Chrysanthemum aphid resistance associated molecular marker, screening method and application
A technology of molecular markers and screening methods, applied in biochemical equipment and methods, DNA/RNA fragments, microbial determination/inspection, etc., can solve complex genetic mechanisms of aphid resistance and large differences in genetic control mechanisms of aphid resistance, etc. question
Active Publication Date: 2015-01-28
NANJING AGRICULTURAL UNIVERSITY
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[0004] Studies have found that the genetic mechanism of aphid resistance in plants is relatively complex, not only affected by additive, epistatic interaction effects and environmental factors, but also the genetic control mechanism of aphid resistance in different crops is quite different
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[0069] The present invention will be further described below in conjunction with the examples, and the experimental methods that do not specify specific conditions in the following examples generally follow the known methods in the art.
[0070] (1) Acquisition of test materials and their phenotypic data
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The invention belongs to the field of biotechnology and provides a chrysanthemum aphid resistance associated molecular marker, a molecular marker primer, a molecular marker screening method and the application thereof in breeding new varieties of chrysanthemum. The chrysanthemum aphid resistance associated molecular marker comprises molecular markers of main effect QTL sites NoaE2G1, NoaE2G7, NoaE1H3, NoaE2H7 and NoaE2H8. The screening method comprises: (1) obtaining a test material and phenotypic data of the test material; (2) building a chrysanthemum linkage map; (3) QTL positioning the chrysanthemum aphid resistance traits; (4) determining the chrysanthemum aphid resistance associated molecular marker. By adopting the invention, the current situation that research on the accurate positioning and cloning of the chrysanthemum aphid resistance gene is nearly blank at home and abroad is solved, the molecular marker firmly associated with the chrysanthemum aphid resistance gene is screened, and an auxiliary selection system of the chrysanthemum aphid resistance associated molecular marker is established, to improve the selection efficiency of chrysanthemum aphid resistance and lay a foundation for breeding new varieties of chrysanthemum.
Description
technical field [0001] The invention belongs to the field of biotechnology, and relates to molecular markers related to aphid resistance of chrysanthemums, a screening method and the application of the above two in the positioning and cloning of excellent genes of chrysanthemum resistance to aphids and the cultivation of new varieties of chrysanthemums. Background technique [0002] Chrysanthemum (Chrysanthemum morifolium Ramat.) is a long-term artificially cultivated traditional flower in my country, which has medicinal, edible, ornamental and health value. Aphids are one of the main pests that harm chrysanthemums, among which Aphid chrysanthemum is the most serious. Aphids not only affect the ornamental value of chrysanthemums, but also greatly reduce the yield and quality of chrysanthemums, causing great economic losses. At present, the control of aphids mostly adopts chemical methods, but the control of pesticides not only consumes manpower and material resources, cause...
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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/686C12Q1/6895C12Q2600/124C12Q2600/156
Inventor 陈发棣王楚楚苏江硕张飞管志勇陈素梅王海滨房伟民
Owner NANJING AGRICULTURAL UNIVERSITY