Lamp primer, kit and method for detecting murine Staphylococcus aureus

Abstract

The invention discloses LAMP primers, a kit and a method for detecting staphylococcus aureus of rats. The LAMP primers are designed according to the aroE gene sequence of staphylococcus aureus and further a visual detection method for staphylococcus aureus of rats, which is applicable to clinical sample detection, high specificity and high sensitivity can be established; and the existence of staphylococcus aureus can be detected rapidly by observing whether green fluorescence appears or not and the lowest detection limit is 8.1 copy number / reaction. By virtue of the recognition of the four primers designed and screened by the invention to specific sequence regions of target sequences, the high specificity of LAMP is guaranteed, the amplification efficiency is improved greatly, the time consumption is low; and therefore, the LAMP primers are suitable for field application at a primary level.

Description

technical field [0001] The invention belongs to the technical field of medical microorganism detection, and relates to a LAMP primer and a detection method for detecting murine Staphylococcus aureus. Background technique [0002] Staphylococcus aureus is a Gram-positive, non-spore-forming, flagellar, mostly non-encapsulated, lactic acid fermenting, facultatively anaerobic spherical bacterium. Staphylococcus aureus is commonly found on skin surfaces and upper respiratory mucosa. As an opportunistic pathogen, Staphylococcus aureus is a normal flora of the epidermis, often causing opportunistic infections, and the drug-resistant strains of the bacteria have brought greater difficulty to antibiotic treatment, and are common health monitoring indicators for experimental rodents one. [0003] Currently commonly used detection methods for Staphylococcus aureus include the direct plating method using monitoring swabs, chromogenic medium method and conventional PCR method / real-time...

AI Technical Summary

Problems solved by technology

However, compared with other methods, the bacterial culture method has disadvantages such as difficulty in standardization, long time-consuming, medium, operation steps or culture conditions are quite different in different laboratories, so the results may be inconsistent or false negative / positive

The conventional PCR method and the fluorescent quantitative PCR method need to go through denaturation, annealing, extension, and the whole process of DNA extraction takes about 2 to 4 hours to produce results, and require sophisticated and expensive instruments and reagents to quickly and easily carry out molecular unfavorable diagnosis

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