Primer pair for simultaneously detecting CBFB-MYH11 and AML1-ETO fusion genes with pyrosequencing method and kit

Abstract

The invention discloses a primer pair for simultaneously detecting CBFB-MYH11 and AML1-ETO fusion genes with a pyrosequencing method and a kit containing the primer pair. The kit can be used to detect multiple fusion genes of CBFB-MYH11 (type A), CBFB-MYH11(type D) and AML1-ETO in one step. By means of designing primers for mRNA of CBFB-MYH11 and AML1-ETO fusion genes and performing sequencing analysis on a product after reverse-transcription PCR, the kit can realize the rapid, accurate and high-throughput detection on the CBFB-MYH11 and AML1-ETO fusion genes.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a primer pair for simultaneous detection of CBFB-MYH11 and AML1-ETO fusion genes by a pyrosequencing method, a kit containing the primer pair, and an application of the kit. Background technique [0002] CBFB-MYH11 and AML1-ETO fusion genes include CBFB-MYH11 (type A), CBFB-MYH11 (type D), AML1-ETO and other types, which are common in acute myeloid leukemia (AML). [0003] Among the molecular biology methods widely used at home and abroad to detect CBFB-MYH11 and AML1-ETO fusion genes, fluorescence in situ hybridization (FISH) can only perform qualitative detection, and the operation is complicated; fluorescent quantitative PCR has limitations in detection throughput Therefore, they cannot really meet the needs of clinical diagnostic testing. The traditional solid-phase biochip (Biochip) or gene chip technology has outstanding weaknesses such as poor repeatability, i...

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Problems solved by technology

[0003] Among the molecular biology methods widely used at home and abroad to detect CBFB-MYH11 and AML1-ETO fusion genes, fluorescence in situ hybridization (FISH) can only perform qualitative detection, and the operation is complicated; fluorescent quantitative PCR has limitatio

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