Trichoderma capable of preventing and treating root rot of loquat

A technology of loquat root and Trichoderma, which is applied in the field of microorganisms, can solve problems such as being difficult to achieve, and achieve the effect of strong antagonistic effect, large ecological benefit, and no environmental pollution.

Inactive Publication Date: 2015-01-21
HONGHE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Searching for genes resistant to loquat root rot and successfully breeding resistant varieties against loquat root rot is difficult to achieve in the short term

Method used

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  • Trichoderma capable of preventing and treating root rot of loquat
  • Trichoderma capable of preventing and treating root rot of loquat
  • Trichoderma capable of preventing and treating root rot of loquat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Inoculate the LY1 Trichoderma strain in the center of a 90mm-diameter petri dish filled with PDA medium in advance, culture at 28°C for 5 days, and use a 5mm-diameter punch to take out the fungus cake for later use. The main phloem of the seedling is punched out, and the phloem is removed; the above-mentioned bacteria cake is placed in the loquat trunk hole from which the phloem has been removed, and sealed and kept moist with a plastic wrap. PDA nutrient cakes inoculated with the same diameter were used as a control. Routine fertilizer and water management was performed during the experiment. On the 30th day of inoculation, use a puncher with a diameter of 5 mm between the two inoculation points where the LY1 Trichoderma cake was inoculated, and take its phloem, and take 3 pieces for each plant for use. Disinfect with 74% alcohol for 1 min, 0.1% mercuric chloride for 1 min, rinse with sterile water for 3 times, blot the water with sterilized filter paper, inoculate on...

Embodiment 2

[0028] Trichoderma LY1 and loquat root rot strains (Pestalotiopsis sp.) were inoculated in the center of a 90 mm-diameter petri dish filled with PDA medium in advance, cultured at 28°C for 5 days, and then respectively Bacteria cakes with the same radius were taken and inoculated on a 90mm-diameter petri dish filled with PDA medium at the same time. The distance between the two inoculation points was 45mm. As a control, the cakes of loquat root rot fungus were inoculated separately, cultured at 28°C for 7 days, and measured. Root rot fungus colony diameter. The formula for calculating the inhibition rate is:

[0029] Inhibition rate / %=[(dCK-dB) / dCK]×100

[0030] In the formula, dCK represents the colony diameter of the control pathogenic bacteria, and dB represents the colony diameter of the treated pathogenic bacteria.

[0031]Measurement result dCK=90mm, dB=10.8mm, so inhibition rate=88%; Find that LY1 heavy parasitism on loquat root rot bacterial colony simultaneously ( ...

Embodiment 3

[0032] Example 3 The method of preventing and treating loquat root rot at the seedling stage

[0033] Inoculate the LY1 Trichoderma strain and the loquat root rot strain in the center of a 90mm-diameter petri dish filled with PDA medium in advance, culture at 28°C for 5 days, and use a 5mm-diameter punch to take out the fungus cake for later use. Punch out holes in the main phloem of loquat seedlings and remove the phloem; place the above-mentioned bacterium cake in the loquat trunk hole where the phloem was removed, and inoculate 5 bacterium cakes per plant, between two inoculation points, use the same method to inoculate 4 loquat root rot bacteria cakes, sealed with plastic wrap to keep moisture. Set only the plants inoculated with 5 loquat root rot bacteria cakes as the control, and the inoculation methods were the same. Routine fertilizer and water management was performed during the experiment. On the 12th day of inoculation, it was observed that the control leaves turn...

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PUM

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Abstract

The invention discloses trichoderma capable of preventing and treating root rot of a loquat and belongs to the technical field of microorganisms. Preservation number of the trichoderma capable of preventing and treating the root rot of the loquat is CGMCC No.9390. The trichoderma has a strong antagonistic effect on pathogenic bacteria of the root rot of the loquat and can be used for preventing and treating the root rot of the loquat, and indoor inhibition ratio reaches up to 88%. A method for preventing and treating the root rot of the loquat by adopting the trichoderma comprises the following steps: culturing the trichoderma on a solid culture medium, punching a bacterium cake for later use by adopting a puncher, punching a hole in the phloem of a trunk of a loquat seedling by adopting the puncher in the same specification at the same time, and removing the phloem; placing the bacterium cake into the hole in the trunk, the phloem of which is removed, of the loquat, and sealing with a preservative film for moisturizing; or culturing the trichoderma in a liquid culture medium, diluting a bacterium solution with sterilize water, and irrigating each loquat for 500ml diluent at roots. The trichoderma disclosed by the invention can be used for effectively preventing and treating the root rot of the loquat, a prevention and control method is simple, the environment is not polluted, and growth of the loquat can be promoted.

Description

technical field [0001] The invention belongs to the technical field of microbes and relates to a Trichoderma strain capable of preventing and treating loquat root rot. Background technique [0002] Loquat (Eriobotrya japonica) is distributed in the south of the Yangtze River in China and is one of the main fruit trees in southern China. Its flower, fruit, leaf, root and bark etc. can be used as medicine. The fruit pulp is soft and juicy, moderately sweet and sour, and rich in nutrition. China's loquat production accounts for more than 70% of the world's total output, and planting loquats is becoming a way to get rid of poverty and become rich in southern mountainous areas. [0003] Unfortunately, loquats in Yunnan, Fujian, Taiwan and other provinces and cities are harmed by root rot. Only in Mengzi City, Yunnan Province, the incidence of loquat root rot is as high as 40%, and the annual loss is more than 8 million yuan. Years of investigations by the School of Life Scienc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00A01P21/00A01G7/06C12R1/885
CPCA01G7/06A01N63/30C12N1/145C12R2001/885
Inventor 鲁海菊刘艳红陶红征沈云玫杨建洪亮王栋
Owner HONGHE COLLEGE
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