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Detection method of vitamin B12 content in vitamin complex additive

A multivitamin and detection method technology, applied in the field of detection of vitamin B12 content in multivitamin additives, can solve problems such as difficulty in detecting vitamin B12 content, and achieve the effects of high repeatability, simple operation and high accuracy

Inactive Publication Date: 2015-02-04
XIAN MIYI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the existing detection methods are generally difficult to detect the content of vitamin B12 in infant formula milk powder

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Prepare a vitamin B12 stock solution with a concentration of 1 mg / ml; then prepare solutions with a series of standard concentrations of 0.5, 1.0, 2.0, 3.0, 4.0, and 5.0 μg / ml, and the injection volume is 20 μL. Under certain chromatographic conditions (the chromatographic column is an ODS chromatographic column, the number of theoretical plates of the chromatographic column is 2216; the column temperature is room temperature; the flow rate of the mobile phase is 1ml / min; the measurement wavelength is 361nm; the mobile phase is sodium dihydrogen phosphate (10g / L pH=3.5):methanol=70:30 mixture) measured on the computer and calculated regression equation and correlation coefficient. The resulting standard curve is:

[0018] Y=-0.1340+0.0009592X, R=0.9976. The results showed that there was a positive correlation between B12 concentration and peak height within the measurement range, and the correlation was good, which could be used for quantitative determination by ex...

Embodiment 2

[0022] Embodiment two: repeatability test, recovery rate test and detection limit test

[0023] After determination, the method recovery rate and precision test results are better, the recovery rate is between 90-102%, an average of 96.4%, the coefficient of variation of the sample repeated measurement 12 times is 0.54%, the detection limit of this method is 4ng, and the calculation can be The detection limit of vitamin B12 in vitamin additives for infant formula milk powder is 1 μg / g, which can satisfy the determination of vitamin B12 in any vitamin additives for milk powder.

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Abstract

The invention relates to a detection method of the vitamin B12 content in a vitamin complex additive, which belongs to the fields of food additives and detection methods of the food additives. The detection method comprises the following steps: (1) preparing a vitamin B12 stock solution with the concentration of 1 mg / ml, then preparing serial solutions with the normal concentrations of 0.5, 1.0, 2.0, 3.0, 4.0 and 5.0mu g / ml, putting on an instrument to measure under certain chromatographic conditions, and calculating a regression equation and correlation coefficients; and (2) weighing a test sample, putting on the instrument to measure under the same chromatographic conditions, and calculating the concentration of the test sample according to the peak height of a standard sample, the concentration of the standard sample and the peak height of the test sample. According to the detection method, the measuring precision and accuracy can meet the requirement of dairy product analysis, and the detection method has the characteristics of high accuracy, high repeatability, simplicity and convenience in operation, low detection limit and the like and can be used as a conventional detection method to be applied.

Description

technical field [0001] The invention relates to a detection method for vitamin B12 content in a compound vitamin additive, and belongs to the field of food additives and detection methods thereof. Background technique [0002] Vitamin B12 is one of the largest and most complex molecules among all vitamins, but the human body's requirement for it may be the least among all vitamins, only about 3 micrograms per day. Excess vitamin B12 can also have side effects, and large amounts of vitamin B12 can also lead to folate deficiency. Vitamin B12 can improve the utilization rate of folic acid. If vitamin B12 is lacking, the utilization of folic acid will be affected. Vitamin B12 deficiency can also lead to peripheral neuritis. The early manifestations of vitamin B12 deficiency in children are abnormal mental mood, sluggish expression, less crying, slow reaction, love to sleep, unconscious movement of hands and feet or trembling of head and limbs, and finally lead to anemia. Expe...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 李勇刘文斌
Owner XIAN MIYI BIOTECH
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