Method for stereo-selectively preparing ursodesoxycholic acid

A technology of ursodeoxycholic acid and stereoselectivity, applied in electrolysis process, electrolysis components, electrolysis organic production, etc., can solve the problems of immature electrochemical synthesis methods, achieve high-efficiency preparation, simple operating conditions, and specificity increased effect

Active Publication Date: 2015-02-04
EAST CHINA UNIV OF SCI & TECH
View PDF9 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current electrochemical synthesis method is still immature

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for stereo-selectively preparing ursodesoxycholic acid
  • Method for stereo-selectively preparing ursodesoxycholic acid
  • Method for stereo-selectively preparing ursodesoxycholic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Dissolve 0.3g KBr in 12ml of water, 2.0g of 7-ketolithocholic acid in 110ml of methanol, and add 10ml of dimethylformamide, then mix the two solutions into the cathode cell as the catholyte; 10% dilute sulfuric acid is used as the anolyte; the lead electrode is used as the cathode, and the ruthenium-titanium electrode network is used as the anode; the HF101 strong-acid cation exchange membrane is used as the diaphragm, and the water bath is 50°C, and the constant current is electrolyzed, and the current density is 85A m -2 , Stop electrolysis when 7K-LCA disappears after thin layer chromatography. 1.932 g of UDCA crude product was obtained, the conversion rate by HPLC was 89.1%, and the purity was 52.3%.

Embodiment 2

[0029] Dissolve 0.3g KBr in 12ml water, dissolve 2.0g 7-ketolithocholic acid in 110ml methanol and add 5ml dimethyl sulfoxide, then mix the two solutions and put them into the cathode cell as catholyte; 5% dilute sulfuric acid is used as the anolyte; the lead electrode is used as the cathode, the ruthenium-titanium electrode network is used as the anode; the HF101 strong acid type cation exchange membrane is used as the diaphragm, the water bath is 65°C, and the constant current is electrolyzed, and the current density is 82A m -2 , Stop electrolysis when 7K-LCA disappears after thin layer chromatography. 1.894 g of crude UDCA was obtained, the conversion rate was 88.2% and the purity was 78.7% as measured by HPLC.

Embodiment 3

[0031] Dissolve 0.3g KBr in 12ml water, dissolve 2.5g 7-ketolithocholic acid in 110ml ethanol and add in 3ml dimethylacetamide, then mix the two solutions and put them into the cathode cell as catholyte; The 5% dilute sulfuric acid is used as the anolyte; the lead electrode is used as the cathode, and the PbO2 / Ti electrode mesh is used as the anode; the HF101 strong acid type cation exchange membrane is used as the diaphragm, and the water bath is 60°C, and the constant current is electrolyzed, and the current density is 85A m -2, Stop electrolysis when 7K-LCA disappears after thin layer chromatography. 1.914 g of crude UDCA was obtained, the conversion rate of the raw material detected by HPLC was 74.3%, and the purity was 55.1%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for stereo-selectively preparing ursodesoxycholic acid. The method is characterized by comprising following steps: (1) dissolving a compound represented as the formula (I) in a mixed solution consisting of an organic solvent and a stereo-selective reagent to prepare an electrolyte, wherein the compound represented as the formula (I) has a structure as follows; (2) in the presence of the stereo-selective reagent, performing current-constant reduction to the electrolyte with a current density being 75-100 A*m<-2> to obtain a solution contianign the ursodesoxycholic acid represented as the formula (II), wherein the ursodesoxycholic acid represented as the formula (II) has a structure as follows. By means of the method for stereo-selectively preparing the ursodesoxycholic acid, the ursodesoxycholic acid in high purity can be obtained. The method is high in yield, is short in preparation process, is high in efficiency, is simple in operation conditions and is free of environmental pollution.

Description

technical field [0001] The invention relates to the field of preparation of medicines by electrochemical reduction, in particular to the artificial synthesis of natural medicines, in particular to a method for electrochemical stereoselective preparation of ursodeoxycholic acid. Background technique [0002] Ursodeoxycholic acid is mainly used to treat various diseases such as gallstones, cholecystitis, hepatitis and hyperlipidemia, and is the only drug approved by the FDA for the treatment of primary biliary cirrhosis (PBC). Ursodeoxycholic acid is the main active ingredient of the precious traditional Chinese medicine bear bile. However, bear bile is a very scarce resource, which is difficult to meet the needs of clinical application. Since the 1950s, there have been chemical synthesis methods using animal bile acids as raw materials. Japanese patent (JP 0161496) reported that taking bovine and sheep cholic acid as raw materials, selectively protecting the 3-position hydr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C25B3/04C25B3/25
Inventor 曹学君黄晓梅董东东
Owner EAST CHINA UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap