substrate to cotton gauze was 0.35. Cotton gauze used is common cotton gauze, after buying from the market, be cut into 0.5cm * 0.5cm fritter, sterilize, aseptic preservation stand-by, other embodiment cotton gauze handles identically.
[0030] Add phosphate buffer solution to the 500L bottom ventilated stirring reaction tank, the filling coefficient is 0.6, the pH is 5, add the cotton gauze that has adsorbed the substrate 3-acetylpyridine, so that the substrate 3-acetylpyridine is added in an amount of 85g / L, the content of corn steep liquor (...
PUM
| Property | Measurement | Unit |
|---|---|---|
| size | aaaaa | aaaaa |
| size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More