Lamp primer, kit and method for detecting murine Staphylococcus aureus
A staphylococcus, golden yellow technology, applied in the field of medical microorganism detection, can solve the problems of unfavorable molecular diagnosis, inconsistent results, false negative/positive, etc., and achieve the effects of rapid detection, convenient results, and easy operation.
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[0030] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments, which are explanations rather than limitations of the present invention.
[0031] The invention discloses a LAMP method for detecting murine Staphylococcus aureus used in experiments. The detection method is based on loop-mediated isothermal amplification.
[0032] According to the specific base sequence of the conserved gene aroE of Staphylococcus aureus, the present invention designs specific primers including outer primers and inner primers. Simply add the sample to the established LAMP reaction system to quickly and accurately detect the presence of Staphylococcus aureus by observing the presence or absence of green fluorescence. Provides a convenience method suitable for the base layer.
[0033] 1. Primer design and synthesis:
[0034] Considering the specificity and accuracy of the detection, according to literature reports, the specific base se...
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