Hydrogen peroxide measurement method based on N-acetyl-L-cysteine-gold nanoclusters
A technology of gold nanoclusters and cysteine, which is applied in the field of hydrogen peroxide determination, can solve the problems of high biological toxicity, poor photostability, and high cost, and achieve the effect of fast preparation process, high sensitivity, and short analysis time
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example 1
[0034] Add 0.6 mL of 0.5 mol / L sodium hydroxide solution and 0.4 mL of 0.02 g / L chloroauric acid solution to 4 mL of 0.08 mol / L N-acetyl-L-cysteine solution , mixed well, and placed in a 37°C constant temperature water bath to react for 2.5 h. After the reaction, the reaction solution was purified with a dialysis bag with a molecular weight cut off of 3500. The obtained gold nanocluster solution is colorless under visible light, and produces strong red fluorescence under ultraviolet light irradiation. Stored in the dark at 4°C, it can remain relatively stable for at least one month.
example 2
[0036] Get 0.2 ml of the gold nanocluster solution prepared in Example 1 and add to 0.2 ml concentration of 25 μmol / L H 2 o 2 solution (pH=3), after mixing evenly, add 0.05 ml of Fe with a concentration of 0.9 mmol / L 2+ , shake well and place in a 25°C water bath for 10 minutes. Observed under ultraviolet light, the gold nanocluster solution itself exhibits red fluorescence ( figure 1 In A), the red fluorescence of the gold nanocluster solution is quenched after the Fenton reaction ( figure 1 in B). figure 2 is the gold nanocluster solution ( figure 2 A) in A) and the gold nanocluster solution after the Fenton reaction ( figure 2 In B) Fluorescence emission spectrum graph.
example 3
[0038] Get 0.2 ml of the gold nanocluster solution prepared in Example 1 and add to 0.2 ml concentration of 100 μmol / L H 2 o 2 solution (pH=3), after mixing evenly, add 0.05 ml of Fe with a concentration of 0.9 mmol / L 2+ , shake well and place in a 25°C water bath for 10 minutes. The solution after the reaction is freeze-dried, and the dried powder is taken for X-ray photoelectron spectroscopy analysis. Such as image 3 As shown, the 4f of gold after the Fenton reaction 7 / 2 The binding energy shifted from the original 84.57 eV to 84.75 eV, indicating that the hydroxyl radicals generated by the Fenton reaction oxidized the gold atoms, which led to the quenching of the fluorescence of the gold nanoclusters.
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